AB229657

Anti-Lysozyme antibody

Name: Anti-Lysozyme antibody (ab229657) | Abcam
Brand: Abcam Limited
SKU: AB229657
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Lysozyme antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LYZ.

View Alternative Names

LZM, LYZ, Lysozyme C

2 Images

IHC - Wholemount - Anti-Lysozyme antibody (AB229657)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Lysozyme antibody (AB229657)

3 days-post-fertilization zebrafish embryo stained for Lysozyme (green) using ab229657 at 1/100 dilution in whole mount immunohistochemical analysis.

Supplier Data

Western blot - Anti-Lysozyme antibody (AB229657)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-Lysozyme antibody (ab229657) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Predicted band size: 16 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt, WB

applications

Immunogen

Recombinant Fragment Protein within Human LYZ. The exact immunogen used to generate this antibody is proprietary information.

P61626

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.

Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

See full target information LYZ

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:1743-1760.e11 PubMed37116497

2023

Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo José Longarini,Helen Dauben,Carolina Locatelli,Anne R Wondisford,Rebecca Smith,Charlotte Muench,Andreas Kolvenbach,Michelle Lee Lynskey,Alexis Pope,Juan José Bonfiglio,Eva Pinto Jurado,Roberta Fajka-Boja,Thomas Colby,Marion Schuller,Ivan Ahel,Gyula Timinszky,Roderick J O'Sullivan,Sébastien Huet,Ivan Matic

Environmental science & technology 56:4251-4261 PubMed35286074

2022

Antibiotic Chlortetracycline Causes Transgenerational Immunosuppression via NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Qiu,Bei Chen,Liang Tang,Chunmiao Zheng,Bentuo Xu,Zhiyu Liu,Jason T Magnuson,Shuwen Zhang,Daniel Schlenk,Elvis Genbo Xu,Baoshan Xing

Journal of fish diseases 45:19-33 PubMed34549432

2021

Implication of mucus-secreting cells, acidophilic granulocytes and monocytes/macrophages in the resolution of skin inflammation caused by subcutaneous injection of λ/κ-carrageenin to gilthead seabream (Sparus aurata) specimens.

Applications

Unspecified application

Species

Unspecified reactive species

José Carlos Campos-Sánchez,Enrica Vitarelli,Francisco A Guardiola,Diana Ceballos-Francisco,José María García Beltrán,Antonio Ieni,María Ángeles Esteban

View all publications

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Product promise

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