AB2408

Anti-Lysozyme antibody

Name: Anti-Lysozyme antibody (ab2408) | Abcam
Brand: Abcam Limited
SKU: AB2408
Price: 550 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(11 Publications)

Rabbit Polyclonal Lysozyme antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LYZ.

View Alternative Names

LZM, LYZ, Lysozyme C

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody (AB2408)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody (AB2408)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Lysozyme with ab2408.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody (AB2408)

ab2408 staining lysozyme in human colon. Staining is localised to cytoplasm.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human LYZ.

P61626

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.

Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

See full target information LYZ

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 11:1457719 PubMed39386251

2024

Wuzhishan miniature pig-derived intestinal 2D monolayer organoids to investigate the enteric coronavirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Liu,Ning Yang,Chen Tan,Yunhang Zhang,Shuai Gao,Yifei Cai,Yue Zhang,Yuguang Fu,Guangliang Liu,Yang Li

Cell reports 43:113975 PubMed38507411

2024

SIRT4 loss reprograms intestinal nucleotide metabolism to support proliferation following perturbation of homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Tucker,Song-Hua Hu,Sejal Vyas,Albert Park,Shakchhi Joshi,Aslihan Inal,Tiffany Lam,Emily Tan,Kevin M Haigis,Marcia C Haigis

iScience 26:106910 PubMed37378323

2023

Prolonged dysbiosis and altered immunity under nutritional intervention in a physiological mouse model of severe acute malnutrition.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Hidalgo-Villeda,Matthieu Million,Catherine Defoort,Thomas Vannier,Ljubica Svilar,Margaux Lagier,Camille Wagner,Cynthia Arroyo-Portilla,Lionel Chasson,Cécilia Luciani,Vincent Bossi,Jean-Pierre Gorvel,Hugues Lelouard,Julie Tomas

JCI insight 8: PubMed37014710

2023

RBM47 regulates intestinal injury and tumorigenesis by modifying proliferation, oxidative response, and inflammatory pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Saeed Soleymanjahi,Valerie Blanc,Elizabeth A Molitor,David M Alvarado,Yan Xie,Vered Gazit,Jeffrey W Brown,Kathleen Byrnes,Ta-Chiang Liu,Jason C Mills,Matthew A Ciorba,Deborah C Rubin,Nicholas O Davidson

Nature protocols 17:378-401 PubMed35022622

2022

IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues.

Applications

mIHC

Species

Human

Andrea J Radtke,Colin J Chu,Ziv Yaniv,Li Yao,James Marr,Rebecca T Beuschel,Hiroshi Ichise,Anita Gola,Juraj Kabat,Bradley Lowekamp,Emily Speranza,Joshua Croteau,Nishant Thakur,Danny Jonigk,Jeremy L Davis,Jonathan M Hernandez,Ronald N Germain

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2004850 PubMed34240584

2021

An MST4-pβ-Catenin Signaling Axis Controls Intestinal Stem Cell and Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Moubin Lin,Chao Dong,Yang Tang,Liwei An,Junyi Ju,Fuping Wen,Fan Chen,Meng Wang,Wenjia Wang,Min Chen,Yun Zhao,Jixi Li,Steven X Hou,Xinhua Lin,Lulu Hu,Wenbo Bu,Dianqing Wu,Lin Li,Shi Jiao,Zhaocai Zhou

Proceedings of the National Academy of Sciences of the United States of America 117:33455-33465 PubMed33376221

2020

IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues.

Applications

mIHC

Species

Human

Andrea J Radtke,Evelyn Kandov,Bradley Lowekamp,Emily Speranza,Colin J Chu,Anita Gola,Nishant Thakur,Rochelle Shih,Li Yao,Ziv Rafael Yaniv,Rebecca T Beuschel,Juraj Kabat,Joshua Croteau,Jeremy Davis,Jonathan M Hernandez,Ronald N Germain

Nature biomedical engineering 4:544-559 PubMed32341538

2020

Robotically handled whole-tissue culture system for the screening of oral drug formulations.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas von Erlach,Sarah Saxton,Yunhua Shi,Daniel Minahan,Daniel Reker,Farhad Javid,Young-Ah Lucy Lee,Carl Schoellhammer,Tina Esfandiary,Cody Cleveland,Lucas Booth,Jiaqi Lin,Hannah Levy,Sophie Blackburn,Alison Hayward,Robert Langer,Giovanni Traverso

Journal of translational medicine 17:68 PubMed30823930

2019

Processed human amniotic fluid retains its antibacterial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Mao,Jan Pierce,Anya Singh-Varma,Michael Boyer,Joachim Kohn,Jo-Anna Reems

Biochemistry 55:1652-62 PubMed26673564

2016

Lysine-Specific Demethylase 1A (KDM1A/LSD1): Product Recognition and Kinetic Analysis of Full-Length Histones.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan M Burg,Julie J Gonzalez,Kenneth R Maksimchuk,Dewey G McCafferty

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB108508

Anti-Lysozyme antibody [EPR2994(2)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (AB108508)

Primary Antibodies

AB197704

Biotin Anti-Lysozyme antibody [EPR2994(2)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Lysozyme antibody [EPR2994(2)] (AB197704)

Primary Antibodies

AB197705

HRP Anti-Lysozyme antibody [EPR2994(2)]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lysozyme antibody [EPR2994(2)] (AB197705)

Primary Antibodies

AB185129

Anti-Lysozyme antibody [EPR2994(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] - BSA and Azide free (AB185129)

Primary Antibodies

AB227708

Anti-Lysozyme antibody [SP350] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Primary Antibodies

AB242420

Anti-Lysozyme antibody [SP329] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] - BSA and Azide free (AB242420)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB45179

Anti-Chromogranin A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (AB45179)

Primary Antibodies

AB272692

Anti-MUC2 antibody [EPR23479-47]

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com