Name: Anti-Lysozyme antibody [EPR2994(2)]
SKU: ab108508
Rating: 5 (4 reviews)

Anti-Lysozyme antibody [EPR2994(2)] (ab108508) is a rabbit monoclonal antibody detecting Lysozyme in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Clone EPR2994(2) is the most cited clone to Lysozyme

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 100 publications

Related conjugates and formulations

ab197704
Conjugated
Biotin Anti-Lysozyme antibody [EPR2994(2)]
ab197705
Conjugated
HRP Anti-Lysozyme antibody [EPR2994(2)]
ab185129
Carrier free
Anti-Lysozyme antibody [EPR2994(2)] - BSA and Azide free
ab313033
Conjugated
Alexa Fluor® 568 Anti-Lysozyme antibody [EPR2994(2)]
ab313234
Conjugated
Alexa Fluor® 555 Anti-Lysozyme antibody [EPR2994(2)]
ab311753
Conjugated
Alexa Fluor® 594 Anti-Lysozyme antibody [EPR2994(2)]
ab310997
Conjugated
Alexa Fluor® 488 Anti-Lysozyme antibody [EPR2994(2)]
ab311121
Conjugated
Alexa Fluor® 647 Anti-Lysozyme antibody [EPR2994(2)]
ab320903
Conjugated
Alexa Fluor® 750 Anti-Lysozyme antibody [EPR2994(2)]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (AB108508), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF
Human	Tested	Not recommended	Tested	Tested
Mouse	Tested	Not recommended	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000 - 1/5000	Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes For unpurified use at 1/500 - 1/1000.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

See full target information LYZ

Function

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

Alternative names

LZM, LYZ, Lysozyme C

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR2994(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-Lysozyme antibody [EPR2994(2)] (ab108508) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human, mouse samples.
Anti-Lysozyme antibody [EPR2994(2)] (ab108508) specifically detects Lysozyme (UniProt ID: P61626; Molecular weight: 15kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-Lysozyme antibody [EPR2994(2)] (ab108508) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Lysozyme antibody [EPR2994(2)] (ab108508) has been confirmed by testing in knockout samples.
Anti-Lysozyme antibody [EPR2994(2)] (ab108508) has been cited over 102 times in peer reviewed journals and is trusted by the scientific community.
Anti-Lysozyme antibody [EPR2994(2)] (ab108508) has 4 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone EPR2994(2) - ab185129.
Antibody clone EPR2994(2) is also available pre-conjugated to a variety of labels for your convenience - Biotin, HRP, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (ab197704, ab197705, ab310997, ab311121, ab311753, ab313033, ab313234, ab320903).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.

Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Associated diseases and disorders

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Immunohistochemical analysis of paraffin-embedded human lung tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody.Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Lanes 1 - 4: Merged signal (red and green). Green - ab108508 observed at 16 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab108508 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab108508 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution
Lane 1: Wild-type THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg
Lane 2: LYZ knockout THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human LYZ (Lysozyme) knockout THP-1 cell line (Human LYZ (Lysozyme) knockout THP-1 cell line ab269595)
Lane 3: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 16 kDa, 68 kDa, 71 kDa
Observed band size: 16 kDa, 58 kDa
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution
All lanes: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 10 kDa, 102 kDa, 11 kDa, 14 kDa, 16 kDa, 20 kDa, 202 kDa, 22 kDa, 24 kDa, 25 kDa, 29 kDa, 35 kDa, 36 kDa, 39 kDa, 41 kDa, 44 kDa, 55 kDa, 56 kDa, 59 kDa, 68 kDa, 72 kDa, 75 kDa, 81 kDa, 85 kDa, 9 kDa, 95 kDa
Observed band size: 10 kDa, 130 kDa, 16 kDa, 17 kDa, 210 kDa, 22 kDa, 25 kDa, 28 kDa, 29 kDa, 35 kDa, 47 kDa, 50 kDa, 52 kDa, 55 kDa, 72 kDa, 90-110 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia) cells labeling lysozyme with purified ab108508 at 1/250. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 g/mL) - Microtubule Marker (Alexa Fluor^®594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
This image is courtesy of a customer review submitted by Mr. Thorsten Cramer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Paraffin-embedded mouse small intestine tissue stained for Lysozyme using ab108508 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
All lanes: Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution
Lane 1: HL60 (human promyelocytic leukemia cell line) cell lysate at 10 µg
Lane 2: Human spleen lysate at 10 µg
Predicted band size: 16 kDa
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/50000 dilution
All lanes: HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 16 kDa, 48 kDa
Observed band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
All lanes: Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1 µg/mL
All lanes: Western blot - Native human Lysozyme protein (Native human Lysozyme protein ab91125) at 0.1 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Exposure time: 4min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Unpurified ab108508 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Unpurified ab108508 showing negative staining in Normal human breast tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
Unpurified ab108508 showing negative staining in Normal human heart tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.