Rabbit Recombinant Monoclonal Lysozyme antibody. Carrier free. Suitable for mIHC, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--western-blot-img141475.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136880.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137277.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430)")
![Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--flow-cytometry-intracellular-img52373.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87098.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (AB242430)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 products for Alternative Version
Target data
Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Alternative names
Lysozyme C, LYZ, LZM
Recommended products
Rabbit Recombinant Monoclonal Lysozyme antibody. Carrier free. Suitable for mIHC, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Lysozyme C, LYZ, LZM
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
SP350
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab242430 is the carrier-free version of Anti-Lysozyme antibody [SP350] - C-terminal ab227708.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.
- Biological function summary
Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.
- Pathways
Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.
- Associated diseases and disorders
Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--western-blot-img141475.jpg "Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Western blot - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Lysozyme antibody [SP350] - C-terminal ab227708 observed at 16 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
Anti-Lysozyme antibody [SP350] - C-terminal ab227708 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-Lysozyme antibody [SP350] - C-terminal ab227708 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (Anti-Lysozyme antibody [SP350] - C-terminal ab227708) at 1/400 dilution
Lane 1: Wild-type THP1 cell lysate at 20 µg
Lane 2: LYZ knockout THP1 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136880.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137277.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--flow-cytometry-intracellular-img52373.jpg "Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Intracellular flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA,and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87098.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137456.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137409.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136102.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using Anti-Lysozyme antibody [SP350] - C-terminal ab227708 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Multiplex immunohistochemistry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--multiplex-immunohistochemistry-img402763.jpg "Multiplex immunohistochemistry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
Multiplex immunohistochemistry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)
Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401, gray; Opal™690), anti-Lysozyme (ab242430, green; Opal™520) and anti-Chromogranin A (Anti-Chromogranin A antibody [EPR22537-249] ab254557, red; Opal™570) on human duodenum. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Lysozyme stained on Paneth cells. Panel D: anti-Chromogranin A stained on neuroendocrine cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 (1/8000 dilution), ab242430 (1:250 dilution), and Anti-Chromogranin A antibody [EPR22537-249] ab254557 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using the same antibody clone in a different buffer formulation (Anti-Lysozyme antibody [SP350] - C-terminal ab227708).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137456.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137409.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136102.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
![Multiplex immunohistochemistry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430), expandable thumbnail](https://content.abcam.com/products/images/lysozyme-antibody-sp350-bsa-and-azide-free-ab242430--multiplex-immunohistochemistry-img402763.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Lysozyme antibody [SP350] - BSA and Azide free (ab242430)")
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