Anti-Lysozyme antibody [SP350] - C-terminal

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-liver FABP (ab240401, gray; Opal™690), anti-Lysozyme (ab242430, green; Opal™520) and anti-Chromogranin A (ab254557, red; Opal™570) on human duodenum. Panel B : anti-liver FABP stained on enterocytes. Panel C : anti-Lysozyme stained on Paneth cells. Panel D : anti-Chromogranin A stained on neuroendocrine cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab240401 (1/8000 dilution), ab242430 (1 : 250 dilution), and ab254557 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Intracellular flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab227708 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

Lanes 1 - 4 : Merged signal (red and green). Green - ab227708 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab227708 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab227708 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution

Lane 1:

Wild-type THP1 cell lysate at 20 µg

Lane 2:

LYZ knockout THP1 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 16 kDa

Observed band size: 16 kDa

false

• WB

Supplier Data

Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (AB227708)

All lanes:

Western blot - Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Predicted band size: 16 kDa

false