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AB134912

Anti-LysRS antibody [EPR7920]

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(1 Publication)

Rabbit Recombinant Monoclonal LysRS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KARS, KIAA0070, KARS1, Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7920] (AB134912)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7920] (AB134912)

Immunohistochemical analysis of LysRs in paraffin embedded Human breast carcinoma tissue using ab134912 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7920] (AB134912)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7920] (AB134912)

Immunofluorescent analysis of LysRs in HeLa cells using ab134912 at a 1/250 dilution.

Western blot - Anti-LysRS antibody [EPR7920] (AB134912)
  • WB

Unknown

Western blot - Anti-LysRS antibody [EPR7920] (AB134912)

All lanes:

Western blot - Anti-LysRS antibody [EPR7920] (ab134912) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7920

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Complementary Products

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Primary Antibodies

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  • 0
  • 0
Primary Antibodies

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  • 0
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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody [EPR8525] (AB131054)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysine-tRNA ligase also known as LysRS plays a mechanical role in the translation process by facilitating the attachment of lysine to its corresponding tRNA a critical step for protein synthesis. LysRS is a class II aminoacyl-tRNA synthetase that contributes to the accuracy of genetic code translation. It is expressed in almost all cells as it is essential for protein production. The molecular weight of LysRS is approximately 68 kDa which reflects the complexity of its function and structure.
Biological function summary

Lysyl-tRNA synthetase participates in more than just translation. It is also involved in signal transduction pathways due to its ability to interact with other proteins. LysRS forms a multi-synthetase complex which includes several other aminoacyl-tRNA synthetases and auxiliary proteins. Through this complex it influences functions like gene expression and immune responses. The activity of LysRS outside of its synthetase function shows its versatility within cellular processes.

Pathways

Lysyl-tRNA synthetase is involved in critical pathways like mTOR signaling and aminoacyl-tRNA biosynthesis. These pathways are central to controlling cell growth and survival. LysRS works closely with mTOR a central protein that senses nutrient availability and cellular energy influencing the synthesis of proteins in response to cellular conditions. The proper functioning of these pathways is essential for cellular adaptation and responsiveness.

Lysyl-tRNA synthetase plays a significant role. Diseases like Charcot-Marie-Tooth disease and cancer are associated with LysRS mutations and dysregulation. In particular Charcot-Marie-Tooth disease showcases the involvement of LysRS through dysfunctional protein synthesis affecting peripheral nerves. LysRS's connection to cancer involves its interaction with proteins like mTOR which can influence cancer progression through altered cell growth pathways. Understanding LysRS's role provides insights into the mechanisms behind these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed : 18029264, PubMed : 18272479, PubMed : 9278442). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed : 15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed : 14975237, PubMed : 19524539, PubMed : 23159739, PubMed : 5338216).. (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
See full target information KARS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 294:4775-4783 PubMed30733335

2019

Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zhoufei Hei,Siqi Wu,Zaizhou Liu,Jing Wang,Pengfei Fang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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