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AB248725

Anti-LysRS antibody [EPR7920] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LysRS antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KARS, KIAA0070, KARS1, Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7920] - BSA and Azide free (AB248725)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7920] - BSA and Azide free (AB248725)

This data was developed using ab134912, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of LysRs in paraffin embedded Human breast carcinoma tissue, using ab134912 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7920] - BSA and Azide free (AB248725)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7920] - BSA and Azide free (AB248725)

This data was developed using ab134912, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of LysRs in HeLa cells, using ab134912 at a 1/250 dilution.

Western blot - Anti-LysRS antibody [EPR7920] - BSA and Azide free (AB248725)
  • WB

Unknown

Western blot - Anti-LysRS antibody [EPR7920] - BSA and Azide free (AB248725)

This data was developed using ab134912, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LysRS antibody [EPR7920] (<a href='/en-us/products/primary-antibodies/lysrs-antibody-epr7920-ab134912'>ab134912</a>) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7920

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248725 is the carrier-free version of ab134912.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysine-tRNA ligase also known as LysRS plays a mechanical role in the translation process by facilitating the attachment of lysine to its corresponding tRNA a critical step for protein synthesis. LysRS is a class II aminoacyl-tRNA synthetase that contributes to the accuracy of genetic code translation. It is expressed in almost all cells as it is essential for protein production. The molecular weight of LysRS is approximately 68 kDa which reflects the complexity of its function and structure.
Biological function summary

Lysyl-tRNA synthetase participates in more than just translation. It is also involved in signal transduction pathways due to its ability to interact with other proteins. LysRS forms a multi-synthetase complex which includes several other aminoacyl-tRNA synthetases and auxiliary proteins. Through this complex it influences functions like gene expression and immune responses. The activity of LysRS outside of its synthetase function shows its versatility within cellular processes.

Pathways

Lysyl-tRNA synthetase is involved in critical pathways like mTOR signaling and aminoacyl-tRNA biosynthesis. These pathways are central to controlling cell growth and survival. LysRS works closely with mTOR a central protein that senses nutrient availability and cellular energy influencing the synthesis of proteins in response to cellular conditions. The proper functioning of these pathways is essential for cellular adaptation and responsiveness.

Lysyl-tRNA synthetase plays a significant role. Diseases like Charcot-Marie-Tooth disease and cancer are associated with LysRS mutations and dysregulation. In particular Charcot-Marie-Tooth disease showcases the involvement of LysRS through dysfunctional protein synthesis affecting peripheral nerves. LysRS's connection to cancer involves its interaction with proteins like mTOR which can influence cancer progression through altered cell growth pathways. Understanding LysRS's role provides insights into the mechanisms behind these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed : 18029264, PubMed : 18272479, PubMed : 9278442). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed : 15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed : 14975237, PubMed : 19524539, PubMed : 23159739, PubMed : 5338216).. (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
See full target information KARS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4467 PubMed37491377

2023

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhong,Xujia Wu,Yixin Gao,Junju Chen,Maolei Zhang,Huangkai Zhou,Jia Yang,Feizhe Xiao,Xuesong Yang,Nunu Huang,Haoyue Qi,Xiuxing Wang,Fan Bai,Yu Shi,Nu Zhang
View all publications
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Product promise

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