Anti-LysRS antibody [EPR7921] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)

This data was developed using ab129080, the same antibody clone in a different buffer formulation.

ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)

This data was developed using ab129080, the same antibody clone in a different buffer formulation.

ab129080, at a dilution of 1/50, staining LysRS in paraffin embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)

This data was developed using ab129080, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LysRS antibody [EPR7921] (<a href='/en-us/products/primary-antibodies/lysrs-antibody-epr7921-ab129080'>ab129080</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 68 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.