Rabbit Recombinant Monoclonal LysRS antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--western-blot-img99475.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)")
![Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--immunocytochemistry-immunofluorescence-img100283.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58277.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)")
![OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--oi-rd-scanning-img82269.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (AB248286)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted |
| Rat | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:18029264, PubMed:18272479, PubMed:9278442). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:14975237, PubMed:19524539, PubMed:23159739, PubMed:5338216). (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Alternative names
KARS, KIAA0070, KARS1, Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS
Recommended products
Rabbit Recombinant Monoclonal LysRS antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7921
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.44 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248286 is the carrier-free version of Anti-LysRS antibody [EPR7921] ab129080.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lysine-tRNA ligase also known as LysRS plays a mechanical role in the translation process by facilitating the attachment of lysine to its corresponding tRNA a critical step for protein synthesis. LysRS is a class II aminoacyl-tRNA synthetase that contributes to the accuracy of genetic code translation. It is expressed in almost all cells as it is essential for protein production. The molecular weight of LysRS is approximately 68 kDa which reflects the complexity of its function and structure.
- Biological function summary
Lysyl-tRNA synthetase participates in more than just translation. It is also involved in signal transduction pathways due to its ability to interact with other proteins. LysRS forms a multi-synthetase complex which includes several other aminoacyl-tRNA synthetases and auxiliary proteins. Through this complex it influences functions like gene expression and immune responses. The activity of LysRS outside of its synthetase function shows its versatility within cellular processes.
- Pathways
Lysyl-tRNA synthetase is involved in critical pathways like mTOR signaling and aminoacyl-tRNA biosynthesis. These pathways are central to controlling cell growth and survival. LysRS works closely with mTOR a central protein that senses nutrient availability and cellular energy influencing the synthesis of proteins in response to cellular conditions. The proper functioning of these pathways is essential for cellular adaptation and responsiveness.
- Associated diseases and disorders
Lysyl-tRNA synthetase plays a significant role. Diseases like Charcot-Marie-Tooth disease and cancer are associated with LysRS mutations and dysregulation. In particular Charcot-Marie-Tooth disease showcases the involvement of LysRS through dysfunctional protein synthesis affecting peripheral nerves. LysRS's connection to cancer involves its interaction with proteins like mTOR which can influence cancer progression through altered cell growth pathways. Understanding LysRS's role provides insights into the mechanisms behind these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--western-blot-img99475.jpg "Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)")
Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
This data was developed using Anti-LysRS antibody [EPR7921] ab129080, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-LysRS antibody [EPR7921] (Anti-LysRS antibody [EPR7921] ab129080) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Lane 5: Caco-2 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--immunocytochemistry-immunofluorescence-img100283.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)")
Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
This data was developed using Anti-LysRS antibody [EPR7921] ab129080, the same antibody clone in a different buffer formulation.ICC/IF image of Anti-LysRS antibody [EPR7921] ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Anti-LysRS antibody [EPR7921] ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58277.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
This data was developed using Anti-LysRS antibody [EPR7921] ab129080, the same antibody clone in a different buffer formulation.Anti-LysRS antibody [EPR7921] ab129080, at a dilution of 1/50, staining LysRS in paraffin embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286), expandable thumbnail](https://content.abcam.com/products/images/lysrs-antibody-epr7921-bsa-and-azide-free-ab248286--oi-rd-scanning-img82269.jpg "OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)")
OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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