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AB118121

Anti-LYST antibody [3H1]

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(1 Publication)

Mouse Monoclonal LYST antibody. Carrier free. Suitable for sELISA and reacts with Recombinant full length protein, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LYST aa 1-150.

View Alternative Names

CHS, CHS1, LYST, Lysosomal-trafficking regulator, Beige homolog

1 Images
Sandwich ELISA - Anti-LYST antibody [3H1] (AB118121)
  • sELISA

Unknown

Sandwich ELISA - Anti-LYST antibody [3H1] (AB118121)

Detection limit for recombinant tagged LYST is 0.03 ng/ml as a capture antibody.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3H1

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

Recombinant Fragment Protein within Human LYST aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q99698

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Recombinant full length protein": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LYST protein also known as Lysosomal Trafficking Regulator or Beige is involved in intracellular transport processes. With an approximate molecular mass of 429 kDa it plays a role in the trafficking of lysosomal contents. Expressed in various tissues it particularly affects those with a high demand for lysosomal activity such as immune system cells. As part of its mechanical function LYST regulates the morphology of lysosomes and endosomes by facilitating fusion and movement of these vesicles.
Biological function summary

LYST directly influences the cellular degradation and recycling process. The protein does not act alone but functions as part of a larger cellular machinery that maintains lysosomal homeostasis. Its role is particularly important in immune cell function where it ensures proper lysosomal delivery and secretion especially in cytotoxic T cells and natural killer cells. These activities are important for granulocyte and lymphocyte regulation ensuring a proper response to infections and cellular stress.

Pathways

The protein LYST is integral in pathways related to vesicular transport such as the endocytic and autophagic pathways. It has a well-documented interaction with proteins such as Rab7 which is important for endosome-to-lysosome trafficking. Additionally LYST interacts with components of the cytoskeleton which further influences lysosome positioning within the cell therefore supporting its central role in cellular logistic pathways.

Mutations or functional disruptions of the LYST protein are linked to Chediak-Higashi Syndrome a rare genetic disorder characterized by immunodeficiency bleeding issues and neurological problems. This condition demonstrates a connection between LYST and the immune response as well as interactions with proteins like Perforin which are also involved in immune functions. Moreover LYST alterations might contribute to pigmentary anomalies in certain skin disorders due to its involvement in melanosome biogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes (PubMed : 11984006, PubMed : 25216107). Might regulate trafficking of effectors involved in exocytosis (PubMed : 25425525). In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules (PubMed : 26478006). In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes (By similarity). In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways (PubMed : 27881733).
See full target information LYST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 204:3008-3018 PubMed32303555

2020

CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjie Sheng,Ziyang Wang,Chen Yao,Hui-Ming Chen,Guangyan Kan,Dan Wang,Hongyuan Chen,Shuai Chen
View all publications
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Product promise

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