Anti-LYVE1 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB10278)

ab10278 (2µg/ml) staining LYVE1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is lymphatic endothelium staining of lymphatic ducts where blood vessel endothelium and smooth muscle is wholly negative.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (AB10278)

Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-LYVE1 antibody (AB10278)

Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.

• IHC-Fr

AbReview7451****

Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (AB10278)

Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1 : 500), for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.

This image is courtesy of an Abreview submitted by Dr Vyacheslav Ogay

• WB

Unknown

Western blot - Anti-LYVE1 antibody (AB10278)

LYVE-1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-LYVE1 antibody (ab10278) at 1 µg/mL

Lane 1:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 35 kDa

Observed band size: 22 kDa,37 kDa,55 kDa

true

Exposure time: 4min

• WB

Supplier Data

Western blot - Anti-LYVE1 antibody (AB10278)

All lanes:

Western blot - Anti-LYVE1 antibody (ab10278)

Lane 1:

Recombinant Human LYVE1 protein (<a href='/en-us/products/unavailable/recombinant-human-lyve1-protein-ab54341'>ab54341</a>)

Lane 2:

Recombinant Mouse LYVE1 protein (<a href='/en-us/products/unavailable/recombinant-mouse-lyve1-protein-ab54342'>ab54342</a>)

Predicted band size: 35 kDa

false