Rabbit Polyclonal LYVE1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Human, Recombinant full length protein - Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LYVE1 aa 1-250.
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- Skin health and disease 3:e2452023Inhibition of vascular endothelial growth factor-A downregulates angiogenesis in psoriasis: A pilot study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Luengas-Martinez,Dina Ismail,Ralf Paus,Helen S YoungPubMed 37799359
- Advanced materials (Deerfield Beach, Fla.) 35:e22094762023Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co-Engineering of Blood and Lymphatic Vasculature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominic Rütsche,Monica Nanni,Simon Rüdisser,Thomas Biedermann,Marcy Zenobi-WongPubMed 36724374
- Frontiers in oncology 12:8750332022ZKSCAN5 Activates Expression by Recruiting SETD7 to Promote the Lymphangiogenesis, Tumour Growth, and Metastasis of Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingtong Li,Zhifeng Yan,Jianli Ma,Zhong Chu,Huizi Li,Jingjing Guo,Qingyuan Zhang,Hui Zhao,Ying Li,Tao WangPubMed 35600335
- Journal of tissue engineering 13:204173142210885132022Bioprinting and plastic compression of large pigmented and vascularized human dermo-epidermal skin substitutes by means of a new robotic platform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Pontiggia,Ingmar Aj Van Hengel,Agnes Klar,Dominic Rütsche,Monica Nanni,Andreas Scheidegger,Sandro Figi,Ernst Reichmann,Ueli Moehrlen,Thomas BiedermannPubMed 35495096
- Biomedicines 10:2022Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Michalak-Micka,Dominic Rütsche,Lukas Johner,Ueli Moehrlen,Thomas Biedermann,Agnes S KlarPubMed 35327478
- Nanoscale research letters 14:2282019A Dual Targeting Magnetic Nanoparticle for Human Cancer Detection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiwen Wu et. al.PubMed 31289961
- Molecular medicine reports 17:2107-21122017Clinical significance of CCBE1 expression in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Li,Zhuangzhuang Cong,Yong Qiang,Lei Xiong,Li Tang,Yu Zhang,Haiwei Wu,Jun Yi,Hua Jing,Demin Li,Yi ShenPubMed 29207117
- Journal of the American College of Surgeons 225:28-402017Neoadjuvant Locoregional Therapy and Recurrent Hepatocellular Carcinoma after Liver Transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Xu et. al.PubMed 28400300
- Angiogenesis 20:73-842016Lymphangiogenesis in rat asthma model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAigul Moldobaeva,John Jenkins,Qiong Zhong,Elizabeth M WagnerPubMed 27787629
- European journal of immunology 46:1752-72016Increased numbers of CD23(+) CD21(hi) Bin-like B cells in human reactive and rheumatoid arthritis lymph nodes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIgor I Kuzin et. al.PubMed 27105894
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LYVE1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5Y7
Consider this alternative
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 6-30 µg/mL | Notes Fix sections for 10 min at -20°C in MeOH. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes (PubMed:10037799). Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (PubMed:26823460).
Alternative names
CRSBP1, HAR, XLKD1, UNQ230/PRO263, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, CRSBP-1
Recommended products
Rabbit Polyclonal LYVE1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Human, Recombinant full length protein - Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LYVE1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LYVE1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5Y7
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Protein-A Chromatography (+his tag depleted).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein "LYVE1" also known as Lymphatic Vessel Endothelial Hyaluronan Receptor 1 has a molecular mass of about 60 kDa. This protein is located on the surface of lymphatic endothelial cells. Its expression occurs mainly within the lymphatic vessels serving as an important marker for identifying lymphatic endothelium. LYVE1 facilitates the transportation of hyaluronan an important glycosaminoglycan through lymphatic vessels playing a critical role in the maintenance of extracellular matrix and tissue fluid homeostasis.
- Biological function summary
LYVE1 plays a role in lymphatic system regulation and immune cell trafficking. It forms part of a network that binds hyaluronan contributing to the regulation of cell migration and adhesion. This protein interacts with molecules like CD44 and other receptors which also participate in hyaluronan binding. LYVE1's function in maintaining the balance of hyaluronan within tissues is central affecting not only the lymphatic system but also immune responses.
- Pathways
LYVE1 engages in both the hyaluronan metabolism and lymphangiogenesis pathways. Its interaction with CD44 plays a significant role in the transport and uptake of hyaluronan influencing cellular processes such as proliferation and motility. These interactions link LYVE1 to pathways controlling fluid balance and immune surveillance marking it as essential for maintaining healthy lymphatic and immune function.
- Associated diseases and disorders
LYVE1 shows a connection to lymphatic-related conditions such as lymphedema and cancer metastasis. Alterations or dysregulation of LYVE1 expression can affect lymphatic function potentially leading to the accumulation of interstitial fluid as seen in lymphedema. In cancer increased LYVE1 expression can relate to tumor metastasis where it functions alongside proteins like VEGFR-3 in promoting lymphatic vessel growth and facilitating cancer cell spread.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab10278)
Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.
Flow Cytometry - Anti-LYVE1 antibody (ab10278)
Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab10278)
ab10278 (2µg/ml) staining LYVE1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is lymphatic endothelium staining of lymphatic ducts where blood vessel endothelium and smooth muscle is wholly negative.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-LYVE1 antibody (ab10278)
LYVE-1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-LYVE1 antibody (ab10278) at 1 µg/mL
Lane 1: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 22 kDa, 37 kDa, 55 kDa
Exposure time: 4min
Western blot - Anti-LYVE1 antibody (ab10278)
This image is courtesy of a customer review submitted by Dr Vyacheslav OgayImmunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab10278)
Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1:500), for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.
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