AB36993

Anti-LYVE1 antibody

Name: Anti-LYVE1 antibody (ab36993) | Abcam
Brand: Abcam Limited
SKU: AB36993
Price: 450 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(23 Publications)

Anti-LYVE1 antibody (ab36993) is a rabbit polyclonal antibody detecting LYVE1 in Western Blot, Flow Cytometry, IHC-P, IHC-Fr. Suitable for Dog, Human,.

- Over 10 publications
- Trusted since 2006

View Alternative Names

CRSBP1, HAR, XLKD1, UNQ230/PRO263, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, CRSBP-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB36993)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB36993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.

Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (AB36993)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (AB36993)

The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB36993)

IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.

Flow Cytometry - Anti-LYVE1 antibody (AB36993)

Flow Cyt

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Flow Cytometry - Anti-LYVE1 antibody (AB36993)

Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB36993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB36993)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.

Supplier Data

Western blot - Anti-LYVE1 antibody (AB36993)

All lanes:

Western blot - Anti-LYVE1 antibody (ab36993)

Lane 1:

Recombinant Human LYVE1 protein (<a href='/en-us/products/unavailable/recombinant-human-lyve1-protein-ab54341'>ab54341</a>)

Lane 2:

Recombinant Mouse LYVE1 protein (<a href='/en-us/products/unavailable/recombinant-mouse-lyve1-protein-ab54342'>ab54342</a>)

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Dog, Human

Applications

WB, Flow Cyt, IHC-Fr, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LYVE1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5Y7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3-10 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6-30 µg/mL", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "6-30 µg/mL", "IHCFr-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6-30 µg/mL", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-LYVE1 antibody (ab36993) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Dog, Human, samples.

What is the molecular weight of LYVE1?
Anti-LYVE1 (ab36993) specifically detects a band for LYVE1 (UniProt: Q9Y5Y7) at a molecular weight of 35kDa.

Trusted by the scientific community
Anti-LYVE1 (ab36993) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in water

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein "LYVE1" also known as Lymphatic Vessel Endothelial Hyaluronan Receptor 1 has a molecular mass of about 60 kDa. This protein is located on the surface of lymphatic endothelial cells. Its expression occurs mainly within the lymphatic vessels serving as an important marker for identifying lymphatic endothelium. LYVE1 facilitates the transportation of hyaluronan an important glycosaminoglycan through lymphatic vessels playing a critical role in the maintenance of extracellular matrix and tissue fluid homeostasis.

Biological function summary

LYVE1 plays a role in lymphatic system regulation and immune cell trafficking. It forms part of a network that binds hyaluronan contributing to the regulation of cell migration and adhesion. This protein interacts with molecules like CD44 and other receptors which also participate in hyaluronan binding. LYVE1's function in maintaining the balance of hyaluronan within tissues is central affecting not only the lymphatic system but also immune responses.

Pathways

LYVE1 engages in both the hyaluronan metabolism and lymphangiogenesis pathways. Its interaction with CD44 plays a significant role in the transport and uptake of hyaluronan influencing cellular processes such as proliferation and motility. These interactions link LYVE1 to pathways controlling fluid balance and immune surveillance marking it as essential for maintaining healthy lymphatic and immune function.

LYVE1 shows a connection to lymphatic-related conditions such as lymphedema and cancer metastasis. Alterations or dysregulation of LYVE1 expression can affect lymphatic function potentially leading to the accumulation of interstitial fluid as seen in lymphedema. In cancer increased LYVE1 expression can relate to tumor metastasis where it functions alongside proteins like VEGFR-3 in promoting lymphatic vessel growth and facilitating cancer cell spread.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes (PubMed : 10037799). Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (PubMed : 26823460).

See full target information LYVE1

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 13:5 PubMed39875968

2025

Steatohepatitis-induced vascular niche alterations promote melanoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Hoffmann,Julia Schüler,Bianca Dietsch,Sina Wietje Kürschner-Zacharias,Carsten Sticht,Felix A Trogisch,Maren Schreitmüller,Tinja Baljkas,Kai Schledzewski,Manuel Reinhart,Sebastian A Wohlfeil,Manuel Winkler,Christian David Schmid,Joerg Heineke,Cyrill Géraud,Sergij Goerdt,Philipp-Sebastian Reiners-Koch,Victor Olsavszky

Scientific reports 14:13831 PubMed38879647

2024

A novel efficient strategy to generate liver sinusoidal endothelial cells from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shang-Ping Tian,Jian-Yun Ge,Yu-Mu Song,Xiao-Qing Yu,Wen-Hao Chen,Yu-Ying Chen,Di Ye,Yun-Wen Zheng

JHEP reports : innovation in hepatology 6:101023 PubMed38681862

2024

Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver.

Applications

Unspecified application

Species

Unspecified reactive species

Kiryu K Yap,Jan Schröder,Yi-Wen Gerrand,Aleksandar Dobric,Anne M Kong,Adrian M Fox,Brett Knowles,Simon W Banting,Andrew G Elefanty,Eduoard G Stanley,George C Yeoh,Glen P Lockwood,Victoria C Cogger,Wayne A Morrison,Jose M Polo,Geraldine M Mitchell

Proceedings of the National Academy of Sciences of the United States of America 120:e2308941120 PubMed37782785

2023

A 3D biomimetic model of lymphatics reveals cell-cell junction tightening and lymphedema via a cytokine-induced ROCK2/JAM-A complex.

Applications

Unspecified application

Species

Unspecified reactive species

Esak Lee,Siu-Lung Chan,Yang Lee,William J Polacheck,Sukyoung Kwak,Aiyun Wen,Duc-Huy T Nguyen,Matthew L Kutys,Stella Alimperti,Anna M Kolarzyk,Tae Joon Kwak,Jeroen Eyckmans,Diane R Bielenberg,Hong Chen,Christopher S Chen

Immunity 56:2152-2171.e13 PubMed37582369

2023

Human microglia maturation is underpinned by specific gene regulatory networks.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Z Han,Rick Z Li,Emily Hansen,Samantha Trescott,Bethany R Fixsen,Celina T Nguyen,Cristina M Mora,Nathanael J Spann,Hunter R Bennett,Olivier Poirion,Justin Buchanan,Anna S Warden,Bing Xia,Johannes C M Schlachetzki,Martina P Pasillas,Sebastian Preissl,Allen Wang,Carolyn O'Connor,Shreya Shriram,Roy Kim,Danielle Schafer,Gabriela Ramirez,Jean Challacombe,Samuel A Anavim,Avalon Johnson,Mihir Gupta,Ian A Glass,Michael L Levy,Sharona Ben Haim,David D Gonda,Louise Laurent,Jennifer F Hughes,David C Page,Mathew Blurton-Jones,Christopher K Glass,Nicole G Coufal

Biology 12: PubMed37237550

2023

Characterization and Quantitation of the Tumor Microenvironment of Uveal Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Goesmann,Nasrin Refaian,Jacobus J Bosch,Ludwig M Heindl

Frontiers in bioengineering and biotechnology 11:1173169 PubMed37214283

2023

Low-intensity pulsed ultrasound reduces lymphedema by regulating macrophage polarization and enhancing microcirculation.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Liu,Jia Li,Yu Bian,Xiaojie Zhang,Xiaojun Cai,Yuanyi Zheng

Cell reports 39:110621 PubMed35385743

2022

Therapeutic correction of hemophilia A by transplantation of hPSC-derived liver sinusoidal endothelial cell progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Blair K Gage,Simone Merlin,Cristina Olgasi,Antonia Follenzi,Gordon M Keller

Developmental cell 57:112-145.e2 PubMed34936882

2021

A census of the lung: CellCards from LungMAP.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Sun,Anne-Karina Perl,Rongbo Li,Sheila M Bell,Eniko Sajti,Vladimir V Kalinichenko,Tanya V Kalin,Ravi S Misra,Hitesh Deshmukh,Geremy Clair,Jennifer Kyle,Laura E Crotty Alexander,Jorge A Masso-Silva,Joseph A Kitzmiller,Kathryn A Wikenheiser-Brokamp,Gail Deutsch,Minzhe Guo,Yina Du,Michael P Morley,Michael J Valdez,Haoze V Yu,Kang Jin,Eric E Bardes,Jarod A Zepp,Terren Neithamer,Maria C Basil,William J Zacharias,Jamie Verheyden,Randee Young,Gautam Bandyopadhyay,Sara Lin,Charles Ansong,Joshua Adkins,Nathan Salomonis,Bruce J Aronow,Yan Xu,Gloria Pryhuber,Jeff Whitsett,Edward E Morrisey

International journal of experimental pathology 102:268-278 PubMed34791715

2021

Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kullasit Chutipongpisit,V Praveen Parachuru,Lara T Friedlander,Haizal M Hussaini,Alison M Rich

View all publications

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