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Anti-LYVE1 antibody [EPR21771] (ab218535) is a rabbit monoclonal antibody that is used to detect LYVE1 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr. Suitable for Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (AB218535), expandable thumbnail
  • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (AB218535), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (AB218535), expandable thumbnail
  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (AB218535), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (AB218535), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-FrIHC-P
Mouse
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/60
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes (PubMed:10187853). Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (By similarity).

Alternative names

Crsbp1, Xlkd1, Lyve1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, CRSBP-1

Recommended products

Anti-LYVE1 antibody [EPR21771] (ab218535) is a rabbit monoclonal antibody that is used to detect LYVE1 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr. Suitable for Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21771
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-LYVE1 antibody [EPR21771] (ab218535) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Mouse samples.

What is the molecular weight of LYVE1?


Anti-LYVE1 [EPR21771] (ab218535) specifically detects a band for LYVE1 (UniProt: Q8BHC0) at a molecular weight of 35kDa.

Recommended positive controls


WB: Mouse lymph node and lung lysates; bEnd.3 whole cell lysate.IHC-P: Mouse liver, lung and colon tissues.IHC-Fr: Mouse liver and stomach tissues.Flow Cyt: bEnd.3 cells.IP: Mouse lung lysate.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR21771] also available for your convenience:
ab218535, Carrier free - Anti-LYVE1 antibody [EPR21771] - BSA and Azide free ab230377, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-LYVE1 antibody [EPR21771] ab252162, APC - APC Anti-LYVE1 antibody [EPR21771] ab252163



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein "LYVE1" also known as Lymphatic Vessel Endothelial Hyaluronan Receptor 1 has a molecular mass of about 60 kDa. This protein is located on the surface of lymphatic endothelial cells. Its expression occurs mainly within the lymphatic vessels serving as an important marker for identifying lymphatic endothelium. LYVE1 facilitates the transportation of hyaluronan an important glycosaminoglycan through lymphatic vessels playing a critical role in the maintenance of extracellular matrix and tissue fluid homeostasis.

Biological function summary

LYVE1 plays a role in lymphatic system regulation and immune cell trafficking. It forms part of a network that binds hyaluronan contributing to the regulation of cell migration and adhesion. This protein interacts with molecules like CD44 and other receptors which also participate in hyaluronan binding. LYVE1's function in maintaining the balance of hyaluronan within tissues is central affecting not only the lymphatic system but also immune responses.

Pathways

LYVE1 engages in both the hyaluronan metabolism and lymphangiogenesis pathways. Its interaction with CD44 plays a significant role in the transport and uptake of hyaluronan influencing cellular processes such as proliferation and motility. These interactions link LYVE1 to pathways controlling fluid balance and immune surveillance marking it as essential for maintaining healthy lymphatic and immune function.

Associated diseases and disorders

LYVE1 shows a connection to lymphatic-related conditions such as lymphedema and cancer metastasis. Alterations or dysregulation of LYVE1 expression can affect lymphatic function potentially leading to the accumulation of interstitial fluid as seen in lymphedema. In cancer increased LYVE1 expression can relate to tumor metastasis where it functions alongside proteins like VEGFR-3 in promoting lymphatic vessel growth and facilitating cancer cell spread.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LYVE1 with ab218535 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelial cells of mouse lung is observed. Counter stained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line) cells labeling LYVE1 with ab218535 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells.

  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling LYVE1 with ab218535 at 1/5000 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of lymph vessels in the submucosae on mouse stomach tissue section (PMID: 15705793).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)

    LYVE1 was immunoprecipitated from 0.35 mg mouse lung lysate with ab218535 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218535 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse lung lysate 10 μg (Input).

    Lane 2: ab218535 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab218535 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

    All lanes: Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelium of mouse colon (PMID: 14722766). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Exposure time : Lane 1: 3 minutes; Lane 2: 32 seconds; Lane 3: 5 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

    All lanes: Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535) at 1/1000 dilution

    Lane 1: Mouse lymph node lysate at 20 µg

    Lane 2: bEnd.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

    Lane 3: Mouse lung lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa

    Observed band size: 34-70 kDa

  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling LYVE1 with ab218535 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of sinusoid blood vessels on mouse liver tissue section (PMID: 11719431).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the endothelial surface of mouse hepatic sinusoids (PMID: 16353487; PMID: 11719431). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535), expandable thumbnail

    Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)

    Flow cytometry overlay histogram showing left, bEND.3 treated with 100ng/mL TNF-alpha for 24h and right, negative untreated bEND.3 stained with ab218535 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (ab218535) (1x 106 in 100μl at 10.0 μg/ml (1/209)) for 30min on ice.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

    Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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