Anti-LYVE1 antibody [EPR21857]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (AB219556)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human colon (PMID : 11377291) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (AB219556)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on blood sinusoids of human liver (PMID : 11719431) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (AB219556)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human tonsil (PMID : 11377291) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

Supplier Data

Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (AB219556)

LYVE1 was immunoprecipitated from 0.35 mg of human fetal lung lysate with ab219556 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab219556 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Human fetal lung lysate 10 μg (Input).
Lane 2 : ab219556 IP in human fetal lung lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219556 in human fetal lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 20 seconds.

The band observed is consistent with the soluble LYVE1 described in the literature (PMID : 29262593; PMID : 26966180; PMID : 26926389).

All lanes:

Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (ab219556)

Predicted band size: 35 kDa

Observed band size: 50-70 kDa

true

• WB

Supplier Data

Western blot - Anti-LYVE1 antibody [EPR21857] (AB219556)

Blocking and dilution buffer : 5% NFDM/TBST

Exposure times :

Lane 1 : 37 seconds

Lanes 2 & 4 : 92 seconds

Lane 3 : 6 seconds

Negative control : Human brain (PMID : 20599941).

Bands observed are consistent with full-length and soluble fragments, as described in the literature (PMID : 29262593, PMID : 26966180, PMID : 26926389).

All lanes:

Western blot - Anti-LYVE1 antibody [EPR21857] (ab219556) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 20 µg

Lane 2:

Human lymph node lysate at 20 µg

Lane 3:

Human fetal spleen lysate at 20 µg

Lane 4:

Human brain lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 35 kDa

Observed band size: 50-70 kDa

true