Anti-LYVE1 antibody [RM1008] (ab281587) is a rabbit recombinant multiclonal antibody that is used to detect LYVE1 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Western blot - Anti-LYVE1 antibody [RM1008] (AB281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--western-blot-img148564.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LYVE1 antibody [RM1008] (AB281587)")
![Western blot - Anti-LYVE1 antibody [RM1008] (AB281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--western-blot-img5719.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LYVE1 antibody [RM1008] (AB281587)")
![Flow Cytometry - Anti-LYVE1 antibody [RM1008] (AB281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--flow-cytometry-img66948.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-LYVE1 antibody [RM1008] (AB281587)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (AB281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128961.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (AB281587)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (AB281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19603.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (AB281587)")
Publications
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- Aging and disease 15:369-3892024Cancer-Associated Fibroblasts Promote Lymphatic Metastasis in Cholangiocarcinoma via the PDGF-BB/PDGFR-β Mediated Paracrine Signaling Network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Yan,Gang Xiao,Caini Yang,Qinqin Liu,Cui Lv,Xianhuan Yu,Ziyu Zhou,Shusheng Lin,Zhenhua Bai,Haoming Lin,Rui Zhang,Chao LiuPubMed 37307823
- Military Medical Research 10:72023Single-cell transcriptomic dissection of the cellular and molecular events underlying the triclosan-induced liver fibrosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Meng Bai,Fan Yang,Piao Luo,Lu-Lin Xie,Jun-Hui Chen,Yu-Dong Guan,Hong-Chao Zhou,Teng-Fei Xu,Hui-Wen Hao,Bing Chen,Jia-Hui Zhao,Cai-Ling Liang,Ling-Yun Dai,Qing-Shan Geng,Ji-Gang WangPubMed 36814339
- Cancer medicine 11:3820-38362022Implementing subtype-specific pre-clinical models of breast cancer to study pre-treatment aspirin effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan S Miller,Sonja Khan,Liam P Shiels,Sudipto Das,Alice C O' Farrell,Kate Connor,Adam Lafferty,Bruce Moran,Claudio Isella,Paul Loadman,Emer Conroy,Susan Cohrs,Roger Schibli,Robert S Kerbel,William M Gallagher,Elisabetta Marangoni,Kathleen Bennett,Darran P O' Connor,Róisín M Dwyer,Annette T ByrnePubMed 35434898
- Hepatology (Baltimore, Md.) 76:660-6752022A strategy of vascular-targeted therapy for liver fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Lin,Meng-Qi Dong,Zhi-Min Liu,Meng Xu,Zhi-Hao Huang,Hong-Juan Liu,Yi Gao,Wei-Jie ZhouPubMed 34940991
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Tested | Expected | Not recommended |
| Mouse | Not recommended | Tested | Tested | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Heat mediated antigen retrieval using Tris-EDTA buffer (pH9.0) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Target data
Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes (PubMed:10037799). Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (PubMed:26823460).
Alternative names
CRSBP1, HAR, XLKD1, UNQ230/PRO263, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, CRSBP-1
Recommended products
Anti-LYVE1 antibody [RM1008] (ab281587) is a rabbit recombinant multiclonal antibody that is used to detect LYVE1 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- RM1008
- Purification technique
- Affinity purification Protein A
- Specificity
We do not recommend this antibody for IHC-P with human samples. We recommend instead ab219556.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-LYVE1 antibody [RM1008] (ab281587) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse samples.
What is the molecular weight of LYVE1?
Anti-LYVE1 [RM1008] (ab281587) specifically detects a band for LYVE1 (UniProt: Q9Y5Y7) at a molecular weight of 35kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [RM1008] also available for your convenience:
ab281587, Carrier free - Anti-LYVE1 antibody [RM1008] - BSA and Azide free ab282748
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein "LYVE1" also known as Lymphatic Vessel Endothelial Hyaluronan Receptor 1 has a molecular mass of about 60 kDa. This protein is located on the surface of lymphatic endothelial cells. Its expression occurs mainly within the lymphatic vessels serving as an important marker for identifying lymphatic endothelium. LYVE1 facilitates the transportation of hyaluronan an important glycosaminoglycan through lymphatic vessels playing a critical role in the maintenance of extracellular matrix and tissue fluid homeostasis.
- Biological function summary
LYVE1 plays a role in lymphatic system regulation and immune cell trafficking. It forms part of a network that binds hyaluronan contributing to the regulation of cell migration and adhesion. This protein interacts with molecules like CD44 and other receptors which also participate in hyaluronan binding. LYVE1's function in maintaining the balance of hyaluronan within tissues is central affecting not only the lymphatic system but also immune responses.
- Pathways
LYVE1 engages in both the hyaluronan metabolism and lymphangiogenesis pathways. Its interaction with CD44 plays a significant role in the transport and uptake of hyaluronan influencing cellular processes such as proliferation and motility. These interactions link LYVE1 to pathways controlling fluid balance and immune surveillance marking it as essential for maintaining healthy lymphatic and immune function.
- Associated diseases and disorders
LYVE1 shows a connection to lymphatic-related conditions such as lymphedema and cancer metastasis. Alterations or dysregulation of LYVE1 expression can affect lymphatic function potentially leading to the accumulation of interstitial fluid as seen in lymphedema. In cancer increased LYVE1 expression can relate to tumor metastasis where it functions alongside proteins like VEGFR-3 in promoting lymphatic vessel growth and facilitating cancer cell spread.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--western-blot-img148564.jpg "Western blot - Anti-LYVE1 antibody [RM1008] (ab281587)")
Western blot - Anti-LYVE1 antibody [RM1008] (ab281587)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).
Exposure time: 37 secondsAll lanes: Western blot - Anti-LYVE1 antibody [RM1008] (ab281587) at 1/1000 dilution
Lane 1: Mouse lung lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: Rat lung lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 50-70 kDa
![Western blot - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--western-blot-img5719.jpg "Western blot - Anti-LYVE1 antibody [RM1008] (ab281587)")
Western blot - Anti-LYVE1 antibody [RM1008] (ab281587)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 min.
All lanes: Western blot - Anti-LYVE1 antibody [RM1008] (ab281587) at 1/1000 dilution
All lanes: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution
Predicted band size: 35 kDa
Observed band size: 50-70 kDa
![Flow Cytometry - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--flow-cytometry-img66948.jpg "Flow Cytometry - Anti-LYVE1 antibody [RM1008] (ab281587)")
Flow Cytometry - Anti-LYVE1 antibody [RM1008] (ab281587)
Flow cytometric analysis of bEnd.3 (mouse brain endothelioma) cells labelling LYVE1 with 281587 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)/Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Partial LYVE1 expression on bEnd.3 cells is reported in literature (PMID: 25282873).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128961.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling LYVE1 with ab281587 at 1/2000 dilution (0.293 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on endothelial vessels of mouse spleen. The section was incubated with ab281587 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19603.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling LYVE1 with ab281587 at 1/2000 dilution (0.293 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on blood sinusoids of mouse liver. The section was incubated with ab281587 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunoprecipitation-img3992.jpg "Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587)")
Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587)
LYVE1 was immunoprecipitated from 0.35 mg Mouse lung lysate with ab281587 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281587 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse lung lysate 10ug
Lane 2: ab281587 IP in Mouse lung lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab281587 in Mouse lung lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587)
Predicted band size: 35 kDa
Observed band size: 50-70 kDa
![Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunohistochemistry-frozen-sections-img122496.jpg "Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [RM1008] (ab281587)")
Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [RM1008] (ab281587)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling LYVE1 with ab281587 at 1/2000 dilution (0.228 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).
![Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunoprecipitation-img3992.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587)")
![Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [RM1008] (ab281587), expandable thumbnail](https://content.abcam.com/products/images/lyve1-antibody-rm1008-ab281587--immunohistochemistry-frozen-sections-img122496.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [RM1008] (ab281587)")
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