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AB236795

Anti-LZTFL1 antibody

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Rabbit Polyclonal LZTFL1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LZTFL1 aa 100 to C-terminus.

View Alternative Names

Leucine zipper transcription factor-like protein 1, LZTFL1

4 Images
Western blot - Anti-LZTFL1 antibody (AB236795)
  • WB

Supplier Data

Western blot - Anti-LZTFL1 antibody (AB236795)

All lanes:

Western blot - Anti-LZTFL1 antibody (ab236795) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 4:

U-251 MG (human brain glioma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 35 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (AB236795)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (AB236795)

Paraffin-embedded human testicular tissue stained for LZTFL1 using ab236795 at 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-LZTFL1 antibody (AB236795)
  • IP

Supplier Data

Immunoprecipitation - Anti-LZTFL1 antibody (AB236795)

LZTFL1 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236795 at 1/200 dilution.

Lane 1 : Rabbit control IgG IP in HepG2 whole cell lysate.

Lane 2 : ab236795 IP in HepG2 whole cell extract.

Lane 3 : HepG2 whole cell lysate (20 μg) Input.

For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-LZTFL1 antibody (ab236795)

Predicted band size: 35 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-LZTFL1 antibody (AB236795)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LZTFL1 antibody (AB236795)

U-251 MG (human brain glioma cell line) cells stained for LZTFL1 (green) using ab236795 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human LZTFL1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ48

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LZTFL1 also known as leucine zipper transcription factor-like 1 acts in cellular processes often relating to ciliary function. This protein possesses a mass of approximately 39 kDa and is expressed in tissues including lung kidney and reproductive tissues. The LZTFL1 protein has potential roles in modulating other proteins' activities by interacting with them given its leucine zipper domain which suggests it participates in protein-protein interactions.
Biological function summary

LZTFL1 plays a role in the regulation of the BBSome complex which is a critical component for ciliogenesis and the trafficking of proteins to cilia. LZTFL1 by modulating the function of the BBSome complex assists in maintaining ciliary membrane composition and cellular signaling processes. The protein facilitates the transport of proteins to the cilia therefore influencing important signaling pathways associated with cell development and maintenance.

Pathways

LZTFL1 involves itself notably in the Hedgehog signaling pathway and plays a part in the non-canonical Wnt signaling pathway. Its interaction with the BBSome complex links it to proteins such as BBS10 and BBS12 which are essential in these signaling pathways for their roles in cell differentiation and organ development. By engaging with these pathways LZTFL1 supports cell signaling cascades that are fundamental for proper cellular responses to external stimuli.

LZTFL1 correlates with conditions such as Bardet-Biedl syndrome and certain types of cancer. In Bardet-Biedl syndrome the dysfunction of LZTFL1 affects the BBSome complex leading to impaired ciliary function and symptoms associated with the syndrome. LZTFL1 also connects to proteins like GLI1 in cancer signaling pathways which indicates its involvement in oncogenesis through the modulation of cell proliferation and survival signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates ciliary localization of the BBSome complex. Together with the BBSome complex, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May play a role in neurite outgrowth. May have tumor suppressor function.
See full target information LZTFL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB187918

Anti-LZTFL1 antibody

primary-antibodies

lztfl1-antibody-ab187918

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