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AB314582

Anti-LZTFL1 antibody [EPR27975-2] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal LZTFL1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Leucine zipper transcription factor-like protein 1, LZTFL1

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Sandwich ELISA - Anti-LZTFL1 antibody [EPR27975-2] - BSA and Azide free (Capture) (AB314582)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-LZTFL1 antibody [EPR27975-2] - BSA and Azide free (Capture) (AB314582)

Sandwich ELISA of ab314581 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27975-2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314582 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LZTFL1. The recommended pair for sandwich ELISA is:

Capture: ab314582, Human LZTFL1 Capture Antibody (unconjugated)

Detector: ab314583, Human LZTFL1 Detector Antibody (unconjugated)

The reference range value is 39.1-2500 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LZTFL1 also known as leucine zipper transcription factor-like 1 acts in cellular processes often relating to ciliary function. This protein possesses a mass of approximately 39 kDa and is expressed in tissues including lung kidney and reproductive tissues. The LZTFL1 protein has potential roles in modulating other proteins' activities by interacting with them given its leucine zipper domain which suggests it participates in protein-protein interactions.
Biological function summary

LZTFL1 plays a role in the regulation of the BBSome complex which is a critical component for ciliogenesis and the trafficking of proteins to cilia. LZTFL1 by modulating the function of the BBSome complex assists in maintaining ciliary membrane composition and cellular signaling processes. The protein facilitates the transport of proteins to the cilia therefore influencing important signaling pathways associated with cell development and maintenance.

Pathways

LZTFL1 involves itself notably in the Hedgehog signaling pathway and plays a part in the non-canonical Wnt signaling pathway. Its interaction with the BBSome complex links it to proteins such as BBS10 and BBS12 which are essential in these signaling pathways for their roles in cell differentiation and organ development. By engaging with these pathways LZTFL1 supports cell signaling cascades that are fundamental for proper cellular responses to external stimuli.

LZTFL1 correlates with conditions such as Bardet-Biedl syndrome and certain types of cancer. In Bardet-Biedl syndrome the dysfunction of LZTFL1 affects the BBSome complex leading to impaired ciliary function and symptoms associated with the syndrome. LZTFL1 also connects to proteins like GLI1 in cancer signaling pathways which indicates its involvement in oncogenesis through the modulation of cell proliferation and survival signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates ciliary localization of the BBSome complex. Together with the BBSome complex, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May play a role in neurite outgrowth. May have tumor suppressor function.
See full target information LZTFL1
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Product promise

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For full details, please see our Terms & Conditions

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