Anti-LZTFL1 antibody [EPR27975-54]

5 product images

• 

  Immunoprecipitation - Anti-LZTFL1 antibody [EPR27975-54] (ab313634)

  LZTFL1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab313634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313634 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  Lane 2: ab313634 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313634 in HeLa whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 180 seconds

  All lanes: Immunoprecipitation - Anti-LZTFL1 antibody [EPR27975-54] (ab313634) at 1/30 dilution

  All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

  Exposure time: 180s

• 

  Western blot - Anti-LZTFL1 antibody [EPR27975-54] (ab313634)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Low expression: heart, skeletal muscle (PMID: 34023333).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: 180 seconds.

  All lanes: Western blot - Anti-LZTFL1 antibody [EPR27975-54] (ab313634) at 1/1000 dilution

  Lane 1: Mouse testis tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

  Lane 4: Rat testis tissue lysate at 20 µg

  Lane 5: Rat heart tissue lysate at 20 µg

  Lane 6: Rat skeletal muscle tissue lysate at 20 µg

  Lane 7: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

  Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 34 kDa

  Exposure time: 180s

• 

  Western blot - Anti-LZTFL1 antibody [EPR27975-54] (ab313634)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Low expression: HepG2 (PMID: 31371991).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: Lanes 1-2: 15 seconds;
  Lanes 3-5: 180 seconds.

  All lanes: Western blot - Anti-LZTFL1 antibody [EPR27975-54] (ab313634) at 1/1000 dilution

  Lane 1: MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate at 20 µg

  Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 34 kDa

• 

  Western blot - Anti-LZTFL1 antibody [EPR27975-54] (ab313634)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Low expression: spleen (PMID: 34023333).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: Lanes 1-2: 15 seconds;
  Lanes 3-5: 180 seconds.

  All lanes: Western blot - Anti-LZTFL1 antibody [EPR27975-54] (ab313634) at 1/1000 dilution

  Lane 1: Human testis tissue lysate at 20 µg

  Lane 2: Human spleen tissue lysate at 20 µg

  Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 5: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

  Secondary

  Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Lanes 3 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 34 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-LZTFL1 antibody [EPR27975-54] (ab313634)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling LZTFL1 with ab313634 at 1/50 (10.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in MOLT-4 cells.Low expression: HepG2 (PMID:31371991)Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.