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AB309477

Anti-M Cadherin antibody [EPR25401-56]

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Rabbit Recombinant Monoclonal M Cadherin antibody. Suitable for ICC/IF and reacts with Mouse samples.

View Alternative Names

CAD15_HUMAN, CCAD, CDH 14, CDH 15, CDH 3, CDHM, Cadherin 15, type 1, M cadherin (myotubule), Cadherin 3, Cadherin-14, M-cadherin, MCAD, MRD3, Muscle cadherin, Myotubule cadherin

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-M Cadherin antibody [EPR25401-56] (AB309477)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-M Cadherin antibody [EPR25401-56] (AB309477)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labelling M Cadherin with ab309477 at 1/500 (1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membranous and cytoplasmic staining in C2C12 cell line.Negative control : NIH/3T3 (PMID : 11204426)Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25401-56

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

M-Cadherin also known as CDH15 is a member of the cadherin superfamily. This calcium-dependent cell adhesion molecule is approximately 100 kilodaltons in mass. M-Cadherin is expressed mostly in muscle tissues where it plays a role in cell recognition and connection processes. The presence of this protein on the cell surface helps in forming intercellular junctions contributing to the stabilization of cell interactions which are essential for developmental and repair functions in muscle cells.
Biological function summary

M-Cadherin facilitates cell-cell adhesion which is vital for the proper alignment of muscle cells during development and repair. This protein is a critical component of the adherens junction complex. During muscle regeneration M-Cadherin helps satellite cells to adhere to existing muscle fibers promoting the fusion needed for effective muscle repair. It operates alongside other proteins including β-catenin and α-catenin which link cadherins to the actin cytoskeleton reinforcing structural integrity.

Pathways

Different cellular processes involve M-Cadherin playing a role in the Wnt signaling and Notch signaling pathways. Wnt signaling is significant in muscle development and regeneration. M-Cadherin interacts with β-catenin in this pathway balancing the regulation of cellular adhesion and signaling events. In the Notch signaling pathway M-Cadherin influences satellite cell activation and proliferation helping maintain muscle stem cell populations which are important under conditions requiring muscle repair.

M-Cadherin has implications in muscle-related conditions such as muscular dystrophy and muscle degeneration. The protein's malfunction can result in impaired cell adhesion leading to defects in muscle repair mechanisms. In muscular dystrophy M-Cadherin-related disruptions are observed often together with abnormal β-catenin interactions. These disruptions contribute to inadequate muscle regeneration and muscular weakness underlining M-Cadherin's importance in maintaining muscle health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Cadherin-15
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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB190076

Anti-P cadherin antibody - N-terminal

Western blot - Anti-P cadherin antibody - N-terminal (AB190076)

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Proteins & Peptides

AB182803

Recombinant Human M Cadherin protein

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Primary Antibodies

AB270263

Anti-Cadherin 16 antibody [CDH16/1532R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] (AB270263)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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