AB290747

Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal M Cadherin antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Cdh14, Cdh15, Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

This data was developed using ab290732, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling M Cadherin with ab290732 at 1/4000 (0.114 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on granule cells of mouse cerebellum. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

This data was developed using ab290732, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling M Cadherin with ab290732 at 1/4000 (0.114 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control : no staining on mouse cerebrum. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

This data was developed using ab290732, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labelling M Cadherin with ab290732 at 1/4000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on satellite cells of rat skeletal muscle. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

This data was developed using ab290732, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labelling M Cadherin with ab290732 at 1/4000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) as a secondary antibody. Positive staining on satellite cells of mouse skeletal muscle. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25401-63

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background.2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background.2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background.2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

M-Cadherin also known as CDH15 is a member of the cadherin superfamily. This calcium-dependent cell adhesion molecule is approximately 100 kilodaltons in mass. M-Cadherin is expressed mostly in muscle tissues where it plays a role in cell recognition and connection processes. The presence of this protein on the cell surface helps in forming intercellular junctions contributing to the stabilization of cell interactions which are essential for developmental and repair functions in muscle cells.

Biological function summary

M-Cadherin facilitates cell-cell adhesion which is vital for the proper alignment of muscle cells during development and repair. This protein is a critical component of the adherens junction complex. During muscle regeneration M-Cadherin helps satellite cells to adhere to existing muscle fibers promoting the fusion needed for effective muscle repair. It operates alongside other proteins including β-catenin and α-catenin which link cadherins to the actin cytoskeleton reinforcing structural integrity.

Pathways

Different cellular processes involve M-Cadherin playing a role in the Wnt signaling and Notch signaling pathways. Wnt signaling is significant in muscle development and regeneration. M-Cadherin interacts with β-catenin in this pathway balancing the regulation of cellular adhesion and signaling events. In the Notch signaling pathway M-Cadherin influences satellite cell activation and proliferation helping maintain muscle stem cell populations which are important under conditions requiring muscle repair.

M-Cadherin has implications in muscle-related conditions such as muscular dystrophy and muscle degeneration. The protein's malfunction can result in impaired cell adhesion leading to defects in muscle repair mechanisms. In muscular dystrophy M-Cadherin-related disruptions are observed often together with abnormal β-catenin interactions. These disruptions contribute to inadequate muscle regeneration and muscular weakness underlining M-Cadherin's importance in maintaining muscle health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.

See full target information Cdh15

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (AB290747)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise