AB9693

Anti-M-CSF antibody

Name: Anti-M-CSF antibody (ab9693) | Abcam
Brand: Abcam Limited
SKU: AB9693
Rating: 5 (1 reviews)

(1 Review)

(20 Publications)

Rabbit Polyclonal M-CSF antibody. Suitable for ELISA, WB, sELISA, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CSF1.

View Alternative Names

Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1

3 Images

AbReview19185****

Western blot - Anti-M-CSF antibody (AB9693)

Blocking Step : 5% BSA for 1 hour at 25°C

Deatection method : Odyssey for 50 seconds.

Right-hand Image : Weaker exposure to identify bands.

All lanes:

Western blot - Anti-M-CSF antibody (ab9693) at 1/500 dilution

Lane 1:

Human Kidney whole cell lysate at 15 µg

Lane 2:

Human Kidney whole cell lysate transfected with LFABP at 15 µg

Secondary

All lanes:

An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

This image is courtesy of an Abreview submitted by Valentina Marcelli

Sandwich ELISA - Anti-M-CSF antibody (AB9693)

sELISA

Unknown

Sandwich ELISA - Anti-M-CSF antibody (AB9693)

Standard curve for MCSF (Analyte : ab62015); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1μg/ml.

Unknown

Western blot - Anti-M-CSF antibody (AB9693)

All lanes:

Western blot - Anti-M-CSF antibody (ab9693) at 0.2 µg/mL

All lanes:

Western blot - Recombinant human M-CSF protein (<a href='/en-us/products/proteins-peptides/recombinant-human-m-csf-protein-ab9694'>ab9694</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 60 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

sELISA, ELISA, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CSF1.

P09603

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.1 µg/mL", "sELISA-species-notes": "To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "To detect hM-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab52864

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute with 200µl of sterile water.

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.

Biological function summary

M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

Pathways

The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

See full target information CSF1

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0264775 PubMed35226700

2022

Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Jere Kurkipuro,Igor Mierau,Thomas Wirth,Haritha Samaranayake,Wesley Smith,Hanna-Riikka Kärkkäinen,Mirka Tikkanen,Juha Yrjänheikki

Signal transduction and targeted therapy 6:10 PubMed33431788

2021

CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Hui Huang,Kun Cai,Peng-Peng Xu,Li Wang,Chuan-Xin Huang,Ying Fang,Shu Cheng,Xiao-Jian Sun,Feng Liu,Jin-Yan Huang,Meng-Meng Ji,Wei-Li Zhao

Anticancer research 39:6403-6412 PubMed31704874

2019

Plasma Levels and Tissue Expression of Selected Cytokines, Metalloproteinases and Tissue Inhibitors in Patients With Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Iwona Sidorkiewicz,Barbara Piskór,Emilia Dąbrowska,Katarzyna Guzińska-Ustymowicz,Anna Pryczynicz,Monika Zbucka-Krętowska,Sławomir Ławicki

American journal of reproductive immunology (New York, N.Y. : 1989) 83:e13191 PubMed31536655

2019

CSF1-associated decrease in endometrial macrophages may contribute to Asherman's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Liu,Jiali Wang,Guangfeng Zhao,Peipei Jiang,Minmin Song,Hailin Ding,Zhiyin Wang,Haining Lv,Yali Hu

RNA biology 16:1249-1262 PubMed31204558

2019

CircRNA_28313/miR-195a/CSF1 axis modulates osteoclast differentiation to affect OVX-induced bone absorption in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Chen,Zhengxiao Ouyang,Yi Shen,Bo Liu,Qiang Zhang,Lu Wan,Ziqing Yin,Wei Zhu,Shuai Li,Dan Peng

Cell death & disease 9:962 PubMed30237497

2018

A miR-125b/CSF1-CX3CL1/tumor-associated macrophage recruitment axis controls testicular germ cell tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Aalia Batool,Yu-Qian Wang,Xiao-Xia Hao,Su-Ren Chen,Yi-Xun Liu

European journal of oral sciences 126:439-448 PubMed30216610

2018

Study on site-specific expression of bone formation and resorption factors in human dental follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela Uribe,Pawel Plakwicz,Lena Larsson,Ewa Czochrowska,Anna Westerlund,Maria Ransjö

Nature communications 9:559 PubMed29422647

2018

Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.

Applications

Species

Unspecified reactive species

Qirui Wang,Zhenqiang He,Menggui Huang,Tianrun Liu,Yanling Wang,Haineng Xu,Hao Duan,Peihong Ma,Lin Zhang,Scott S Zamvil,Juan Hidalgo,Zhenfeng Zhang,Donald M O'Rourke,Nadia Dahmane,Steven Brem,Yonggao Mou,Yanqing Gong,Yi Fan

Molecular medicine reports 17:6045-6053 PubMed29436622

2018

Hepatocyte growth factor improves bone regeneration via the bone morphogenetic protein‑2‑mediated NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixin Zhen,Jianing Yang,Yu Wang,Yubo Li,Bin Chen,Youxin Song,Guiyun Ma,Bo Yang

Cancer letters 415:20-29 PubMed29199004

2017

Dicer reprograms stromal fibroblasts to a pro-inflammatory and tumor-promoting phenotype in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zongyuan Yang,Ping Jin,Sen Xu,Taoran Zhang,Xin Yang,Xiaoting Li,Xiao Wei,Chaoyang Sun,Gang Chen,Ding Ma,Qinglei Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-M-CSF antibody

Western blot - Anti-M-CSF antibody (AB9693)

Sandwich ELISA - Anti-M-CSF antibody (AB9693)

Western blot - Anti-M-CSF antibody (AB9693)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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