Rabbit Polyclonal M-CSF antibody. Suitable for ELISA, WB, sELISA, IHC-P, Neut and reacts with Recombinant full length protein - Human, Human, Recombinant fragment samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CSF1.
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- PloS one 17:e02647752022Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJere Kurkipuro,Igor Mierau,Thomas Wirth,Haritha Samaranayake,Wesley Smith,Hanna-Riikka Kärkkäinen,Mirka Tikkanen,Juha YrjänheikkiPubMed 35226700
- Signal transduction and targeted therapy 6:102021CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao-Hui Huang et. al.PubMed 33431788
- Anticancer research 39:6403-64122019Plasma Levels and Tissue Expression of Selected Cytokines, Metalloproteinases and Tissue Inhibitors in Patients With Cervical Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIwona Sidorkiewicz,Barbara Piskór,Emilia Dąbrowska,Katarzyna Guzińska-Ustymowicz,Anna Pryczynicz,Monika Zbucka-Krętowska,Sławomir ŁawickiPubMed 31704874
- American journal of reproductive immunology (New York, N.Y. : 1989) 83:e131912019CSF1-associated decrease in endometrial macrophages may contribute to Asherman's syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Liu,Jiali Wang,Guangfeng Zhao,Peipei Jiang,Minmin Song,Hailin Ding,Zhiyin Wang,Haining Lv,Yali HuPubMed 31536655
- RNA biology 16:1249-12622019CircRNA_28313/miR-195a/CSF1 axis modulates osteoclast differentiation to affect OVX-induced bone absorption in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Chen,Zhengxiao Ouyang,Yi Shen,Bo Liu,Qiang Zhang,Lu Wan,Ziqing Yin,Wei Zhu,Shuai Li,Dan PengPubMed 31204558
- Cell death & disease 9:9622018A miR-125b/CSF1-CX3CL1/tumor-associated macrophage recruitment axis controls testicular germ cell tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAalia Batool et. al.PubMed 30237497
- European journal of oral sciences 126:439-4482018Study on site-specific expression of bone formation and resorption factors in human dental follicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela Uribe et. al.PubMed 30216610
- Nature communications 9:5592018Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
- Molecular medicine reports 17:6045-60532018Hepatocyte growth factor improves bone regeneration via the bone morphogenetic protein‑2‑mediated NF‑κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuixin Zhen et. al.PubMed 29436622
- Cancer letters 415:20-292017Dicer reprograms stromal fibroblasts to a pro-inflammatory and tumor-promoting phenotype in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZongyuan Yang,Ping Jin,Sen Xu,Taoran Zhang,Xin Yang,Xiaoting Li,Xiao Wei,Chaoyang Sun,Gang Chen,Ding Ma,Qinglei GaoPubMed 29199004
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CSF1. Database link P09603
Consider this alternative
Reactivity data
Select an application| ELISA | WB | sELISA | IHC-P | Neut | |
|---|---|---|---|---|---|
| Human | Predicted | Tested | Tested | Expected | Expected |
| Recombinant fragment | Not recommended | Not recommended | Not recommended | Not recommended | Expected |
| Recombinant full length protein - Human | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect hM-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1 µg/mL | Notes To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 μg/ml of this antibody is required. |
Species Human | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 μg/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Alternative names
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1
Recommended products
Rabbit Polyclonal M-CSF antibody. Suitable for ELISA, WB, sELISA, IHC-P, Neut and reacts with Recombinant full length protein - Human, Human, Recombinant fragment samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CSF1.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CSF1. Database link P09603
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-M-CSF antibody [EP1179Y] ab52864
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
- Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
- Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
- Associated diseases and disorders
Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
This image is courtesy of a customer review submitted by Valentina MarcelliWestern blot - Anti-M-CSF antibody (ab9693)
Blocking Step: 5% BSA for 1 hour at 25°C
Deatection method: Odyssey for 50 seconds.
Right-hand Image: Weaker exposure to identify bands.All lanes: Western blot - Anti-M-CSF antibody (ab9693) at 1/500 dilution
Lane 1: Human Kidney whole cell lysate at 15 µg
Lane 2: Human Kidney whole cell lysate transfected with LFABP at 15 µg
SecondaryAll lanes: An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa
Western blot - Anti-M-CSF antibody (ab9693)
All lanes: Western blot - Anti-M-CSF antibody (ab9693) at 0.2 µg/mL
All lanes: Western blot - Recombinant human M-CSF protein (Recombinant human M-CSF protein ab9694) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Exposure time: 1min
Sandwich ELISA - Anti-M-CSF antibody (ab9693)
Standard curve for MCSF (Analyte: Recombinant human M-CSF protein (Active) ab62015); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1μg/ml.
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