Rabbit Polyclonal M-CSF antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications.
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- BMC cancer 21:10902021CircCDC45 promotes the malignant progression of glioblastoma by modulating the miR-485-5p/CSF-1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongcai Liu,Weimin Dai,An Wu,Yunping LiPubMed 34627193
- International journal of molecular sciences 22:2021The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlaa Sawaied,Eden Arazi,Ahmad AbuElhija,Eitan Lunenfeld,Mahmoud HuleihelPubMed 33652607
- Cellular and molecular gastroenterology and hepato :2021Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Samain et. al.PubMed 33482394
- PloS one 16:e02453402021Reno-protective effect of IL-34 inhibition on cisplatin-induced nephrotoxicity in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukihiro Wada,Masayuki Iyoda,Kei Matsumoto,Taihei Suzuki,Shohei Tachibana,Nobuhiro Kanazawa,Hirokazu HondaPubMed 33428678
- Cancer immunology research 8:131-1452019Myeloid Cells Orchestrate Systemic Immunosuppression, Impairing the Efficacy of Immunotherapy against HPV Cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriele Galliverti,Stephan Wullschleger,Mélanie Tichet,Dhaarini Murugan,Nadine Zangger,Wesley Horton,Alan J Korman,Lisa M Coussens,Melody A Swartz,Douglas HanahanPubMed 31771984
- IUBMB life 71:1916-19282019MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongxuan Mao,Yihai Zhu,Weimin Hao,Chao Chu,Hongmin SuPubMed 31317664
- Immunity 49:275-287.e52018Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyung-Geun Moon et. al.PubMed 30054206
- Arteriosclerosis, thrombosis, and vascular biology 37:312-3152017Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenske J de Jong,Nicole Paulin,Patricia Lemnitzer,Joana R Viola,Carla Winter,Bartolo Ferraro,Jochen Grommes,Christian Weber,Chris Reutelingsperger,Maik Drechsler,Oliver SoehnleinPubMed 28062503
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Macaque monkey, Gorilla | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Macaque monkey, Gorilla | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Alternative names
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1
Recommended products
Rabbit Polyclonal M-CSF antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
- Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
- Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
- Associated diseases and disorders
Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody (ab99178)
IHC image of MCSF staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99178, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-M-CSF antibody (ab99178)
Blocking buffer: 2% BSA
Gel type: MOPSAll lanes: Western blot - Anti-M-CSF antibody (ab99178) at 1 µg/mL
All lanes: Mouse Spleen lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: 8min
Western blot - Anti-M-CSF antibody (ab99178)
MCSF contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-M-CSF antibody (ab99178) at 1 µg/mL
Lane 1: Bone Marrow (Human) Tissue Lysate - adult normal tissue at 10 µg
Lane 2: Human spleen tissue lysate - total protein (ab29699) at 10 µg
Lane 3: Human thymus tissue lysate - total protein (ab30146) at 10 µg
Lane 4: Tonsil (Human) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 170 kDa, 52 kDa, 70 kDa, 98 kDa
Exposure time: 8min
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody (ab99178)
ab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.
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