Rabbit Recombinant Monoclonal M-CSF antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human samples. Cited in 25 publications.
Images
![Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (AB52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunoprecipitation-img3563.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (AB52864)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (AB52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42586.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (AB52864)")
![Western blot - Anti-M-CSF antibody [EP1179Y] (AB52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--western-blot-img98973.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-M-CSF antibody [EP1179Y] (AB52864)")
![Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (AB52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--flow-cytometry-intracellular-img136890.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (AB52864)")
![Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (AB52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunocytochemistry-immunofluorescence-img95117.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (AB52864)")
Publications
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- Reproductive sciences (Thousand Oaks, Calif.) 30:2457-24672023Genome-Wide Gene Expression Profiling Reveals the Direct Effect of Dienogest on Ovarian Endometriotic Stromal Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Honda,Norihisa Nishimichi,Mayumi Kaneko,Michinori Yamashita,Yumiko Akimoto,Hirotoshi Tanimoto,Mitsue Teramoto,Hideki Teramoto,Yasuyuki YokosakiPubMed 36752987
- Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:1918-19252021Neoplastic synovial lining cells that coexpress podoplanin and CD90 overproduce CSF-1, driving tenosynovial giant cell tumor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew C Chandler,Mohamed Yakoub,Tomohiro Fujiwara,Laura T Donlin,Paul Edward Purdue,John H HealeyPubMed 34855235
- Bone research 9:432021Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJungho Back,Minh Nam Nguyen,Lu Li,Saelim Lee,Inkyu Lee,Fancheng Chen,Lauren Gillinov,Yeon-Ho Chung,Kareme D Alder,Hyuk-Kwon Kwon,Kristin E Yu,Christopher M Dussik,Zichen Hao,Michael J Flores,Yoseph Kim,Izuchukwu K Ibe,Alana M Munger,Sung Wook Seo,Francis Y LeePubMed 34588427
- Molecular cancer research : MCR 19:1085-10952021Neoadjuvant Chemotherapy Induces IL34 Signaling and Promotes Chemoresistance via Tumor-Associated Macrophage Polarization in Esophageal Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShotaro Nakajima,Kosaku Mimura,Katsuharu Saito,Aung Kyi Thar Min,Eisei Endo,Leo Yamada,Koji Kase,Naoto Yamauchi,Takuro Matsumoto,Hiroshi Nakano,Yasuyuki Kanke,Hirokazu Okayama,Motonobu Saito,Prajwal Neupane,Zenichiro Saze,Yohei Watanabe,Hiroyuki Hanayama,Suguru Hayase,Akinao Kaneta,Tomoyuki Momma,Shinji Ohki,Hiromasa Ohira,Koji KonoPubMed 33674443
- Aging 12:12684-127022020Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuyu Lin et. al.PubMed 32629426
- Oncology letters 20:1835-18452020CSF1R methylation is a key regulatory mechanism of tumor-associated macrophages in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Cui et. al.PubMed 32724427
- Journal of hematology & oncology 13:622020Oct4 promotes M2 macrophage polarization through upregulation of macrophage colony-stimulating factor in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Sing Lu et. al.PubMed 32487125
- EMBO reports 21:e487802020High-grade mesenchymal pancreatic ductal adenocarcinoma drives stromal deactivation through CSF-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Steins et. al.PubMed 32173982
- Virchows Archiv : an international journal of path 474:375-3812018M-CSF and IL-34 expression as indicators for growth in sporadic vestibular schwannoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesW M de Vries et. al.PubMed 30580386
- Journal of clinical and experimental hematopathology : JCEH 58:152-1602018Potential anti-lymphoma effect of M-CSFR inhibitor in adult T-cell leukemia/lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshihiro Komohara,Osamu Noyori,Yoichi Saito,Hiroto Takeya,Muhammad Baghdadi,Fumihito Kitagawa,Naoki Hama,Kozo Ishikawa,Yutaka Okuno,Kisato Nosaka,Ken-Ichiro Seino,Masao Matsuoka,Shinya SuzuPubMed 30541986
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted | Predicted | Predicted |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/50 - 1/100 dilution. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Chimpanzee | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes For unpurified use at 1/50 dilution. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Chimpanzee | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/2000 | Notes This antibody fails to detect endogenous natural samples in WB. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes For unpurified use at 1/250 - 1/500 dilution. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Chimpanzee | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/80 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Chimpanzee | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Alternative names
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1
Recommended products
Rabbit Recombinant Monoclonal M-CSF antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human samples. Cited in 25 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1179Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody fails to detect endogenous natural samples in WB.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.
- Biological function summary
M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.
- Pathways
The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.
- Associated diseases and disorders
Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunoprecipitation-img3563.jpg "Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
ab52864 (purified) at 1:40 dilution (2μg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10μg
Lane 2 (+): ab52864 & THP-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52864 in THP-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
Predicted band size: 60 kDa
Observed band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42586.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
![Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--western-blot-img98973.jpg "Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
Blocking buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/2000 dilution
All lanes: Human M-CSF (aa 33-190) recombinant protein at 0.015 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 19 kDa
![Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--flow-cytometry-intracellular-img136890.jpg "Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)
Intracellular Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1/80 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr®488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunocytochemistry-immunofluorescence-img95117.jpg "Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)
Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123726.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864 (1/50 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864), expandable thumbnail](https://content.abcam.com/products/images/m-csf-antibody-ep1179y-ab52864--western-blot-img159480.gif "Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)")
Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
All lanes: Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution
All lanes: recombinant MCSF at 0.005 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 20 kDa
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