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Rabbit Recombinant Monoclonal M-CSF antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment samples.

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Images

Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (AB232165), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (AB232165), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (AB232165), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (AB232165), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (AB232165), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
Tested
Recombinant fragment
Not recommended
Not recommended
Expected
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Expected
Expected

Species

Recombinant fragment, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

    4 products for Alternative Version

      Target data

      Function

      Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

      Alternative names

      Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1

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      Rabbit Recombinant Monoclonal M-CSF antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment samples.

      Alternative names

      Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EP1179Y

      Purification technique

      Affinity purification Protein A

      Specificity

      This antibody fails to detect endogenous natural samples in WB.

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab232165 is the carrier-free version of Anti-M-CSF antibody [EP1179Y] ab52864.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.

      Biological function summary

      M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

      Pathways

      The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

      Associated diseases and disorders

      Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165), expandable thumbnail

        Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

        Anti-M-CSF antibody [EP1179Y] ab52864 (purified) at 1:40 dilution (2μg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
        Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10μg
        Lane 2 (+): Anti-M-CSF antibody [EP1179Y] ab52864 & THP-1 whole cell lysate
        Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-M-CSF antibody [EP1179Y] ab52864 in THP-1 whole cell lysate
        For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
        Blocking and diluting buffer: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-M-CSF antibody [EP1179Y] ab52864).

        All lanes: Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (Anti-M-CSF antibody [EP1179Y] ab52864)

        Predicted band size: 60 kDa

        Observed band size: 60 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified Anti-M-CSF antibody [EP1179Y] ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-M-CSF antibody [EP1179Y] ab52864).

      • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

        Intracellular Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified Anti-M-CSF antibody [EP1179Y] ab52864 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-M-CSF antibody [EP1179Y] ab52864).

      • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

        Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified Anti-M-CSF antibody [EP1179Y] ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-M-CSF antibody [EP1179Y] ab52864).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] - BSA and Azide free (ab232165)

        Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with Anti-M-CSF antibody [EP1179Y] ab52864 (1/50 dilution) in immunohistochemical analysis.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-M-CSF antibody [EP1179Y] ab52864).

        Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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