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AB235228

Anti-M13 antibody

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Rabbit Polyclonal M13 antibody. Suitable for ELISA and reacts with Synthetic peptide samples.

View Alternative Names

Bacteriophage M13, Coliphage M13, Enterobacteria phage M13, M13 virus, Phage M13

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ELISA - Anti-M13 antibody (AB235228)
  • ELISA

Supplier Data

ELISA - Anti-M13 antibody (AB235228)

ELISA - Anti-M13 antibody (ab235228).

ab235228 used at 5 μg/mL.

BSA-M13 peptide Coated at 0.1 μg/well.

Secondary used is a goat anti-rabbit IgG [H&L] HRP at a 1/30,000 dilution.

Buffer : 50mM NaCHO3 pH 9.5.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'M13' also known as M13 bacteriophage is a filamentous bacteriophage with a mass of approximately 2 MDa. This bacteriophage infects Escherichia coli bacteria and is characterized by a cylindrical shape with helical symmetry. The M13 phage expresses a protein coat made up of thousands of coat proteins including major coat protein g8p and minor proteins like g3p at its ends. These proteins interact with the bacterial surface for infection.
Biological function summary

M13 phage serves as a tool in phage display technology facilitating the identification of peptide-protein interactions. It does not actively participate in cellular processes but can be part of complex interactions in biotechnological applications. In contrast to lytic phages M13 allows for the continued survival of its host leading to its utilization in non-destructive recombinant DNA methodologies.

Pathways

M13 phage does not integrate into traditional metabolic or signaling pathways of the bacterial host. Instead it exploits the host's machinery for its replication and assembly. Not directly related to human biological pathways its use in phage display allows exploring interactions involving proteins such as antibodies or enzymes including the anti M13 HRP.

M13 phage does not directly relate to human diseases but it is essential in diagnostics and therapeutic antibody development. Researchers use phage display libraries to identify antibodies against targets involved in cancer and autoimmune diseases. The interaction between M13 and the Fc region of antibodies is exploited aiding in the precise selection of antibody fragments for therapeutic applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information M13
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Product promise

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