AB9225

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

Name: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) | Abcam
Brand: Abcam Limited
SKU: AB9225
Price: 410 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(6 Publications)

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody detecting M13 Bacteriophage Coat Protein g8p in Western Blot, Flow Cytometry. Suitable for Enterobacteria phage M13.

- Trusted since 2002

View Alternative Names

Capsid protein G8P, Coat protein B, Gene 8 protein, M13 procoat, Major coat protein, pVIII, G8P, VIII, G8P, VIII, Coat protein B, Capsid protein G8P, Major coat protein

1 Images

AbReview20684****

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225)

Primary antibody incubated for 16 hours at 4°C.
Blocking step performed using 5% milk for 1 hour at room temperature.

All lanes:

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution

All lanes:

whole cell lysate prepared from XL10Gold cells at 50 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/2000 dilution

Predicted band size: 8 kDa

Observed band size: 6 kDa

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Image courtesy of an anonymous Abreview.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RL-ph2

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Enterobacteria phage M13

Applications

WB, ICC, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Enterobacteria phage M13 Capsid protein G8P.

P69541

Specificity

RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Enterobacteria phage M13": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/25 - 1/200", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Recombinant full length protein - Enterobacteria phage M13": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Enterobacteria phage M13 samples.

What is the molecular weight of M13 Bacteriophage Coat Protein g8p?
Anti-M13 Bacteriophage Coat Protein g8p [RL-ph2] (ab9225) specifically detects a band for M13 Bacteriophage Coat Protein g8p (UniProt: P69541) at a molecular weight of 5kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The M13 bacteriophage coat protein g8p also known simply as g8p plays a mechanical role in forming the protective sheath around the phage's single-stranded DNA. The molecular weight of g8p is approximately 5 kDa. It is an important component of the M13 bacteriophage typically expressed within the bacterial host after phage infection. As one of the coat proteins g8p drives the assembly of new phage particles by arranging itself into helical structures that encapsulate the viral genome.

Biological function summary

G8p's function extends beyond just structural support for the phage. It participates in the assembly of the M13 phage which is part of the extrinsic complex necessary for phage propagation. During this process g8p cooperates closely with other proteins to manage the packaging of genetic material and ensure successful phage extrusion from the bacterial host. Functionally g8p aids in maintaining phage integrity and viability directly affecting the efficiency of phage propagation within the microbial environment.

Pathways

G8p has an essential role in the M13 bacteriophage lifecycle. It facilitates the phage's extrusion pathway where newly formed phage particles exit the bacterial cell. This pathway involves interactions with proteins like the M13 p9 (g9p) protein ensuring efficient phage release and infection of nearby bacterial cells. The interaction among these proteins through the extrusion pathway is critical for the replication and spread of the M13 bacteriophage.

G8p as a component of phage display technology interacts indirectly with a variety of disorders particularly bacterial infections. M13 phage display systems use g8p to present peptides or proteins on the phage surface aiding in identifying molecules that can target infectious agents. Although g8p's primary function does not link directly to specific diseases its application in phage display connects it with therapeutic and diagnostic developments potentially interacting with proteins involved in bacterial pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid is made of gently-curved alpha-helix rods and displays a filamentous structure with a length of 760-1950 nm and a width of 6 nm (By similarity). Belongs to the capsids of class I filamentous phages, which have a helical rise of about 16 Angstrom and a helical twist of about 36 degrees (PubMed : 25587134). The virion assembly and budding take place at the host inner membrane (Probable).

See full target information Capsid protein G8P

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eads9581 PubMed40117376

2025

Selective promotion of sensory innervation-mediated immunoregulation for tissue repair.

Applications

Unspecified application

Species

Unspecified reactive species

Kaicheng Xu,Kaile Wu,Liang Chen,Yubin Zhao,Hengyuan Li,Nong Lin,Zhaoming Ye,Jianbin Xu,Donghua Huang,Xin Huang

Microbiology spectrum 11:e0144623 PubMed37811937

2023

M13KO7 bacteriophage enables Potato Virus Y detection.

Applications

Unspecified application

Species

Unspecified reactive species

Haneul Seo,Sang-Ho Cho,Thuy T B Vo,Ahlim Lee,Sungrae Cho,Sol Kang,Eui-Joon Kil,Hee-Seong Byun,Mi-Gi Lee,Myung-Hee Kwon,Woo-Jae Chung,Young-Gyu Lee,Sukchan Lee

mAbs 15:2177978 PubMed36803166

2023

SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah-Jane Kellmann,Christian Hentrich,Mateusz Putyrski,Hanh Hanuschka,Manuel Cavada,Achim Knappik,Francisco Ylera

Cancer gene therapy : PubMed31902944

2020

Identification of folate receptor α (FRα) binding oligopeptides and their evaluation for targeted virotherapy applications.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah L Hulin-Curtis,James A Davies,Davor Nestić,Emily A Bates,Alexander T Baker,Tabitha G Cunliffe,Dragomira Majhen,John D Chester,Alan L Parker

Human antibodies and hybridomas 6:113-8 PubMed8597626

1995

Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

E V Meulemans,L J Nieland,W H Debie,F C Ramaekers,G J van Eys

Journal of molecular biology 244:353-60 PubMed7527464

1994

Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

E V Meulemans,R Slobbe,P Wasterval,F C Ramaekers,G J van Eys

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise