Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody that is used to detect M13 Bacteriophage Coat Protein g8p in Western Blot, Flow Cytometry. Suitable for Enterobacteria phage M13 samples.
- Trusted since 2002
Images
![Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225), expandable thumbnail](https://content.abcam.com/products/images/m13-bacteriophage-coat-protein-g8p-antibody-rl-ph2-ab9225--western-blot-img66717.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225)")
Publications
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- mAbs 15:21779782023SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah-Jane Kellmann,Christian Hentrich,Mateusz Putyrski,Hanh Hanuschka,Manuel Cavada,Achim Knappik,Francisco YleraPubMed 36803166
- Cancer gene therapy :2020Identification of folate receptor α (FRα) binding oligopeptides and their evaluation for targeted virotherapy applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah L Hulin-Curtis et. al.PubMed 31902944
- Human antibodies and hybridomas 6:113-81995Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE V Meulemans,L J Nieland,W H Debie,F C Ramaekers,G J van EysPubMed 8597626
- Journal of molecular biology 244:353-601994Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE V Meulemans,R Slobbe,P Wasterval,F C Ramaekers,G J van EysPubMed 7527464
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Enterobacteria phage M13 Capsid protein G8P. Database link P69541
Reactivity data
Select an application| WB | Flow Cyt | ICC | |
|---|---|---|---|
| Enterobacteria phage M13 | Predicted | Expected | Expected |
| Recombinant full length protein - Enterobacteria phage M13 | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Enterobacteria phage M13 | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Enterobacteria phage M13 | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Enterobacteria phage M13 | Dilution info 1/25 - 1/200 | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Enterobacteria phage M13 | Dilution info - | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Enterobacteria phage M13 | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Enterobacteria phage M13 | Dilution info - | Notes - |
Target data
Function
Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane.
Additional Targets
M13 Bacteriophage Coat Protein g8p
Alternative names
Capsid protein G8P, Coat protein B, Gene 8 protein, M13 procoat, Major coat protein, G8P, VIII
Recommended products
Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody that is used to detect M13 Bacteriophage Coat Protein g8p in Western Blot, Flow Cytometry. Suitable for Enterobacteria phage M13 samples.
- Trusted since 2002
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Enterobacteria phage M13 Capsid protein G8P. Database link P69541
- Clone number
- RL-ph2
- Purification technique
- Affinity purification Protein G
- Specificity
RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The M13 bacteriophage coat protein g8p also known simply as g8p plays a mechanical role in forming the protective sheath around the phage's single-stranded DNA. The molecular weight of g8p is approximately 5 kDa. It is an important component of the M13 bacteriophage typically expressed within the bacterial host after phage infection. As one of the coat proteins g8p drives the assembly of new phage particles by arranging itself into helical structures that encapsulate the viral genome.
- Biological function summary
G8p's function extends beyond just structural support for the phage. It participates in the assembly of the M13 phage which is part of the extrinsic complex necessary for phage propagation. During this process g8p cooperates closely with other proteins to manage the packaging of genetic material and ensure successful phage extrusion from the bacterial host. Functionally g8p aids in maintaining phage integrity and viability directly affecting the efficiency of phage propagation within the microbial environment.
- Pathways
G8p has an essential role in the M13 bacteriophage lifecycle. It facilitates the phage's extrusion pathway where newly formed phage particles exit the bacterial cell. This pathway involves interactions with proteins like the M13 p9 (g9p) protein ensuring efficient phage release and infection of nearby bacterial cells. The interaction among these proteins through the extrusion pathway is critical for the replication and spread of the M13 bacteriophage.
- Associated diseases and disorders
G8p as a component of phage display technology interacts indirectly with a variety of disorders particularly bacterial infections. M13 phage display systems use g8p to present peptides or proteins on the phage surface aiding in identifying molecules that can target infectious agents. Although g8p's primary function does not link directly to specific diseases its application in phage display connects it with therapeutic and diagnostic developments potentially interacting with proteins involved in bacterial pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225), expandable thumbnail](https://content.abcam.com/products/images/m13-bacteriophage-coat-protein-g8p-antibody-rl-ph2-ab9225--western-blot-img66717.jpg "Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)")
Image courtesy of an anonymous customer review.Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)
Primary antibody incubated for 16 hours at 4°C.
Blocking step performed using 5% milk for 1 hour at room temperature.All lanes: Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution
All lanes: whole cell lysate prepared from XL10Gold cells at 50 µg
SecondaryAll lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab6728) at 1/2000 dilution
Predicted band size: 8 kDa
Observed band size: 6 kDa
Downloads
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