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Rabbit Recombinant Monoclonal M6PR (cation dependent) antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 10 publications.


Images

Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153), expandable thumbnail
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153), expandable thumbnail
  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Rat
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/80
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

Alternative names

MPR46, MPRD, M6PR, Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46

Recommended products

Rabbit Recombinant Monoclonal M6PR (cation dependent) antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7691
Purification technique
Affinity purification Protein A
Dissociation constant
6.3 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

M6PR or mannose-6-phosphate receptor (cation dependent) is a protein involved in the transport of lysosomal enzymes from the Golgi apparatus to the lysosome. Alternate names for M6PR include CD-MPR and MPR46 reflecting its function and molecular weight of approximately 46 kDa. M6PR is expressed in various tissues but primarily found in the Golgi apparatus where it engages with lysosomal enzymes. This receptor is an important element in the delivery system that ensures lysosomal enzymes reach their intended cellular destination.

Biological function summary

M6PR acts as a sorting receptor by binding mannose-6-phosphate-tagged enzymes in the Golgi. It is involved in a complex trafficking mechanism that involves clathrin-coated vesicles. The receptor recognizes and binds to its ligands in the Golgi directing them to endosomes and eventually fusing with lysosomes. M6PR plays a critical role in maintaining lysosome functionality by regulating enzyme availability which is essential for cellular waste degradation and resource recycling.

Pathways

The protein M6PR participates in the lysosomal enzyme targeting pathway that ensures proper enzyme sorting and delivery within cells. This pathway overlaps with the Golgi-to-endosome transport vesicle biogenesis involving proteins like clathrin and adaptin. M6PR interacts with proteins in the sorting pathway such as GGA adaptors and Hsc70 which assist in the recognition and transport processes necessary for lysosome integrity and function.

Associated diseases and disorders

The malfunction of M6PR links to disorders like I-cell disease and mucolipidosis II characterized by defective lysosomal enzyme targeting. Impaired M6PR function can lead to the accumulation of undigested substrates within the lysosome causing cellular dysfunction. The receptor's failure can also indirectly affect protein interactions in diseases like mucolipidosis where relationships with proteins such as IGF2R can get altered further influencing cellular pathways and leading to various pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153), expandable thumbnail

    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: Empty
    Lane 3: M6PR knockout HAP1 whole cell lysate (20 μg)
    Lane 4: A549 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Predicted band size: 31 kDa

    Observed band size: 46 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153), expandable thumbnail

    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 0.8 µg/mL

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: Mouse kidney lysates at 15 µg

    Lane 3: Rat kidney lysates at 15 µg

    Lane 4: Rat spleen lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 31 kDa

  • Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153), expandable thumbnail

    Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    All lanes: Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153) at 1/1000 dilution

    Lane 1: A549 lysates at 10 µg

    Lane 2: Human uterus lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa

  • OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153), expandable thumbnail

    OI-RD Scanning - Anti-M6PR (cation dependent) antibody [EPR7691] (ab134153)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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