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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal M6PR (cation dependent) antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 10 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Not recommended | Tested | Tested |
Mouse | Tested | Not recommended | Expected | Expected |
Rat | Tested | Not recommended | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/80 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46, MPRD, MPR46, M6PR
Rabbit Recombinant Monoclonal M6PR (cation dependent) antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 10 publications.
Cation-dependent mannose-6-phosphate receptor, CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46, MPRD, MPR46, M6PR
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR7691
Affinity purification Protein A
6.3 x 10-11 M
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
M6PR acts as a sorting receptor by binding mannose-6-phosphate-tagged enzymes in the Golgi. It is involved in a complex trafficking mechanism that involves clathrin-coated vesicles. The receptor recognizes and binds to its ligands in the Golgi directing them to endosomes and eventually fusing with lysosomes. M6PR plays a critical role in maintaining lysosome functionality by regulating enzyme availability which is essential for cellular waste degradation and resource recycling.
M6PR or mannose-6-phosphate receptor (cation dependent) is a protein involved in the transport of lysosomal enzymes from the Golgi apparatus to the lysosome. Alternate names for M6PR include CD-MPR and MPR46 reflecting its function and molecular weight of approximately 46 kDa. M6PR is expressed in various tissues but primarily found in the Golgi apparatus where it engages with lysosomal enzymes. This receptor is an important element in the delivery system that ensures lysosomal enzymes reach their intended cellular destination.
The protein M6PR participates in the lysosomal enzyme targeting pathway that ensures proper enzyme sorting and delivery within cells. This pathway overlaps with the Golgi-to-endosome transport vesicle biogenesis involving proteins like clathrin and adaptin. M6PR interacts with proteins in the sorting pathway such as GGA adaptors and Hsc70 which assist in the recognition and transport processes necessary for lysosome integrity and function.
The malfunction of M6PR links to disorders like I-cell disease and mucolipidosis II characterized by defective lysosomal enzyme targeting. Impaired M6PR function can lead to the accumulation of undigested substrates within the lysosome causing cellular dysfunction. The receptor's failure can also indirectly affect protein interactions in diseases like mucolipidosis where relationships with proteins such as IGF2R can get altered further influencing cellular pathways and leading to various pathological conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: Empty
Lane 3: M6PR knockout HAP1 whole cell lysate (20 μg)
Lane 4: A549 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - unpurified ab134153 observed at 46 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab134153 was shown to specifically react with M6PR when M6PR knockout samples were used. Wild-type and M6PR knockout samples were subjected to SDS-PAGE. ab134153 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153)
Predicted band size: 31 kDa
Observed band size: 46 kDa
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling M6PR (cation dependent) with Purified ab134153 at 1:100 dilution (8.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153) at 0.8 µg/mL
Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: Mouse kidney lysates at 15 µg
Lane 3: Rat kidney lysates at 15 µg
Lane 4: Rat spleen lysates at 15 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 31 kDa
Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling M6PR (cation dependent) with purified ab134153 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
All lanes: Western blot - Anti-M6PR (cation dependent) antibody [EPR7691] (AB134153) at 1/1000 dilution
Lane 1: A549 lysates at 10 µg
Lane 2: Human uterus lysates at 10 µg
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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