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Mouse Monoclonal M6PR (cation dependent) antibody. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 127 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (AB2733), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (AB2733), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (AB2733), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (AB2733), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (AB2733), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IF
Human
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
African green monkey
Not recommended
Not recommended
Not recommended
Cow
Not recommended
Not recommended
Not recommended
Hamster
Not recommended
Not recommended
Not recommended
Primates
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Hamster

Dilution info

-

Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Primates

Dilution info

-

Notes

-

Species

African green monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Hamster

Dilution info

-

Notes

Abcam recommends using BSA as the blocking agent.

Species

Human

Dilution info

-

Notes

Abcam recommends using BSA as the blocking agent.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Primates

Dilution info

-

Notes

-

Species

African green monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-10.00000 µg/mL

Notes

-

Not recommended
Not recommended

Species

Hamster, Mouse, Rat, Cow, Primates, African green monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

Alternative names

Recommended products

Mouse Monoclonal M6PR (cation dependent) antibody. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 127 publications.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

2G11

Purification technique

Affinity purification Protein G

Epitope

This antibody is shown to recognize an epitope in the extracellular domain of Mannose 6 Phosphate Receptor.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.

Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

Associated diseases and disorders

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

    ab2733 staining IGF2R in wild-type HAP1 cells (top panel) and IGF2R knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2733 at 1ug/ml and Anti-beta Tubulin antibody - Loading Control ab6046 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

    ICC/IF image of ab2733 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2733, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733), expandable thumbnail
    Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

    Immunofluorescent imaging of human cells (U2OS) with ab2733 confirms the specificity of this antibody, with the expected perinuclear vesicular staining of lysosomes.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

    ab2733 positively staining formaldehyde fixed Human HEK 293 cells (red) in conjunction with goat anti mouse (Alexa 546). Nuclear staining was obtained using Hoechst.
    This image is an edited version of an image received courtesy of an Abreview submitted by Kun Liu on 19 September 2005. We do not have any further information relating to this image.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

    ICC/IF image of ab2733 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2733, 10μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

  • Flow Cytometry (Intracellular) - Anti-M6PR (cation independent) antibody [2G11] (ab2733), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

    Overlay histogram showing HeLa cells stained with ab2733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2733, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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