AB12894

Anti-M6PR (cation independent) antibody

(6 Reviews)

(5 Publications )

Rabbit Polyclonal M6PR (cation independent) antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (AB12894)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (AB12894)

HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4μg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.

This image was submitted as part of a review by Kirk McManus, University of British Columbia

ICC/IF

AbReview6913****

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (AB12894)

ab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "4 µg/mL", "ICCIF-species-notes": "<p>This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.</p>" }, "African green monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.

Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed : 18817523, PubMed : 2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed : 18817523, PubMed : 2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed : 18817523). This receptor also binds IGF2 (PubMed : 18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed : 10900005).

See full target information IGF2R

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:11341-11357 PubMed29858247

2018

Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-β clearance in human astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren K Fong,Max M Yang,Rodrigo Dos Santos Chaves,Sol M Reyna,Vanessa F Langness,Grace Woodruff,Elizabeth A Roberts,Jessica E Young,Lawrence S B Goldstein

Gene therapy 20:308-17 PubMed22622241

2012

Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells.

Applications

Unspecified application

Species

Human

Y Liu,K-I Joo,P Wang

Human molecular genetics 20:2322-32 PubMed21487022

2011

Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability.

Applications

ICC/IF

Species

Human

Yiguo Shen,Hsien-Yang Lee,Joel Rawson,Sunil Ojha,Patricia Babbitt,Ying-Hui Fu,Louis J Ptácek

The Journal of comparative neurology 517:296-312 PubMed19757494

2009

Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan H Clarke,Piers C Emson,Robin F Irvine

The American journal of pathology 174:957-69 PubMed19164507

2009

The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells.

Applications

ICC/IF

Species

African green monkey

Kazuchika Nishitsuji,Takami Tomiyama,Kenichi Ishibashi,Kazuhiro Ito,Rie Teraoka,Mary P Lambert,William L Klein,Hiroshi Mori

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-M6PR (cation independent) antibody

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (AB12894)

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (AB12894)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise