Name: Anti-M6PR (cation independent) antibody [EPR6599]
SKU: ab124767
Rating: 5 (7 reviews)

Anti-M6PR (cation independent) antibody EPR6599 is a rabbit monoclonal antibody that is used in M6PR (cation independent) Western blot, IHC, ICC/IF, Flow cytometry. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with IGF2R knockout cell line validation
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR6599 is cited in over 80 publications

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Alexa Fluor® 555 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker
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ab184807
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Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker
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PE Anti-M6PR (cation independent) antibody [EPR6599]
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Anti-M6PR (cation independent) antibody [EPR6599] - BSA and Azide free
ab203437
Conjugated
Alexa Fluor® 594 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker
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APC Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker

Images

Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (AB124767), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Expected
Rat	Tested	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/500	Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250 - 1/500	Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/250 - 1/500	Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50000 - 1/200000	Notes -
Species Human	Dilution info 1/50000 - 1/200000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/500	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

13 products for Alternative Product

Target data

See full target information IGF2R

Function

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed:18817523, PubMed:2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed:18817523, PubMed:2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed:18817523). This receptor also binds IGF2 (PubMed:18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed:10900005).

Alternative names

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR6599
Purification technique: Affinity purification Protein A
Dissociation constant: 3.9 x 10^-12 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Product Specifications
Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) specifically detects M6PR (cation independent) (UniProt ID: P11717; Molecular weight: 270kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) has been confirmed by testing in knockout samples.
Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) has been cited over 72 times in peer reviewed journals and is trusted by the scientific community.
Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) has 7 independent reviews from customers.

Related Products
Antibody clone EPR6599 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 405, Alexa Fluor® 594, Alexa Fluor® 555, APC, PE (ab184807, ab198324, ab198515, ab203437, ab203438, ab225131, ab225132).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: M6PR (cation independent) knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: A549 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124767 observed at 274 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab124767 was shown to specifically react with M6PR (cation independent) in wild-type HAP1 cells as signal was lost in M6PR (cation independent) knockout cells. Wild-type and M6PR (cation independent) knockout samples were subjected to SDS-PAGE. ab124767 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Lane 1: Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Lanes 2 - 4: Western blot - Anti-M6PR (cation independent) antibody [EPR6599] - BSA and Azide free (Anti-M6PR (cation independent) antibody [EPR6599] - BSA and Azide free ab226090) at 1/50000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: M6PR (cation independent) knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: A549 whole cell lysate at 20 µg
Predicted band size: 274 kDa
Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
ab124767 staining M6PR in wild-type HAP1 cells (top panel) and IGF2R knockout HAP1 cells (bottom panel). The cells were fixed with 100% MeOH for 5 min. , permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124767 at 1μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Blocking and diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) at 1/50000 dilution
Lane 1: C6 (rat glioma) whole cell lysate at 20 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg
Lane 3: NIH/3T3 (mouse embryo) whole cell lysate at 20 µg
Lane 4: Mouse heart tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 100 kDa, 107 kDa, 183 kDa, 274 kDa, 35 kDa
Observed band size: 120 kDa, 300 kDa, 43 kDa
Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
All lanes: Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) at 1/50000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: Caco-2 cell lysate at 10 µg
Secondary
All lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Blocking and diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) at 1/200000 dilution
Lane 1: Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg
Lane 2: HEK293 (human embryonic kidney) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 274 kDa
Observed band size: 300 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunohistochemical staining of paraffin embedded rat colon tissue section labelling M6PR with purified ab124767 at dilution of 1/500. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunohistochemical staining of paraffin embedded mouse colon tissue section labelling M6PR with purified ab124767 at dilution of 1/500. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunohistochemical staining of paraffin embedded human thyroid carcinoma tissue section labelling M6PR with purified ab124767 at dilution of 1/500. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human papillary carcinoma tissue section labelling Mannose 6 Phosphate Receptor (Cation independent) with unpurified ab124767 at dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue section labelling Mannose 6 Phosphate Receptor (Cation independent) with unpurified ab124767 at dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling M6PR with purified ab124767 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000) using Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei couterstained with DAPI (blue).
For negative control 1, rabbit primary antibody was used, followed by anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120). For negative control 2, mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) was used followed by anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077).
Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Immunocytochemistry/immunofluorescence analysis of 293T cells labelling Mannose 6 Phosphate Receptor (Cation independent) with unpurified ab124767 at dilution of 1/100.
Flow Cytometry (Intracellular) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling M6PR with purified ab124767 at 1/150 (red). Cells were fixed with 4% paraformaldehyde. Alexa Fluorr^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
OI-RD Scanning - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.