AB32815

Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker

Name: Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (ab32815) | Abcam
Brand: Abcam Limited
SKU: AB32815
Price: 565 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(31 Publications)

Rabbit Polyclonal M6PR (cation independent) antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 31 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow IGF2R.

View Alternative Names

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

ab32815 staining human normal left ventricle of heart. Staining is localized to lysosome and lysosomal membrane.
Left panel : with primary antibody duluted at 1 : 1000. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplifi

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunofluorescent analysis of Mannose 6 Phosphate Receptor (Cation independent)(green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Mannose 6 Phosphate Receptor (Cation independent) antibody (ab32815) in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

ICC/IF image of ab32815 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32815, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunofluorescent analysis of Mannose 6 Phosphate Receptor (Cation independent) (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Mannose 6 Phosphate Receptor (Cation independent) antibody (ab32815) in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunofluorescent analysis of Mannose 6 Phosphate Receptor (Cation independent) (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Mannose 6 Phosphate Receptor (Cation independent) antibody (ab32815) in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Cow IGF2R.

P08169

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.

Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed : 18817523, PubMed : 2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed : 18817523, PubMed : 2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed : 18817523). This receptor also binds IGF2 (PubMed : 18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed : 10900005).

See full target information IGF2R

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 22:154 PubMed38987765

2024

Endosomal protein DENND10/FAM45A integrates extracellular vesicle release with cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shenqing Sun,Qian Li,Ganggang Liu,Xiaoheng Huang,Aiqing Li,Haoran Guo,Lijuan Qi,Jie Zhang,Jianrui Song,Xiong Su,Yanling Zhang

Nature communications 14:3911 PubMed37400440

2023

Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Calcagni',Leopoldo Staiano,Nicolina Zampelli,Nadia Minopoli,Niculin J Herz,Giuseppe Di Tullio,Tuong Huynh,Jlenia Monfregola,Alessandra Esposito,Carmine Cirillo,Aleksandar Bajic,Mahla Zahabiyon,Rachel Curnock,Elena Polishchuk,Luke Parkitny,Diego Luis Medina,Nunzia Pastore,Peter J Cullen,Giancarlo Parenti,Maria Antonietta De Matteis,Paolo Grumati,Andrea Ballabio

Cells 11: PubMed35159308

2022

Autophagy and Lysosomal Functionality in CMT2B Fibroblasts Carrying the RAB7 Mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Romano,Victoria Stefania Del Fiore,Paola Saveri,Ilaria Elena Palamà,Chiara Pisciotta,Davide Pareyson,Cecilia Bucci,Flora Guerra

Journal of virology 95: PubMed33115879

2020

Precise Triggering and Chemical Control of Single-Virus Fusion within Endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav Haldar,Kenta Okamoto,Rebecca A Dunning,Peter M Kasson

Journal of cell science 133: PubMed32907852

2020

Invariant chain regulates endosomal fusion and maturation through an interaction with the SNARE Vti1b.

Applications

Unspecified application

Species

Unspecified reactive species

Azzurra Margiotta,Dominik M Frei,Ingrid Hegnes Sendstad,Lennert Janssen,Jacques Neefjes,Oddmund Bakke

Cellular and molecular life sciences : CMLS 78:351-372 PubMed32280996

2020

Alteration of the late endocytic pathway in Charcot-Marie-Tooth type 2B disease.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Romano,Cristina Rivellini,Maria De Luca,Rossana Tonlorenzi,Raffaella Beli,Fiore Manganelli,Maria Nolano,Lucio Santoro,Eeva-Liisa Eskelinen,Stefano C Previtali,Cecilia Bucci

Journal of neurochemistry 156:290-308 PubMed32072649

2020

Gene therapy to the blood-brain barrier with resulting protein secretion as a strategy for treatment of Niemann Picks type C2 disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Hede,Christine B Christiansen,Christian W Heegaard,Torben Moos,Annette Burkhart

Molecular and cellular biology 39: PubMed31427458

2019

PIKfyve Deficiency in Myeloid Cells Impairs Lysosomal Homeostasis in Macrophages and Promotes Systemic Inflammation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Hee Min,Aae Suzuki,Lehn Weaver,Jessica Guzman,Yutein Chung,Huiyan Jin,Francina Gonzalez,Claire Trasorras,Liang Zhao,Lynn A Spruce,Steven H Seeholzer,Edward M Behrens,Charles S Abrams

Cell death and differentiation 27:310-328 PubMed31142807

2019

The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Finetti,Chiara Cassioli,Valentina Cianfanelli,Anna Onnis,Eugenio Paccagnini,Anna Kabanova,Cosima T Baldari

Experimental cell research 380:55-68 PubMed30981667

2019

A role for Rab11 in the homeostasis of the endosome-lysosomal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Khalisah L Zulkefli,Fiona J Houghton,Prajakta Gosavi,Paul A Gleeson

View all publications

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Product promise

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Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (AB32815)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

Product promise