AB242199

Anti-MACC1 antibody [CL0856]

Name: Anti-MACC1 antibody [CL0856] (ab242199) | Abcam
Brand: Abcam Limited
SKU: AB242199

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(3 Publications)

Mouse Monoclonal MACC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MACC1 aa 1-100.

View Alternative Names

Metastasis-associated in colon cancer protein 1, SH3 domain-containing protein 7a5, MACC1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human uterus tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MACC1 antibody [CL0856] (AB242199)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MACC1 antibody [CL0856] (AB242199)

Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling MACC1 using ab242199 at 10 μg/ml (green). Nuclear probe is visualized in blue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human colon tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human stomach tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human rectum tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MACC1 with ab242199 at a 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0856

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MACC1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q6ZN28

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MACC1 known as Metastasis-Associated in Colon Cancer 1 plays an important mechanical role in the regulation of cell proliferation and mobility. It has a molecular mass of approximately 82 kDa. This protein expresses prominently in the colon and other epithelial tissues where it assists processes linked to cancer progression. Researchers acknowledge its involvement in cellular signaling making it a focus of biomedical studies.

Biological function summary

MACC1 affects tumor growth and metastasis through its role as a regulator of the HGF/MET signaling pathway. It influences gene expression that promotes invasive behavior in cancer cells. MACC1 does not form a larger complex with other proteins in its function but coordinates with several signaling molecules to control gene transcription linked to metastasis. This ability highlights its significance in oncological contexts.

Pathways

MACC1 protein heavily interacts with the HGF/MET pathway which is central to cell growth movement and survival. MACC1 boosts MET expression and pathway activation thereby amplifying signals for cell invasion. It also ties to the Wnt signaling pathway and influences processes related to cell adhesion and metastasis. Through these pathways MACC1 contributes to signaling cascades that enhance cellular capabilities critical to tumor development.

MACC1 is closely tied to colorectal cancer where it predicts aggressive tumor behavior and patient prognosis. Gastric cancer also witnesses MACC1's influence where it serves similar roles in tumor progression. Through its interaction with the MET protein MACC1 exacerbates disease states by promoting invasion and resistance. This connection between MACC1 and specific tumors renders it a potential target for therapeutic interventions specific to these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.

See full target information MACC1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:283 PubMed40462244

2025

ECM remodeling by PDGFRβ+ dental pulp stem cells drives angiogenesis and pulp regeneration via integrin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tiankai Di,Lulu Wang,Chao Feng,Jinlong Xu,Bo Shao,Baixiang Cheng,Liying Wang,Xiao Zhang,Ling Zhang,Yujiang Chen

Oncology letters 23:84 PubMed35126726

2022

Alpha-fetoprotein can promote gastric cancer progression via upregulation of metastasis-associated colon cancer 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Mao,Jun Wang,Fen Luo

Oncology research 28:913-927 PubMed34493358

2021

Long Noncoding RNA LINC01703 Exacerbates the Malignant Properties of Non-Small Cell Lung Cancer by Upregulating MACC1 in a MicroRNA-605-3p-Mediated Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Wang,Xinyu Zhang,Xuedong Zhang,Xuedong Jiang,Wenya Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MACC1 antibody [CL0856]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Immunocytochemistry/ Immunofluorescence - Anti-MACC1 antibody [CL0856] (AB242199)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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