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Mouse Monoclonal MACC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MACC1 aa 1-100.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MACC1 antibody [CL0856] (AB242199), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (AB242199), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human MACC1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q6ZN28

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Orangutan
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/1000.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Orangutan
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2.00000-10.00000 µg/mL
Notes

Fixation/Permeabilization: PFA/Triton X-100

Predicted
Predicted

Species
Orangutan
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.

Alternative names

Metastasis-associated in colon cancer protein 1, SH3 domain-containing protein 7a5, MACC1

Recommended products

Mouse Monoclonal MACC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MACC1 aa 1-100.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human MACC1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q6ZN28
Clone number
CL0856
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MACC1 known as Metastasis-Associated in Colon Cancer 1 plays an important mechanical role in the regulation of cell proliferation and mobility. It has a molecular mass of approximately 82 kDa. This protein expresses prominently in the colon and other epithelial tissues where it assists processes linked to cancer progression. Researchers acknowledge its involvement in cellular signaling making it a focus of biomedical studies.

Biological function summary

MACC1 affects tumor growth and metastasis through its role as a regulator of the HGF/MET signaling pathway. It influences gene expression that promotes invasive behavior in cancer cells. MACC1 does not form a larger complex with other proteins in its function but coordinates with several signaling molecules to control gene transcription linked to metastasis. This ability highlights its significance in oncological contexts.

Pathways

MACC1 protein heavily interacts with the HGF/MET pathway which is central to cell growth movement and survival. MACC1 boosts MET expression and pathway activation thereby amplifying signals for cell invasion. It also ties to the Wnt signaling pathway and influences processes related to cell adhesion and metastasis. Through these pathways MACC1 contributes to signaling cascades that enhance cellular capabilities critical to tumor development.

Associated diseases and disorders

MACC1 is closely tied to colorectal cancer where it predicts aggressive tumor behavior and patient prognosis. Gastric cancer also witnesses MACC1's influence where it serves similar roles in tumor progression. Through its interaction with the MET protein MACC1 exacerbates disease states by promoting invasion and resistance. This connection between MACC1 and specific tumors renders it a potential target for therapeutic interventions specific to these cancers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MACC1 antibody [CL0856] (ab242199)

    Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling MACC1 using ab242199 at 10 μg/ml (green). Nuclear probe is visualized in blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human rectum tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human colon tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for MACC1 with ab242199 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACC1 antibody [CL0856] (ab242199)

    Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MACC1 with ab242199 at a 1/500 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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