AB117418

Anti-MACF1 antibody

Name: Anti-MACF1 antibody (ab117418) | Abcam
Brand: Abcam Limited
SKU: AB117418
Price: 525 USD
Availability: InStock

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(10 Publications )

Rabbit Polyclonal MACF1 antibody. Suitable for WB, IP, ICC/IF, IHC-P, ELISA and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Macf1 aa 3650 to C-terminus.

View Alternative Names

Acf7, Aclp7, Kiaa0754, Macf, Macf1, Actin cross-linking family 7

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACF1 antibody (AB117418)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACF1 antibody (AB117418)

ab117418 at 1/250 dilution staining MACF1 in paraformaldehyde fixed Mouse kidney tissue by Immunofluorescence. Antigen retrival was performed in a pressure cooker for 15 minutes.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ELISA, IHC-P, ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Mouse Macf1 aa 3650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/200", "IP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MACF1 also known as Microtubule Actin Cross-linking Factor 1 or ACF7 is a large protein with a mass of approximately 615 kDa. This protein serves as a cytoskeletal crosslinker playing an essential role in linking microtubules and actin filaments. MACF1 localizes to the cytoplasm and is expressed in various tissues throughout the body including muscle and brain. Its ability to interact with both microtubules and actin is central to maintaining cellular structure and dynamics.

Biological function summary

MACF1 facilitates cellular processes related to mechanical stability and intracellular transport. It integrates into diverse macromolecular assemblies including complexes that regulate cell polarization and migration. By influencing the architecture and dynamics of the cytoskeleton MACF1 contributes to the mechanical resilience and motility of cells. This protein also interacts with other cytoskeletal regulators supporting its role in dynamic cellular environments.

Pathways

There are several that MACF1 is actively involved with that intricately control cytoskeletal dynamics. It plays a major part in the Wnt signaling pathway interacting with proteins like GSK-3β to influence cell fate and development. MACF1 also crosslinks with the Hippo signaling pathway important for the regulation of organ size and cell proliferation. These pathways show how MACF1 intersects with key regulatory axes that coordinate cell movement and stability.

Mutations or dysfunctions in MACF1 often link to neurological disorders and muscular dystrophies. In Parkinson's disease disruptions in MACF1-associated signaling pathways might affect neuronal stability and survival. In muscular dystrophy defective association between MACF1 and dystrophin complicates muscle cell integrity. The impaired interactions between MACF1 and these associated proteins can profoundly impact tissue function and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform 2. F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules (PubMed : 16815997, PubMed : 18854161, PubMed : 21295697). Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (By similarity). Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane (PubMed : 16815997). Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics (PubMed : 18854161). Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration (By similarity). May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4 (By similarity). Plays a key role in wound healing and epidermal cell migration (PubMed : 21295697). Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells (PubMed : 21295697). As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development (PubMed : 26526844).

See full target information Macf1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7430 PubMed39198412

2024

hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Abdolhossein Zare,Saeede Salehi,Jakob Bader,Cornelius Schneider,Utz Fischer,Alexander Veh,Panagiota Arampatzi,Matthias Mann,Michael Briese,Michael Sendtner

Journal of cellular and molecular medicine : PubMed34133068

2021

MACF1 alleviates aging-related osteoporosis via HES1.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Yin,Ye Tian,Lifang Hu,Yang Yu,Zixiang Wu,Yan Zhang,Xue Wang,Zhiping Miao,Airong Qian

Theranostics 11:5585-5604 PubMed33859765

2021

Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Dijie Li,Jin Liu,Chaofei Yang,Ye Tian,Chong Yin,Lifang Hu,Zhihao Chen,Fan Zhao,Ru Zhang,Aiping Lu,Ge Zhang,Airong Qian

Cell death and differentiation 28:2160-2178 PubMed33664480

2021

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Hu,Chong Yin,Dong Chen,Zixiang Wu,Shujing Liang,Yu Zhang,Zizhan Huang,Shuyu Liu,Xia Xu,Zhihao Chen,Yi Zhang,Airong Qian

Cells 9: PubMed32143362

2020

Mesenchymal MACF1 Facilitates SMAD7 Nuclear Translocation to Drive Bone Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhao,Xiaoli Ma,Wuxia Qiu,Pai Wang,Ru Zhang,Zhihao Chen,Peihong Su,Yan Zhang,Dijie Li,Jianhua Ma,Chaofei Yang,Lei Chen,Chong Yin,Ye Tian,Lifang Hu,Yu Li,Ge Zhang,Xiaoyang Wu,Airong Qian

Molecular and cellular endocrinology 494:110494 PubMed31260729

2019

Microtubule actin crosslinking factor 1 (MACF1) knockdown inhibits RANKL-induced osteoclastogenesis via Akt/GSK3β/NFATc1 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lin,Yunyun Xiao,Zhihao Chen,Jianhua Ma,Wuxia Qiu,Kewen Zhang,Fang Xu,Kai Dang,Airong Qian

The Journal of cell biology 218:1686-1705 PubMed30842214

2019

MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Oury,Yun Liu,Ana Töpf,Slobodanka Todorovic,Esthelle Hoedt,Veeramani Preethish-Kumar,Thomas A Neubert,Weichun Lin,Hanns Lochmüller,Steven J Burden

Journal of cellular physiology 233:5405-5419 PubMed29219183

2018

Mechanical unloading reduces microtubule actin crosslinking factor 1 expression to inhibit β-catenin signaling and osteoblast proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Yin,Yan Zhang,Lifang Hu,Ye Tian,Zhihao Chen,Dijie Li,Fan Zhao,Peihong Su,Xiaoli Ma,Ge Zhang,Zhiping Miao,Liping Wang,Airong Qian,Cory J Xian

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 37:1183-1189 PubMed28951359

2017

[MACF1 knockdown in glioblastoma multiforme cells increases temozolomide-induced cytotoxicity].

Applications

Unspecified application

Species

Unspecified reactive species

Si-di Xie,Zi-Yang Chen,Hai Wang,Min-Yi He,Yun-Tao Lu,Bing-Xi Lei,He-Zhen Li,Ya-Wei Liu,Song-Tao Qi

Journal of immunology research 2016:4039837 PubMed27635404

2016

Prophylactic Chronic Zinc Administration Increases Neuroinflammation in a Hypoxia-Ischemia Model.

Applications

Unspecified application

Species

Unspecified reactive species

Constantino Tomas-Sanchez,Victor Manuel Blanco-Alvarez,Juan Antonio Gonzalez-Barrios,Daniel Martinez-Fong,Guadalupe Garcia-Robles,Guadalupe Soto-Rodriguez,Eduardo Brambila,Maricela Torres-Soto,Alejandro Gonzalez-Vazquez,Ana Karina Aguilar-Peralta,José-Luis Garate-Morales,Luis-Angel Aguilar-Carrasco,Daniel I Limón,Jorge Cebada,Bertha Alicia Leon-Chavez

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MACF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MACF1 antibody (AB117418)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise