Rabbit Recombinant Monoclonal MIP-1 alpha/CCL3 antibody. Carrier free. Suitable for sELISA and reacts with Monkey, Human samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
sELISA | |
---|---|
Human | Expected |
Monkey | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
G0S19-1, MIP1A, SCYA3, G0S19-1, MIP1A, CCL3, SCYA3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha
Rabbit Recombinant Monoclonal MIP-1 alpha/CCL3 antibody. Carrier free. Suitable for sELISA and reacts with Monkey, Human samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR19900-147
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab242793 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MIP1a. The recommended pair for sandwich ELISA is:
Capture: Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) ab242545, Human MIP1a Capture Antibody (unconjugated)
Detector: ab242793, Human MIP1a Detector Antibody (unconjugated)
The reference range value is 0.14 - 100 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This supplementary information is collated from multiple sources and compiled automatically.
MIP-1 alpha also known as CCL3 is a chemokine that plays a critical role in the immune system. With a molecular weight of around 8 kDa it is primarily expressed in activated T-cells macrophages and fibroblasts. MIP-1 alpha acts as a signaling protein that binds to receptors on target cells directing the movement of immune cells. Researchers commonly refer to it using various alternative names such as MIP-1a and CCL3-alpha rat and it is frequently measured using assays like MIP-1 alpha ELISA for experimental analyses.
Proteins including MIP-1 alpha participate in the recruitment and activation of diverse immune cells aiding the inflammatory response. Through interaction with receptors like CCR1 and CCR5 MIP-1 alpha mobilizes cells like natural killer cells and monocytes. This chemokine does not generally form part of a larger protein complex but interacts directly with membrane receptors to exert its function in immune cell chemotaxis.
MIP-1 alpha operates in the context of both the inflammatory response and the chemokine signaling pathways. Its involvement in these pathways helps mediate the trafficking of leukocytes. Notably MIP-1 alpha interacts with proteins such as CCR1 and CCR5 to transmit signals that instigate immune responses. These proteins are integral to its role in modulating immunity providing targets for research into controlling inflammatory processes.
MIP-1 alpha closely relates to conditions such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis its overexpression can lead to excessive inflammation and joint destruction. Meanwhile in HIV infection MIP-1 alpha interacts with CCR5 a co-receptor necessary for the virus entry into cells. This dual relationship elucidates its potential as a therapeutic target in managing both inflammatory diseases and viral entry mechanisms.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Representative standard curve from corresponding SimpleStep ELISA® Kit (Human MIP-1 alpha/CCL3 ELISA Kit ab214569).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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