JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB245101

Anti-Macrophage Inflammatory Protein 1 gamma antibody [EPR20958-271] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Macrophage Inflammatory Protein 1 gamma antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Mrp2, Scya10, Scya9, Ccl9, C-C motif chemokine 9, CCF18, Macrophage inflammatory protein 1-gamma, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9, MIP-1-gamma, MRP-2

1 Images
Sandwich ELISA - Anti-Macrophage Inflammatory Protein 1 gamma antibody [EPR20958-271] - BSA and Azide free (Detector) (AB245101)
  • sELISA

Unknown

Sandwich ELISA - Anti-Macrophage Inflammatory Protein 1 gamma antibody [EPR20958-271] - BSA and Azide free (Detector) (AB245101)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab240689).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20958-271

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB240689

Mouse CCL9 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

  • 0
  • 0
Assay Kits

AB240689

Mouse CCL9 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse CCL9 ELISA Kit (AB240689)

  • 0
  • 0
Primary Antibodies

AB33682

Anti-LYVE1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (AB33682)

  • 4
  • 15
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB245082

Anti-Interferon gamma antibody [EPR20701-39] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Interferon gamma antibody [EPR20701-39] - BSA and Azide free (Detector) (AB245082)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab245101 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse CCL9. The recommended pair for sandwich ELISA is:
Capture: ab244828, Mouse CCL9 Capture Antibody (unconjugated)
Detector: ab245101, Mouse CCL9 Detector Antibody (unconjugated)
The reference range value is 2.2 - 140 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage Inflammatory Protein 1 gamma (MIP-1γ) also known as CCL9 functions as a chemokine involved in immune cell signaling. It weighs approximately 11 kDa. MIP-1γ attracts various immune cells to sites of inflammation within the body. The protein is expressed mainly in macrophages but you can also find it in other immune cells like dendritic cells. It plays a significant role in the body's response to inflammatory stimuli by mediating the recruitment of leukocytes.
Biological function summary

MIP-1γ contributes to immune response modulation by acting as a signaling molecule. It helps coordinate the movement and activation of immune cells such as monocytes and lymphocytes thereby modulating inflammation. Unlike some other chemokines MIP-1γ does not usually form a complex with proteins. Its primary role involves direct interactions with specific receptors on target cells that initiate intracellular signaling pathways leading to cell migration and activation.

Pathways

MIP-1γ engages in chemokine signaling and inflammatory response pathways. It intricately associates with the chemokine receptor CCR1 which is vital for mediating signal transduction processes essential for cell movement and immune surveillance. MIP-1γ also plays a role in the NF-kB signaling pathway important for transcription regulation and inflammatory response. This relationship highlights its involvement in orchestrating immune cell migration and the regulation of immune responses.

MIP-1γ connects to various conditions including chronic inflammatory diseases and certain cancers. For example it has a link to rheumatoid arthritis where it contributes to the persistence of inflammation by recruiting immune cells to affected joints. It is also associated with some types of leukemia where its expression levels can influence disease progression. Proteins like interleukin-1β interact with MIP-1γ in these conditions as they both participate in inflammatory responses that exacerbate disease states.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
See full target information Ccl9
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com