AB106151

Anti-Macrophage Inflammatory Protein 3 alpha antibody

Name: Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab106151) | Abcam
Brand: Abcam Limited
SKU: AB106151
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Macrophage Inflammatory Protein 3 alpha antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Ccl20.

View Alternative Names

Larc, Scya20, Ccl20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha

1 Images

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Western blot - Anti-Macrophage Inflammatory Protein 3 alpha antibody (AB106151)

All lanes:

Western blot - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab106151) at 1/1000 dilution

All lanes:

Mouse recombinant MIP3a protein (the immunogen) at 0.25 µg

Secondary

All lanes:

HRP Goat anti-Rabbit IgG at 1/40000 dilution

Predicted band size: 10 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Ccl20.

O89093

Specificity

<p>This Protein A purified antibody was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3α in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.</p>

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Reactivity data

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 0.1mL

Purification technique

Affinity purification Protein A

Purification notes

This Protein A purified antibody was heated to 56°C for 30 minutes.

Storage buffer

pH: 7.2 Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha) also known as CCL20 is a chemokine with a molecular mass of approximately 9 kDa. It plays a role in immune response by mediating chemotaxis of lymphocytes and dendritic cells. MIP-3 alpha is mainly expressed in liver lung and lymphoid organs including the lymph nodes and appendix. Its expression can be induced during inflammation in response to signaling molecules like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1β).

Biological function summary

MIP-3 alpha contributes to the immune system by recruiting immune cells to sites of inflammation or injury. It is not part of a larger protein complex. Its main function is binding to the CCR6 receptor on target cells. This interaction mediates the migration and activation of the immune cells critical for initiating the immune response by increasing cell surface adhesion and motility. By drawing immune cells MIP-3 alpha significantly influences the body's capacity to fight infections and regulate inflammatory processes.

Pathways

MIP-3 alpha is integral to the inflammatory response and chemokine signaling pathways. It is involved in the immune system's adaptive response through its interaction with CCR6. This pathway overlaps with other chemokines such as MIP-1 and MIP-2 which also aid in immune cell recruitment. Furthermore MIP-3 alpha’s engagement in these pathways facilitates intercellular communication during immune responses pivotal for maintaining homeostasis and immune surveillance.

MIP-3 alpha has associations with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. During these conditions elevated levels of MIP-3 alpha contribute to the recruitment of inflammatory cells which exacerbate symptoms. MIP-3 alpha also connects with other proteins such as TNF-alpha in these disease contexts enhancing inflammation and progression of these disorders. Targeting MIP-3 alpha and its associated pathways may provide therapeutic opportunities for alleviating these chronic inflammatory conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 20068036, PubMed : 9862452). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases (PubMed : 21376174). CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (By similarity). Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation (PubMed : 19050256). Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (PubMed : 24638065). Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed : 25122636). May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (PubMed : 10064080).

See full target information Ccl20

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35457188

2022

Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Shanshan Cui,Ling Hao,Wen Liu,Lijing Wang,Mengwei Ju,Wenjing Feng,Rong Xiao

Frontiers in oncology 11:646527 PubMed33842360

2021

The Collective Effect of MIP-3α and FL Promotes Dendritic Cell Function Within the Immune Microenvironment of Murine Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haichao Zhao,Changzhou Chen,Xidong Chen,Chuanli Yang,Donglin Zhang,Yanjun Li,Haoliang Zhao,Jiefeng He

Journal of immunological methods 133:181-90 PubMed2146321

1990

Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays.

Applications

Unspecified application

Species

Unspecified reactive species

M Oppermann,H Baumgarten,E Brandt,W Gottsleben,C Kurts,O Götze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Macrophage Inflammatory Protein 3 alpha antibody

Western blot - Anti-Macrophage Inflammatory Protein 3 alpha antibody (AB106151)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise