Rabbit Polyclonal Macrophage Inflammatory Protein 3 alpha antibody. Suitable for IHC-P, ICC, ELISA, WB, sELISA, IHC-FoFr, Neut, ICC/IF and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 43 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL20.
Images
Publications
Filter by
- International journal of oral science 15:562023Oral administration of Bifidobacterium breve improves anti-angiogenic drugs-derived oral mucosal wound healing impairment via upregulation of interleukin-10.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingxiang Li,Yuke Li,Qiao Qiao,Ning Zhao,Yuanning Yang,Lin Wang,Yifei Wang,Chuanbin Guo,Yuxing GuoPubMed 38072973
- Acta pharmaceutica Sinica. B 13:4748-47642023Preclinical and early clinical studies of a novel compound SYHA1813 that efficiently crosses the blood-brain barrier and exhibits potent activity against glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingqiang Liu,Zhengsheng Zhan,Zhuang Kang,Mengyuan Li,Yongcong Lv,Shenglan Li,Linjiang Tong,Fang Feng,Yan Li,Mengge Zhang,Yaping Xue,Yi Chen,Tao Zhang,Peiran Song,Yi Su,Yanyan Shen,Yiming Sun,Xinying Yang,Yi Chen,Shanyan Yao,Hanyu Yang,Caixia Wang,Meiyu Geng,Wenbin Li,Wenhu Duan,Hua Xie,Jian DingPubMed 38045044
- International journal of molecular sciences 24:2023Thymoquinone Inhibition of Chemokines in TNF-α-Induced Inflammatory and Metastatic Effects in Triple-Negative Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGetinet M Adinew,Samia Messeha,Equar Taka,Bereket Mochona,Kinfe K Redda,Karam F A SolimanPubMed 37373025
- Frontiers in immunology 14:10973832023Comprehensive protocols for culturing and molecular biological analysis of IBD patient-derived colon epithelial organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShreya Gopalakrishnan,Ingunn Bakke,Marianne Doré Hansen,Helene Kolstad Skovdahl,Atle van Beelen Granlund,Arne K Sandvik,Torunn BrulandPubMed 36911731
- Cancer science 114:1479-14902022Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20-CCR6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Kano,Kouji Izumi,Kaoru Hiratsuka,Ren Toriumi,Ryunosuke Nakagawa,Shuhei Aoyama,Taiki Kamijima,Takafumi Shimada,Renato Naito,Suguru Kadomoto,Hiroaki Iwamoto,Hiroshi Yaegashi,Shohei Kawaguchi,Takahiro Nohara,Kazuyoshi Shigehara,Yoshifumi Kadono,Yohei Saito,Kyoko Nakagawa-Goto,Kazuaki Yoshioka,Hiroki Nakata,Wen-Jye Lin,Atsushi MizokamiPubMed 36479732
- JCI insight 7:2022Iron accelerates Fusobacterium nucleatum-induced CCL8 expression in macrophages and is associated with colorectal cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaishi Yamane,Yohei Kanamori,Hiroshi Sawayama,Hiromu Yano,Akihiro Nita,Yudai Ohta,Hironori Hinokuma,Ayato Maeda,Akiko Iwai,Takashi Matsumoto,Mayuko Shimoda,Mayumi Niimura,Shingo Usuki,Noriko Yasuda-Yoshihara,Masato Niwa,Yoshifumi Baba,Takatsugu Ishimoto,Yoshihiro Komohara,Tomohiro Sawa,Tasuku Hirayama,Hideo Baba,Toshiro MoroishiPubMed 36136589
- Molecular cancer 21:1732022CircSMARCC1 facilitates tumor progression by disrupting the crosstalk between prostate cancer cells and tumor-associated macrophages via miR-1322/CCL20/CCR6 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Xie,Du-Jiang Fu,Zhi-Min Li,Dao-Jun Lv,Xian-Lu Song,Yu-Zhong Yu,Chong Wang,Kang-Jin Li,Baoqian Zhai,Jiacheng Wu,Ning-Han Feng,Shan-Chao ZhaoPubMed 36045408
- Cancer discovery 12:1336-13552022Dietary Lactobacillus-Derived Exopolysaccharide Enhances Immune-Checkpoint Blockade Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHirotaka Kawanabe-Matsuda,Kazuyoshi Takeda,Marie Nakamura,Seiya Makino,Takahiro Karasaki,Kazuhiro Kakimi,Megumi Nishimukai,Tatsukuni Ohno,Jumpei Omi,Kuniyuki Kano,Akiharu Uwamizu,Hideo Yagita,Ivo Gomperts Boneca,Gérard Eberl,Junken Aoki,Mark J Smyth,Ko OkumuraPubMed 35180303
- Frontiers in immunology 13:7647932022Keratin 17 Promotes T Cell Response in Allergic Contact Dermatitis by Upregulating C-C Motif Chemokine Ligand 20.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixin Luo,Zhenlai Zhu,Bing Li,Xiaocui Bai,Hui Fang,Pei Qiao,Jiaoling Chen,Chen Zhang,Dalong Zhi,Erle Dang,Gang WangPubMed 35178048
- Life (Basel, Switzerland) 11:2021A Plausible Proposition of CCL20-Related Mechanism in -Associated Oral Carcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDevi Prasad Mandal,Neeta Mohanty,Paresh Kumar Behera,Divya Gopinath,Sasmita Panda,Abdulaziz A Al-Kheraif,Darshan Devang Divakar,Sukumaran Anil,Swagatika PandaPubMed 34833094
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CCL20. Database link P78556
Reactivity data
Select an application| IHC-P | ICC | ELISA | WB | sELISA | IHC-FoFr | Neut | ICC/IF | |
|---|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Predicted | Expected | Expected | Expected | Expected | Expected |
| Mouse | Predicted | Expected | Predicted | Expected | Predicted | Expected | Expected | Expected |
| Rat | Predicted | Expected | Predicted | Expected | Predicted | Expected | Expected | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Expected | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect hMIP-3-alpha by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Species Rat | Dilution info - | Notes To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Species Human | Dilution info - | Notes To detect hMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect Human MIP-3α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271284 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3α. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 ug/ml of this antibody is required. |
Species Rat | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 ug/ml of this antibody is required. |
Species Human | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3-alpha (100 ng/ml), a concentration of 15.0 - 18.0 ug/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:11035086, PubMed:11352563, PubMed:20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases (PubMed:21376174). CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (PubMed:11352563, PubMed:9038201). Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (By similarity). C-terminal processed forms have been shown to be equally chemotactically active for leukocytes (PubMed:11035086). Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:23765988, PubMed:25122636). Inhibits proliferation of myeloid progenitors in colony formation assays (PubMed:9129037). May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (By similarity). Possesses antibacterial activity towards E.coli ATCC 25922 and S.aureus ATCC 29213 (PubMed:12149255).
Alternative names
LARC, MIP3A, SCYA20, CCL20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha
Recommended products
Rabbit Polyclonal Macrophage Inflammatory Protein 3 alpha antibody. Suitable for IHC-P, ICC, ELISA, WB, sELISA, IHC-FoFr, Neut, ICC/IF and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 43 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL20.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CCL20. Database link P78556
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] ab224188.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha) also known as CCL20 is a chemokine with a molecular mass of approximately 9 kDa. It plays a role in immune response by mediating chemotaxis of lymphocytes and dendritic cells. MIP-3 alpha is mainly expressed in liver lung and lymphoid organs including the lymph nodes and appendix. Its expression can be induced during inflammation in response to signaling molecules like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1β).
- Biological function summary
MIP-3 alpha contributes to the immune system by recruiting immune cells to sites of inflammation or injury. It is not part of a larger protein complex. Its main function is binding to the CCR6 receptor on target cells. This interaction mediates the migration and activation of the immune cells critical for initiating the immune response by increasing cell surface adhesion and motility. By drawing immune cells MIP-3 alpha significantly influences the body's capacity to fight infections and regulate inflammatory processes.
- Pathways
MIP-3 alpha is integral to the inflammatory response and chemokine signaling pathways. It is involved in the immune system's adaptive response through its interaction with CCR6. This pathway overlaps with other chemokines such as MIP-1 and MIP-2 which also aid in immune cell recruitment. Furthermore MIP-3 alpha’s engagement in these pathways facilitates intercellular communication during immune responses pivotal for maintaining homeostasis and immune surveillance.
- Associated diseases and disorders
MIP-3 alpha has associations with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. During these conditions elevated levels of MIP-3 alpha contribute to the recruitment of inflammatory cells which exacerbate symptoms. MIP-3 alpha also connects with other proteins such as TNF-alpha in these disease contexts enhancing inflammation and progression of these disorders. Targeting MIP-3 alpha and its associated pathways may provide therapeutic opportunities for alleviating these chronic inflammatory conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Macrophage Inflammatory Protein 3 alpha with ab9829 at 4 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue labelling Macrophage Inflammatory Protein 3 alpha with ab9829 at 4 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
Sandwich ELISA - Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab9829)
ab9829 detecting Macrophage Inflammatory Protein 3 alpha using sandwich ELISA
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com