JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242923

Anti-Macrophage inflammatory protein 5 antibody [EPR20024-133] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Macrophage inflammatory protein 5 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5

1 Images
Sandwich ELISA - Anti-Macrophage inflammatory protein 5 antibody [EPR20024-133] - BSA and Azide free (Detector) (AB242923)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Macrophage inflammatory protein 5 antibody [EPR20024-133] - BSA and Azide free (Detector) (AB242923)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab217774).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20024-133

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab242923 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MIP5. The recommended pair for sandwich ELISA is:
Capture: ab242678, Human MIP5 Capture Antibody (unconjugated)
Detector: ab242923, Human MIP5 Detector Antibody (unconjugated)
The reference range value is 15.6 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage inflammatory protein 5 (MIP-5) also known as CCL15 is a chemokine involved in immune system function. It plays an important role in recruiting and activating certain immune cells such as monocytes and lymphocytes. Its molecular mass is approximately 13.5 kDa. MIP-5 is produced mainly by monocytes and macrophages and can be found in the liver and several other tissues. This protein mediates inflammation by guiding immune cells to sites of infection or injury where they can perform immune defense activities.
Biological function summary

MIP-5 is involved in the chemotactic movement of immune cells a process where cells move in response to chemical stimuli. It is not part of a protein complex but interacts dynamically with cell surface receptors to induce cellular responses. MIP-5 triggers cells to release further signaling molecules increasing the inflammatory response's effectiveness. This chemokine creates a highly coordinated immune response by initiating a signaling cascade that helps maintain immune surveillance and response to pathogens.

Pathways

MIP-5 plays a role in the chemokine signaling pathway where it interacts with specific receptors that lead to cellular migration and activation. It also fits into the cytokine-cytokine receptor interaction pathway influencing a range of cellular responses tied to immune function. MIP-5 shares functional properties with other chemokines like MCP-1 (monocyte chemoattractant protein-1) which similarly attract immune cells and mediate immune responses.

MIP-5 is linked to inflammatory diseases and certain types of cancer. Elevated levels of MIP-5 have been observed in rheumatoid arthritis where it contributes to joint inflammation. It is also associated with hepatocellular carcinoma where it may influence tumor microenvironment and metastasis. In these conditions MIP-5 might interact with other inflammatory molecules such as IL-6 and TNF-alpha suggesting its role as a mediator of chronic inflammation and malignancy processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the CCL15.
See full target information CCL15
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB100598

Human MIP5 ELISA Kit (CCL15)

Sandwich ELISA - Human MIP5 ELISA Kit (CCL15) (AB100598)

  • 0
  • 0
Primary Antibodies

AB290643

Anti-CXCL9 antibody [EPR26512-118]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] (AB290643)

  • 0
  • 0
Primary Antibodies

AB242855

Anti-IL-17A antibody [EPR19941-89] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-17A antibody [EPR19941-89] - BSA and Azide free (Detector) (AB242855)

  • 0
  • 0
Primary Antibodies

AB214819

Anti-MCP1 antibody [EPR21025]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry - Anti-MCP1 antibody [EPR21025] (AB214819)

  • 0
  • 0
Primary Antibodies

AB242545

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (AB242545)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com