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Rabbit Recombinant Monoclonal MSP/MST1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

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Images

Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (AB242338), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Mouse
Expected

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Alternative names

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Rabbit Recombinant Monoclonal MSP/MST1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22184-140

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab242338 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MSP. The recommended pair for sandwich ELISA is:
Capture: Anti-Macrophage Stimulating Protein antibody [EPR22184-42] - BSA and Azide free (Capture) ab242337, Mouse MSP Capture Antibody (unconjugated)
Detector: ab242338, Mouse MSP Detector Antibody (unconjugated)
The reference range value is 312.5 - 20000 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

Activity summary

Macrophage Stimulating Protein (MSP) also known as hepatocyte growth factor-like protein is a cytokine that plays a role in immune response. MSP is a polypeptide composed of 711 amino acids and has a mass of approximately 80 kDa. This protein is mainly expressed in the liver and circulates in the blood as an inactive precursor. When it gets cleaved by serine proteases it transforms into an active form. The receptor for MSP is the RON receptor tyrosine kinase which mediates most of MSP's functions.

Biological function summary

MSP influences macrophage activation and migration. It is an important factor in inflammation and tissue repair processes. MSP does not commonly exist as a part of larger complexes but its activity is heavily dependent on its interaction with the RON receptor. Through this receptor MSP promotes the anti-inflammatory response and contributes to cell survival and proliferation. It also affects epithelial cell scattering and motility influencing wound healing.

Pathways

MSP is essential in both the PI3K-AKT and RAS-MAPK signaling pathways. These pathways are important for cell survival growth and proliferation. MSP's interaction with the RON receptor leads to the activation of these pathways which further links it with downstream proteins such as AKT1 and MAPK1. The cross-talk between these proteins results in biological effects that relate to cell movement and repair as well as immune modulation.

Associated diseases and disorders

MSP's role becomes evident in cancer and inflammatory disorders. In cancer MSP levels can influence tumor growth and metastasis due to its synergistic effects with proteins like MET another receptor tyrosine kinase. Increased MSP and RON activity can contribute to malignancy by promoting cellular invasion. In inflammatory conditions irregular MSP function can lead to chronic inflammation due to its impact on macrophage behavior interacting with cytokines like IL-6. Understanding MSP's roles in these conditions can help in developing targeted therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (ab242338), expandable thumbnail

    Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (ab242338)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse MSP ELISA Kit ab256390).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com