Rabbit Recombinant Monoclonal MSP/MST1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (AB242338), expandable thumbnail](https://content.abcam.com/products/images/macrophage-stimulating-protein-antibody-epr22184-140-bsa-and-azide-free-detector-ab242338--sandwich-elisa-img51622.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (AB242338)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Alternative names
Hepatocyte growth factor-like protein, Macrophage stimulatory protein, MSP, Hgfl, Mst1
Recommended products
Rabbit Recombinant Monoclonal MSP/MST1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Alternative names
Hepatocyte growth factor-like protein, Macrophage stimulatory protein, MSP, Hgfl, Mst1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22184-140
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab242338 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MSP. The recommended pair for sandwich ELISA is:
Capture: Anti-Macrophage Stimulating Protein antibody [EPR22184-42] - BSA and Azide free (Capture) ab242337, Mouse MSP Capture Antibody (unconjugated)
Detector: ab242338, Mouse MSP Detector Antibody (unconjugated)
The reference range value is 312.5 - 20000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- Activity summary
Macrophage Stimulating Protein (MSP) also known as hepatocyte growth factor-like protein is a cytokine that plays a role in immune response. MSP is a polypeptide composed of 711 amino acids and has a mass of approximately 80 kDa. This protein is mainly expressed in the liver and circulates in the blood as an inactive precursor. When it gets cleaved by serine proteases it transforms into an active form. The receptor for MSP is the RON receptor tyrosine kinase which mediates most of MSP's functions.
- Biological function summary
MSP influences macrophage activation and migration. It is an important factor in inflammation and tissue repair processes. MSP does not commonly exist as a part of larger complexes but its activity is heavily dependent on its interaction with the RON receptor. Through this receptor MSP promotes the anti-inflammatory response and contributes to cell survival and proliferation. It also affects epithelial cell scattering and motility influencing wound healing.
- Pathways
MSP is essential in both the PI3K-AKT and RAS-MAPK signaling pathways. These pathways are important for cell survival growth and proliferation. MSP's interaction with the RON receptor leads to the activation of these pathways which further links it with downstream proteins such as AKT1 and MAPK1. The cross-talk between these proteins results in biological effects that relate to cell movement and repair as well as immune modulation.
- Associated diseases and disorders
MSP's role becomes evident in cancer and inflammatory disorders. In cancer MSP levels can influence tumor growth and metastasis due to its synergistic effects with proteins like MET another receptor tyrosine kinase. Increased MSP and RON activity can contribute to malignancy by promoting cellular invasion. In inflammatory conditions irregular MSP function can lead to chronic inflammation due to its impact on macrophage behavior interacting with cytokines like IL-6. Understanding MSP's roles in these conditions can help in developing targeted therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (ab242338), expandable thumbnail](https://content.abcam.com/products/images/macrophage-stimulating-protein-antibody-epr22184-140-bsa-and-azide-free-detector-ab242338--sandwich-elisa-img51622.jpg "Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (ab242338)")
Sandwich ELISA - Anti-Macrophage Stimulating Protein antibody [EPR22184-140] - BSA and Azide free (Detector) (ab242338)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse MSP ELISA Kit ab256390).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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