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Rat Monoclonal Mouse Macrophage antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Mouse Macrophage protein.

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Publications

Key facts

Isotype

IgG2c

Host species

Rat

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Mouse Macrophage protein. The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PIHC-Fr
Mouse
Expected
Expected
Expected

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Recommended products

Rat Monoclonal Mouse Macrophage antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Mouse Macrophage protein.

Key facts

Isotype

IgG2c

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Mouse Macrophage protein. The exact immunogen used to generate this antibody is proprietary information.
Clone number

ER-HR3

Purification technique

Affinity purification Protein G

Specificity

This antibody is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are negative, up to 70% of circulating monocytes stain positive with this antibody. About 30% of bone marrow cells express the antigen. Other positive macrophages can be found in the splenic red pulp, in the mesentric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen.

Concentration
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Purification notes

Purified antibody in 0.2µm filtered solution.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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