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AB59697

Anti-Macrophages + Monocytes antibody [ER-HR3]

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(2 Publications)

Rat Monoclonal Mouse Macrophage antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Mouse Macrophage protein.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

ER-HR3

Isotype

IgG2c

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are negative, up to 70% of circulating monocytes stain positive with this antibody. About 30% of bone marrow cells express the antigen. Other positive macrophages can be found in the splenic red pulp, in the mesentric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen.

Reactivity data

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Product details

This antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified antibody in 0.2µm filtered solution.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Kidney international 83:84-92 PubMed22971995

2012

Tubular expression of heat-shock protein 27 inhibits fibrogenesis in obstructive nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Aparna Vidyasagar,Shannon R Reese,Omeed Hafez,Ling-Jin Huang,William F Swain,Lynn M Jacobson,Jose R Torrealba,Pierre-Emmanuel Chammas,Nancy A Wilson,Arjang Djamali

Journal of the American Society of Nephrology : JA 22:1863-75 PubMed21868496

2011

Valproic acid attenuates proteinuria and kidney injury.

Applications

IHC-P

Species

Mouse

Katrien Van Beneden,Caroline Geers,Marina Pauwels,Inge Mannaerts,Dierik Verbeelen,Leo A van Grunsven,Christiane Van den Branden
View all publications

Product promise

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