AB5783

Anti-MAD1L1/MAD1 antibody [9B10]

Name: Anti-MAD1L1/MAD1 antibody [9B10] (ab5783) | Abcam
Brand: Abcam Limited
SKU: AB5783
Price: 500 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Mouse Monoclonal MAD1L1/MAD1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAD1L1.

View Alternative Names

MAD1, TXBP181, MAD1L1, Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, Mitotic checkpoint MAD1 protein homolog, Tax-binding protein 181, MAD1-like protein 1, HsMAD1, hMAD1

1 Images

Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (AB5783)

Flow Cyt

Unknown

Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (AB5783)

Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9B10

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MAD1L1.

Q9Y6D9

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAD1L1 also known as MAD1 is a mitotic arrest deficient-like protein 1. It plays a critical role in cell cycle checkpoint control. MAD1L1 acts as an important component in the spindle assembly checkpoint (SAC) ensuring proper chromosome alignment and segregation during mitosis. This protein weighs roughly 85 kDa and locates primarily in the nucleus. It is expressed in various tissues with higher prevalence in rapidly dividing cells.

Biological function summary

MAD1L1 contributes to cellular homeostasis by preventing aneuploidy through its function in SAC. It participates in a larger protein complex with MAD2 another core component of SAC. Together MAD1L1 and MAD2 inhibit the anaphase-promoting complex/cyclosome (APC/C) delaying the onset of anaphase until all chromosomes align at the metaphase plate ensuring correct chromosome distribution to daughter cells.

Pathways

MAD1L1 influences the cell cycle and mitotic pathways. It interacts with other proteins like BUBR1 and CDC20 to maintain proper checkpoint function. These interactions place MAD1L1 at an important point in the pathway regulating cell division acting as both a sensor and a signal transducer to maintain genomic stability and prevent premature separation of sister chromatids.

MAD1L1 dysfunction can become associated with cancer and chromosomal instability disorders. Its aberrant expression or mutation can disrupt the accurate segregation of chromosomes leading to aneuploidy a hallmark in many types of cancer. MAD1L1 connects to key proteins like p53 and aurora kinases which also participate in maintaining genomic integrity and are often implicated in oncogenesis when mutated or dysregulated.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed : 10049595, PubMed : 20133940, PubMed : 29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed : 29162720).. Isoform 3. Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.

See full target information MAD1L1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Open biology 8: PubMed30257893

2018

Dynamic regulation of dynein localization revealed by small molecule inhibitors of ubiquitination enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Julie K Monda,Iain M Cheeseman

mBio 9: PubMed29739904

2018

CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into Epithelial and Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adam L Vanarsdall,Sarah R Pritchard,Todd W Wisner,Jing Liu,Ted S Jardetzky,David C Johnson

The Journal of cell biology 217:861-876 PubMed29362225

2018

The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Eleni Petsalaki,Maria Dandoulaki,George Zachos

Journal of cell science 127:3505-20 PubMed24938596

2014

Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function.

Applications

Unspecified application

Species

Unspecified reactive species

Kalpana Rajanala,Anshuk Sarkar,Gagan Deep Jhingan,Raina Priyadarshini,Manisha Jalan,Sagar Sengupta,Vinay Kumar Nandicoori

The Journal of cell biology 190:25-34 PubMed20624899

2010

Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex.

Applications

ICC/IF

Species

Human

Laura Hewitt,Anthony Tighe,Stefano Santaguida,Anne M White,Clifford D Jones,Andrea Musacchio,Stephen Green,Stephen S Taylor

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAD1L1/MAD1 antibody [9B10]

Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (AB5783)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise