AB126148

Anti-MAD1L1/MAD1 antibody

Name: Anti-MAD1L1/MAD1 antibody (ab126148) | Abcam
Brand: Abcam Limited
SKU: AB126148
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MAD1L1/MAD1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAD1L1 aa 400-700.

View Alternative Names

MAD1, TXBP181, MAD1L1, Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, Mitotic checkpoint MAD1 protein homolog, Tax-binding protein 181, MAD1-like protein 1, HsMAD1, hMAD1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody (AB126148)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody (AB126148)

ab126148, at 1/500 dilution, staining MAD1 in Formalin-fixed, Paraffin-embedded HBL435 xenograft by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-MAD1L1/MAD1 antibody (AB126148)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MAD1L1/MAD1 antibody (AB126148)

ab126148, at 1/500 dilution, staining MAD1 in Paraformaldehyde-fixed MCF-7 cells by Immunocytochemistry/Immunofluorescence.

Supplier Data

Immunoprecipitation - Anti-MAD1L1/MAD1 antibody (AB126148)

Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of ab126148.
Western blot analysis was performed using ab126148.
Anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-MAD1L1/MAD1 antibody (ab126148) at 5 µg

Lane 1:

HeLa whole cell extracts

Lane 2:

HeLa whole cell extracts + Control IgG

Lane 3:

HeLa whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG antibody

Predicted band size: 83 kDa

false

Supplier Data

Western blot - Anti-MAD1L1/MAD1 antibody (AB126148)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MAD1L1/MAD1 antibody (ab126148) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Lane 5:

A375 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAD1L1 aa 400-700. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6D9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Preform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0)", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAD1L1 also known as MAD1 is a mitotic arrest deficient-like protein 1. It plays a critical role in cell cycle checkpoint control. MAD1L1 acts as an important component in the spindle assembly checkpoint (SAC) ensuring proper chromosome alignment and segregation during mitosis. This protein weighs roughly 85 kDa and locates primarily in the nucleus. It is expressed in various tissues with higher prevalence in rapidly dividing cells.

Biological function summary

MAD1L1 contributes to cellular homeostasis by preventing aneuploidy through its function in SAC. It participates in a larger protein complex with MAD2 another core component of SAC. Together MAD1L1 and MAD2 inhibit the anaphase-promoting complex/cyclosome (APC/C) delaying the onset of anaphase until all chromosomes align at the metaphase plate ensuring correct chromosome distribution to daughter cells.

Pathways

MAD1L1 influences the cell cycle and mitotic pathways. It interacts with other proteins like BUBR1 and CDC20 to maintain proper checkpoint function. These interactions place MAD1L1 at an important point in the pathway regulating cell division acting as both a sensor and a signal transducer to maintain genomic stability and prevent premature separation of sister chromatids.

MAD1L1 dysfunction can become associated with cancer and chromosomal instability disorders. Its aberrant expression or mutation can disrupt the accurate segregation of chromosomes leading to aneuploidy a hallmark in many types of cancer. MAD1L1 connects to key proteins like p53 and aurora kinases which also participate in maintaining genomic integrity and are often implicated in oncogenesis when mutated or dysregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed : 10049595, PubMed : 20133940, PubMed : 29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed : 29162720).. Isoform 3. Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.

See full target information MAD1L1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

IUBMB life 69:540-552 PubMed28500689

2017

Blockade of hedgehog pathway is required for the protective effects of magnesium isoglycyrrhizinate against ethanol-induced hepatocyte steatosis and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfeng Lu,Wenxuan Xu,Jiangjuan Shao,Feng Zhang,Anping Chen,Shizhong Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAD1L1/MAD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody (AB126148)

Immunocytochemistry/ Immunofluorescence - Anti-MAD1L1/MAD1 antibody (AB126148)

Immunoprecipitation - Anti-MAD1L1/MAD1 antibody (AB126148)

Western blot - Anti-MAD1L1/MAD1 antibody (AB126148)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise