Rabbit Polyclonal MAD1L1/MAD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAD1L1 aa 400-700.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAD1L1 aa 400-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6D9
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Preform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0) |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:10049595, PubMed:20133940, PubMed:29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed:29162720). Isoform 3. Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.
Alternative names
MAD1, TXBP181, MAD1L1, Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, Mitotic checkpoint MAD1 protein homolog, Tax-binding protein 181, MAD1-like protein 1, HsMAD1, hMAD1
Recommended products
Rabbit Polyclonal MAD1L1/MAD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAD1L1 aa 400-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAD1L1 aa 400-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6D9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAD1L1 also known as MAD1 is a mitotic arrest deficient-like protein 1. It plays a critical role in cell cycle checkpoint control. MAD1L1 acts as an important component in the spindle assembly checkpoint (SAC) ensuring proper chromosome alignment and segregation during mitosis. This protein weighs roughly 85 kDa and locates primarily in the nucleus. It is expressed in various tissues with higher prevalence in rapidly dividing cells.
- Biological function summary
MAD1L1 contributes to cellular homeostasis by preventing aneuploidy through its function in SAC. It participates in a larger protein complex with MAD2 another core component of SAC. Together MAD1L1 and MAD2 inhibit the anaphase-promoting complex/cyclosome (APC/C) delaying the onset of anaphase until all chromosomes align at the metaphase plate ensuring correct chromosome distribution to daughter cells.
- Pathways
MAD1L1 influences the cell cycle and mitotic pathways. It interacts with other proteins like BUBR1 and CDC20 to maintain proper checkpoint function. These interactions place MAD1L1 at an important point in the pathway regulating cell division acting as both a sensor and a signal transducer to maintain genomic stability and prevent premature separation of sister chromatids.
- Associated diseases and disorders
MAD1L1 dysfunction can become associated with cancer and chromosomal instability disorders. Its aberrant expression or mutation can disrupt the accurate segregation of chromosomes leading to aneuploidy a hallmark in many types of cancer. MAD1L1 connects to key proteins like p53 and aurora kinases which also participate in maintaining genomic integrity and are often implicated in oncogenesis when mutated or dysregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-MAD1L1/MAD1 antibody (ab126148)
ab126148, at 1/500 dilution, staining MAD1 in Paraformaldehyde-fixed MCF-7 cells by Immunocytochemistry/Immunofluorescence.
Western blot - Anti-MAD1L1/MAD1 antibody (ab126148)
7.5% SDS PAGE
All lanes: Western blot - Anti-MAD1L1/MAD1 antibody (ab126148) at 1/1000 dilution
All lanes: MCF-7 whole cell lysate at 30 µg
Predicted band size: 101 kDa, 102 kDa, 76 kDa, 83 kDa, 84 kDa, 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody (ab126148)
ab126148, at 1/500 dilution, staining MAD1 in Formalin-fixed, Paraffin-embedded HBL435 xenograft by Immunohistochemistry.
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