AB70383

Anti-Mad2L1 antibody

Name: Anti-Mad2L1 antibody (ab70383) | Abcam
Brand: Abcam Limited
SKU: AB70383
Rating: 4 (2 reviews)

(2 Reviews)

(10 Publications)

Rabbit Polyclonal Mad2L1 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1.

View Alternative Names

MAD2, MAD2L1, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody (AB70383)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody (AB70383)

Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Mad2L1 with ab70383 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Mad2L1 antibody (AB70383)

MAD2 was immunoprecipitated from HEK-293T whole cell lysate (0.1 mg per IP reaction, 20% loaded) with ab70383 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70319 at 0.1 μg/mL.

Lane 1 : rabbit anti-MAD2 antibody IP in HEK-293T whole cell lysate.

Lane 2 : ab70383 IP in HEK-293T whole cell lysate.

Lane 3 : ab70383 IP in HEK-293T whole cell lysate

Lane 4 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-Mad2L1 antibody (ab70383)

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human MAD2L1. The exact immunogen used to generate this antibody is proprietary information.

Q13257

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Ferret": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mad2L1 also known as MAD2 or Kirk Mad2 is an important player in the mitotic checkpoint ensuring proper chromosomal segregation during cell division. It acts as a spindle assembly checkpoint protein weighing approximately 24.5 kDa. It is widely expressed in tissues with high proliferative activity including testis thymus and fetal liver. In these locations Mad2L1 binds to other mitotic checkpoint proteins to control the metaphase-anaphase transition.

Biological function summary

Mad2L1 serves as an essential component of the mitotic checkpoint complex. It forms a complex with other proteins such as Mad1 BubR1 and Cdc20 inhibiting the anaphase-promoting complex/cyclosome (APC/C). This interaction prevents the premature separation of sister chromatids by blocking the degradation of securin and cyclin B. These actions ensure that cells do not progress to anaphase until all chromosomes are correctly attached to the mitotic spindle.

Pathways

Mad2L1 interacts with proteins that regulate the spindle assembly checkpoint pathway and the cell cycle. It holds particular prominence within the mitotic spindle assembly checkpoint where it regulates progression from metaphase to anaphase through its interaction with proteins like Cdc20 and BubR1. Mad2L1 also plays a role in the DNA damage response cycle highlighting its importance in maintaining genomic stability.

Mad2L1 shows significant connections with cancer and chromosomal instability syndromes. Overexpression or dysfunction of Mad2L1 can lead to chromosomal instability contributing to carcinogenesis. Studies also suggest associations with BubR1 a protein whose expression frequently correlates with Mad2L1 in various cancers. These connections highlight Mad2L1 as a potential target for therapeutic intervention in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed : 15024386, PubMed : 29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed : 29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed : 10700282, PubMed : 11804586, PubMed : 15024386).

See full target information MAD2L1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e53408 PubMed37530743

2023

The cell cycle protein MAD2 facilitates endocytosis of the serotonin transporter in the neuronal soma.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Koban,Michael Freissmuth

Cell proliferation 56:e13384 PubMed36564861

2022

Selective utilization of non-homologous end-joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Crystal Lee,Jiyeon Leem,Jeong Su Oh

Nature communications 11:24 PubMed31911655

2020

The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Min Jang,Jenny F Nathans,Haiqing Fu,Christophe E Redon,Lisa M Jenkins,Bhushan L Thakur,Lőrinc S Pongor,Adrian M Baris,Jacob M Gross,Maura J OʹNeill,Fred E Indig,Steven D Cappell,Mirit I Aladjem

International journal of clinical and experimental 12:3357-3366 PubMed31934178

2019

The oncogenic role of TRIP13 in regulating proliferation, invasion, and cell cycle checkpoint in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Zhang,Yan Dong,Shaohuan Hao,Ying Tong,Qin Luo,Patiguli Aerxiding

Histochemistry and cell biology 152:207-215 PubMed31250100

2019

Zw10 is a spindle assembly checkpoint protein that regulates meiotic maturation in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuram Park,Jae-Sung Kim,Jeong Su Oh

Cell cycle (Georgetown, Tex.) 18:1254-1267 PubMed31111756

2019

Inhibition of neddylation causes meiotic arrest in mouse oocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Yang,Yimei Jin,Siying Fan,Xiaoling Liang,Jialin Jia,Zhongzhou Tan,Tao Huang,Yuan Li,Teng Ma,Mo Li

Oncotarget 8:77649-77672 PubMed29100415

2017

The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1.

Applications

Unspecified application

Species

Unspecified reactive species

Miyoung Lee,Yainyrette Rivera-Rivera,Carlos S Moreno,Harold I Saavedra

Journal of cell science 127:3505-20 PubMed24938596

2014

Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function.

Applications

Unspecified application

Species

Unspecified reactive species

Kalpana Rajanala,Anshuk Sarkar,Gagan Deep Jhingan,Raina Priyadarshini,Manisha Jalan,Sagar Sengupta,Vinay Kumar Nandicoori

The Journal of biological chemistry 289:8182-93 PubMed24509855

2014

Interplay between the DNA damage proteins MDC1 and ATM in the regulation of the spindle assembly checkpoint.

Applications

ICC/IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Yifat Eliezer,Liron Argaman,Maya Kornowski,Maayan Roniger,Michal Goldberg

World journal of gastroenterology 19:2913-20 PubMed23704824

2013

Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma.

Applications

Species

Human

Wei Sun,Wei-Wei Dong,Lin-Lin Mao,Wen-Mei Li,Jian-Tao Cui,Rui Xing,You-Yong Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mad2L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody (AB70383)

Immunoprecipitation - Anti-Mad2L1 antibody (AB70383)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise