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Rabbit Polyclonal Mad2L1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1.

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Images

Immunoprecipitation - Anti-Mad2L1 antibody (AB70385), expandable thumbnail
  • Western blot - Anti-Mad2L1 antibody (AB70385), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human MAD2L1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13257

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Chimpanzee
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Cow
Predicted
Predicted
Elephant
Predicted
Predicted
Gorilla
Predicted
Predicted
Guinea pig
Predicted
Predicted
Horse
Predicted
Predicted
Orangutan
Predicted
Predicted
Pig
Predicted
Predicted
Rabbit
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:15024386, PubMed:29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386).

Alternative names

Recommended products

Rabbit Polyclonal Mad2L1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human MAD2L1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13257
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Mad2L1 also known as MAD2 or Kirk Mad2 is an important player in the mitotic checkpoint ensuring proper chromosomal segregation during cell division. It acts as a spindle assembly checkpoint protein weighing approximately 24.5 kDa. It is widely expressed in tissues with high proliferative activity including testis thymus and fetal liver. In these locations Mad2L1 binds to other mitotic checkpoint proteins to control the metaphase-anaphase transition.

Biological function summary

Mad2L1 serves as an essential component of the mitotic checkpoint complex. It forms a complex with other proteins such as Mad1 BubR1 and Cdc20 inhibiting the anaphase-promoting complex/cyclosome (APC/C). This interaction prevents the premature separation of sister chromatids by blocking the degradation of securin and cyclin B. These actions ensure that cells do not progress to anaphase until all chromosomes are correctly attached to the mitotic spindle.

Pathways

Mad2L1 interacts with proteins that regulate the spindle assembly checkpoint pathway and the cell cycle. It holds particular prominence within the mitotic spindle assembly checkpoint where it regulates progression from metaphase to anaphase through its interaction with proteins like Cdc20 and BubR1. Mad2L1 also plays a role in the DNA damage response cycle highlighting its importance in maintaining genomic stability.

Associated diseases and disorders

Mad2L1 shows significant connections with cancer and chromosomal instability syndromes. Overexpression or dysfunction of Mad2L1 can lead to chromosomal instability contributing to carcinogenesis. Studies also suggest associations with BubR1 a protein whose expression frequently correlates with Mad2L1 in various cancers. These connections highlight Mad2L1 as a potential target for therapeutic intervention in oncology.

Product promise

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2 product images

  • Immunoprecipitation - Anti-Mad2L1 antibody (ab70385), expandable thumbnail

    Immunoprecipitation - Anti-Mad2L1 antibody (ab70385)

    MAD2 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70385 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70385 at 1 μg/mL.

    Lane 1: ab70385 IP in HeLa whole cell lysate.

    Lane 2: ab70385 IP in HeLa whole cell lysate.

    Lane 3: rabbit anti-MAD2 antibody IP in HeLa whole cell lysate.

    Lane 4: Control IgG in HeLa whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 minutes

    All lanes: Immunoprecipitation - Anti-Mad2L1 antibody (ab70385)

    Predicted band size: 24 kDa

  • Western blot - Anti-Mad2L1 antibody (ab70385), expandable thumbnail

    Western blot - Anti-Mad2L1 antibody (ab70385)

    All lanes: Western blot - Anti-Mad2L1 antibody (ab70385) at 0.1 µg/mL

    Lane 1: HeLa whole cell lysate at 50 µg

    Lane 2: HEK293T whole cell lysate at 50 µg

    Lane 3: Jurkat whole cell lysate at 50 µg

    Lane 4: TCMK-1 whole cell lysate at 50 µg

    Lane 5: NIH/3T3 whole cell lysate at 50 µg

    Predicted band size: 24 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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