Rabbit Polyclonal Mad2L1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Liquid
Polyclonal
IP | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Chimpanzee | Predicted | Predicted |
Chinese hamster | Predicted | Predicted |
Cow | Predicted | Predicted |
Elephant | Predicted | Predicted |
Gorilla | Predicted | Predicted |
Guinea pig | Predicted | Predicted |
Horse | Predicted | Predicted |
Orangutan | Predicted | Predicted |
Pig | Predicted | Predicted |
Rabbit | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
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Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:15024386, PubMed:29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386).
MAD2, MAD2L1, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1
Rabbit Polyclonal Mad2L1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1.
IgG
Rabbit
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Mad2L1 also known as MAD2 or Kirk Mad2 is an important player in the mitotic checkpoint ensuring proper chromosomal segregation during cell division. It acts as a spindle assembly checkpoint protein weighing approximately 24.5 kDa. It is widely expressed in tissues with high proliferative activity including testis thymus and fetal liver. In these locations Mad2L1 binds to other mitotic checkpoint proteins to control the metaphase-anaphase transition.
Mad2L1 serves as an essential component of the mitotic checkpoint complex. It forms a complex with other proteins such as Mad1 BubR1 and Cdc20 inhibiting the anaphase-promoting complex/cyclosome (APC/C). This interaction prevents the premature separation of sister chromatids by blocking the degradation of securin and cyclin B. These actions ensure that cells do not progress to anaphase until all chromosomes are correctly attached to the mitotic spindle.
Mad2L1 interacts with proteins that regulate the spindle assembly checkpoint pathway and the cell cycle. It holds particular prominence within the mitotic spindle assembly checkpoint where it regulates progression from metaphase to anaphase through its interaction with proteins like Cdc20 and BubR1. Mad2L1 also plays a role in the DNA damage response cycle highlighting its importance in maintaining genomic stability.
Mad2L1 shows significant connections with cancer and chromosomal instability syndromes. Overexpression or dysfunction of Mad2L1 can lead to chromosomal instability contributing to carcinogenesis. Studies also suggest associations with BubR1 a protein whose expression frequently correlates with Mad2L1 in various cancers. These connections highlight Mad2L1 as a potential target for therapeutic intervention in oncology.
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Terms & Conditions.
MAD2 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70385 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70385 at 1 μg/mL.
Lane 1: ab70385 IP in HeLa whole cell lysate.
Lane 2: ab70385 IP in HeLa whole cell lysate.
Lane 3: rabbit anti-MAD2 antibody IP in HeLa whole cell lysate.
Lane 4: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
All lanes: Immunoprecipitation - Anti-Mad2L1 antibody (ab70385)
Predicted band size: 24 kDa
All lanes: Western blot - Anti-Mad2L1 antibody (ab70385) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HEK293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK-1 whole cell lysate at 50 µg
Lane 5: NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 24 kDa
Exposure time: 3min
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