Rabbit Polyclonal Mad2L1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1 aa 150 to C-terminus.
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Publications
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- The EMBO journal 42:e1115872023Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Tovini,Sarah C Johnson,Molly A Guscott,Alexander M Andersen,Diana Carolina Johanna Spierings,René Wardenaar,Floris Foijer,Sarah E McClellandPubMed 37063065
- Journal of breast cancer 25:178-1922022Polo-Like Kinase 1 Regulates Chromosomal Instability and Paclitaxel Resistance in Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingji Quan,Yumi Oh,Sung-Yup Cho,Ju Hee Kim,Hyeong-Gon MoonPubMed 35775700
- Cell biology international 46:840-8482022Targeting of MAD2L1 by miR-515-5p involves the regulation of cell cycle arrest and apoptosis of colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Ding,Qingyan Fu,Weixing Chen,Linjie Chen,Qingjun Zeng,Sanjun Zhang,Linfang HePubMed 35143103
- Cancer cell international 21:6342021Bioinformatics prediction and experimental verification identify MAD2L1 and CCNB2 as diagnostic biomarkers of rhabdomyosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Xia,Lian Meng,Zhijuan Zhao,Yujun Li,Hao Wen,Hao Sun,Tiantian Zhang,Jingxian Wei,Feng Li,Chunxia LiuPubMed 34838000
- Biochimica et biophysica acta. Molecular cell research 1868:1189292020Tank Binding Kinase 1 modulates spindle assembly checkpoint components to regulate mitosis in breast and lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeenu Maan,Neha Jaiswal Agrawal,Jaya Padmanabhan,Christelle Colin Leitzinger,Yainyrette Rivera-Rivera,Harold I Saavedra,Srikumar P ChellappanPubMed 33310066
- Computational and mathematical methods in medicine 2020:29535982020miR-139-5p Inhibits Lung Adenocarcinoma Cell Proliferation, Migration, and Invasion by Targeting MAD2L1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianfeng Li et. al.PubMed 33204298
- Oncogenesis 9:432020MiR-93 is related to poor prognosis in pancreatic cancer and promotes tumor progression by targeting microtubule dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Vila-Navarro et. al.PubMed 32366853
- Cell reports 23:852-8652018USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.Applications:Unspecified applicationReactive species:HumanAgnieszka Skowyra,Lindsey A Allan,Adrian T Saurin,Paul R ClarkePubMed 29669289
- Nature communications 7:101512016Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaining Zhou et. al.PubMed 26743940
- PloS one 8:e664322013Chemokine localization in bronchial angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Grazia Perino et. al.PubMed 23776670
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAD2L1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13257
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:15024386, PubMed:29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386).
Alternative names
MAD2, MAD2L1, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1
Recommended products
Rabbit Polyclonal Mad2L1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L1 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAD2L1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13257
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mad2L1 also known as MAD2 or Kirk Mad2 is an important player in the mitotic checkpoint ensuring proper chromosomal segregation during cell division. It acts as a spindle assembly checkpoint protein weighing approximately 24.5 kDa. It is widely expressed in tissues with high proliferative activity including testis thymus and fetal liver. In these locations Mad2L1 binds to other mitotic checkpoint proteins to control the metaphase-anaphase transition.
- Biological function summary
Mad2L1 serves as an essential component of the mitotic checkpoint complex. It forms a complex with other proteins such as Mad1 BubR1 and Cdc20 inhibiting the anaphase-promoting complex/cyclosome (APC/C). This interaction prevents the premature separation of sister chromatids by blocking the degradation of securin and cyclin B. These actions ensure that cells do not progress to anaphase until all chromosomes are correctly attached to the mitotic spindle.
- Pathways
Mad2L1 interacts with proteins that regulate the spindle assembly checkpoint pathway and the cell cycle. It holds particular prominence within the mitotic spindle assembly checkpoint where it regulates progression from metaphase to anaphase through its interaction with proteins like Cdc20 and BubR1. Mad2L1 also plays a role in the DNA damage response cycle highlighting its importance in maintaining genomic stability.
- Associated diseases and disorders
Mad2L1 shows significant connections with cancer and chromosomal instability syndromes. Overexpression or dysfunction of Mad2L1 can lead to chromosomal instability contributing to carcinogenesis. Studies also suggest associations with BubR1 a protein whose expression frequently correlates with Mad2L1 in various cancers. These connections highlight Mad2L1 as a potential target for therapeutic intervention in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Mad2L1 antibody (ab97777)
All lanes: Western blot - Anti-Mad2L1 antibody (ab97777) at 1/1000 dilution
Lane 1: HepG2 whole cell lysate at 30 µg
Lane 2: MOLT4 whole cell lysate at 30 µg
Lane 3: Raji whole cell lysate at 30 µg
Predicted band size: 24 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Mad2L1 antibody (ab97777)
Immunofluorescence analysis of Mad2L1 in Human osteosarcoma cell line U2OS, using ab97777 at 1/1000 dilution.
1: Mad2L1
2: ACA
3: merged with DAPI
Cells were fixed with 4% PFA/PBS for 15min, RT and blocked with 2.5% BSA/PBS.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody (ab97777)
Immunohistochemical analysis of Mad2L1 in paraffin embedded DLD-1 xenograft, using ab97777 at a 1/500 dilution.
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