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AB171084

Anti-Mad2L1 antibody [EPR9852]

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(1 Publication)

Rabbit Recombinant Monoclonal Mad2L1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAD2, MAD2L1, Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1

2 Images
Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)
  • WB

Supplier Data

Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)

All lanes:

Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 24 kDa

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Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)
  • WB

Lab

Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)

Lanes 1 - 4 : Merged signal (red and green). Green - ab171084 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab171084 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab171084 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

MAD2L1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

A431 whole cell lysate at 20 µg

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9852

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: BSA, Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mad2L1 also known as MAD2 or Kirk Mad2 is an important player in the mitotic checkpoint ensuring proper chromosomal segregation during cell division. It acts as a spindle assembly checkpoint protein weighing approximately 24.5 kDa. It is widely expressed in tissues with high proliferative activity including testis thymus and fetal liver. In these locations Mad2L1 binds to other mitotic checkpoint proteins to control the metaphase-anaphase transition.
Biological function summary

Mad2L1 serves as an essential component of the mitotic checkpoint complex. It forms a complex with other proteins such as Mad1 BubR1 and Cdc20 inhibiting the anaphase-promoting complex/cyclosome (APC/C). This interaction prevents the premature separation of sister chromatids by blocking the degradation of securin and cyclin B. These actions ensure that cells do not progress to anaphase until all chromosomes are correctly attached to the mitotic spindle.

Pathways

Mad2L1 interacts with proteins that regulate the spindle assembly checkpoint pathway and the cell cycle. It holds particular prominence within the mitotic spindle assembly checkpoint where it regulates progression from metaphase to anaphase through its interaction with proteins like Cdc20 and BubR1. Mad2L1 also plays a role in the DNA damage response cycle highlighting its importance in maintaining genomic stability.

Mad2L1 shows significant connections with cancer and chromosomal instability syndromes. Overexpression or dysfunction of Mad2L1 can lead to chromosomal instability contributing to carcinogenesis. Studies also suggest associations with BubR1 a protein whose expression frequently correlates with Mad2L1 in various cancers. These connections highlight Mad2L1 as a potential target for therapeutic intervention in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed : 15024386, PubMed : 29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed : 29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed : 10700282, PubMed : 11804586, PubMed : 15024386).
See full target information MAD2L1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 13:2293-2300 PubMed35517421

2022

PDZRN4 suppresses tumorigenesis and androgen therapy-resistance in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Jin,Lielin Wu,Gang Zhang,Bo Yang,Bisong Zhu
View all publications
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Product promise

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