Anti-Mad2L1 antibody [EPR9853(B)] - BSA and Azide free

• WB

Unknown

Western blot - Anti-Mad2L1 antibody [EPR9853(B)] - BSA and Azide free (AB248948)

This data was developed using ab150371, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Mad2L1 antibody [EPR9853(B)] (<a href='/en-us/products/primary-antibodies/mad2l1-antibody-epr9853b-ab150371'>ab150371</a>) at 1/10000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Lane 3:

Raji cell lysates at 10 µg

Lane 4:

A431 cell lysates at 10 µg

Predicted band size: 24 kDa

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• WB

Lab

Western blot - Anti-Mad2L1 antibody [EPR9853(B)] - BSA and Azide free (AB248948)

This data was developed using ab150371, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab150371 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab150371 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab150371 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Mad2L1 antibody [EPR9853(B)] (<a href='/en-us/products/primary-antibodies/mad2l1-antibody-epr9853b-ab150371'>ab150371</a>) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

MAD2L1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

A431 whole cell lysate at 20 µg

Predicted band size: 24 kDa

Observed band size: 24 kDa

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