AB115622

Anti-Mad2L2/REV7 antibody

Name: Anti-Mad2L2/REV7 antibody (ab115622) | Abcam
Brand: Abcam Limited
SKU: AB115622
Rating: 5 (3 reviews)

(3 Reviews)

(2 Publications)

Rabbit Polyclonal Mad2L2/REV7 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MAD2L2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MAD2B, REV7, MAD2L2, Mitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, REV7 homolog, MAD2-like protein 2, hREV7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (AB115622)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (AB115622)

ab115622, at 10μg/ml, staining Mad2L2/REV7 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

AbReview36708****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (AB115622)

ab115622 staining Mad2L2/REV7 in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the Hope Technique and blocked for 15 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MAD2L2 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UI95

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Mad2L2 also known as REV7 plays important roles in cellular processes. It has a molecular weight of about 26 kDa and is expressed in various tissues including the testis and placenta. Mad2L2 is an important component of the DNA damage response functioning mainly in the regulation of mitosis and repair of DNA double-strand breaks. It acts as a regulatory subunit and inhibitor of the translesion DNA synthesis (TLS) polymerase complex influencing processes linked to DNA fidelity.

Biological function summary

The protein is important for maintaining genome integrity by participating in the Fanconi anemia (FA) pathway ensuring proper DNA repair. Mad2L2 forms an important part of the homologous recombination repair system and plays a role in the recruitment of additional repair factors. It is often seen as a part of the shieldin complex which works to protect DNA ends during repair. This complex safeguarding allows it to prevent inappropriate activity during the DNA repair process.

Pathways

The involvement of Mad2L2 highlights its critical role in DNA repair mechanisms specifically homologous recombination and non-homologous end joining (NHEJ). It interacts with proteins such as 53BP1 and RIF1 within these pathways to mediate DNA repair. Such interaction is essential for choosing the correct repair pathway based on cellular needs. Mad2L2's function within the pathway guarantees the accurate resolution and repair of DNA lesions.

The protein's relevance underlines its connection to cancer and genetic disorders like Fanconi anemia. Mutations or irregularities in Mad2L2 can contribute to genomic instability leading to cancer development. In particular its interaction with the BRCA1 and BRCA2 proteins is significant; disruptions in these interactions link to increased cancer risk. This connection with tumor suppression pathways makes Mad2L2 an interesting target for therapeutic strategies in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Adapter protein able to interact with different proteins and involved in different biological processes (PubMed : 11459825, PubMed : 11459826, PubMed : 17296730, PubMed : 17719540, PubMed : 19443654, PubMed : 29656893). Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis (PubMed : 20164194, PubMed : 23143872). Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions (PubMed : 20164194). Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs) (PubMed : 29656893). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection (PubMed : 29656893). Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres (PubMed : 29656893). May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1 (PubMed : 17296730). Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle (PubMed : 11459825, PubMed : 17719540). Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (PubMed : 19443654).

See full target information MAD2L2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:1138 PubMed34880209

2021

FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Ping Wang,Shi-Qi Wu,Shi-Hui Huang,Yi-Xuan Tang,Liu-Qiong Meng,Feng Liu,Qi-Hua Zhu,Yun-Gen Xu

Cancer cell 37:157-167.e6 PubMed32004442

2020

EZH2 Inhibition Sensitizes CARM1-High, Homologous Recombination Proficient Ovarian Cancers to PARP Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey Karakashev,Takeshi Fukumoto,Bo Zhao,Jianhuang Lin,Shuai Wu,Nail Fatkhutdinov,Pyoung-Hwa Park,Galina Semenova,Stephanie Jean,Mark G Cadungog,Mark E Borowsky,Andrew V Kossenkov,Qin Liu,Rugang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mad2L2/REV7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (AB115622)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (AB115622)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise