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Rabbit Recombinant Monoclonal Mad2L2/REV7 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (AB232620), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (AB232620), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (AB232620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (AB232620), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Expected
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

1 product for Alternative Product

Target data

Function

Adapter protein able to interact with different proteins and involved in different biological processes (PubMed:11459825, PubMed:11459826, PubMed:17296730, PubMed:17719540, PubMed:19443654, PubMed:29656893). Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis (PubMed:20164194). Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions (PubMed:20164194). Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs) (PubMed:29656893). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection (PubMed:29656893). Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres (PubMed:29656893). May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1 (PubMed:17296730). Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle (PubMed:11459825, PubMed:17719540). Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (PubMed:19443654).

Alternative names

MAD2B, REV7, MAD2L2, MAD2B, REV7, Mitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, REV7 homolog, MAD2-like protein 2, hREV7

Recommended products

Rabbit Recombinant Monoclonal Mad2L2/REV7 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13657

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab232620 is the carrier-free version of Anti-Mad2L2/REV7 antibody [EPR13657] ab180579.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein Mad2L2 also known as REV7 plays important roles in cellular processes. It has a molecular weight of about 26 kDa and is expressed in various tissues including the testis and placenta. Mad2L2 is an important component of the DNA damage response functioning mainly in the regulation of mitosis and repair of DNA double-strand breaks. It acts as a regulatory subunit and inhibitor of the translesion DNA synthesis (TLS) polymerase complex influencing processes linked to DNA fidelity.

Biological function summary

The protein is important for maintaining genome integrity by participating in the Fanconi anemia (FA) pathway ensuring proper DNA repair. Mad2L2 forms an important part of the homologous recombination repair system and plays a role in the recruitment of additional repair factors. It is often seen as a part of the shieldin complex which works to protect DNA ends during repair. This complex safeguarding allows it to prevent inappropriate activity during the DNA repair process.

Pathways

The involvement of Mad2L2 highlights its critical role in DNA repair mechanisms specifically homologous recombination and non-homologous end joining (NHEJ). It interacts with proteins such as 53BP1 and RIF1 within these pathways to mediate DNA repair. Such interaction is essential for choosing the correct repair pathway based on cellular needs. Mad2L2's function within the pathway guarantees the accurate resolution and repair of DNA lesions.

Associated diseases and disorders

The protein's relevance underlines its connection to cancer and genetic disorders like Fanconi anemia. Mutations or irregularities in Mad2L2 can contribute to genomic instability leading to cancer development. In particular its interaction with the BRCA1 and BRCA2 proteins is significant; disruptions in these interactions link to increased cancer risk. This connection with tumor suppression pathways makes Mad2L2 an interesting target for therapeutic strategies in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620), expandable thumbnail

    Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Immunoprecipitation of Hela cells labeling Mad2L2/REV7 using Anti-Mad2L2/REV7 antibody [EPR13657] ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mad2L2/REV7 antibody [EPR13657] ab180579).

    All lanes: Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] (Anti-Mad2L2/REV7 antibody [EPR13657] ab180579)

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2/REV7 with Anti-PGD antibody [EPR6565] ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mad2L2/REV7 antibody [EPR13657] ab180579).

  • Flow Cytometry (Intracellular) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Intracellular flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2/REV7 with Anti-Mad2L2/REV7 antibody [EPR13657] ab180579 at 1/70 dilution andGoat anti rabbit IgG (FITC) at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mad2L2/REV7 antibody [EPR13657] ab180579).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Immunohistochemical staining of paraffin-embedded human ovarian carcinoma tissue labeling Mad2L2/REV7 using Anti-Mad2L2/REV7 antibody [EPR13657] ab180579 at a 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mad2L2/REV7 antibody [EPR13657] ab180579).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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