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AB80680

Anti-MAdCAM1 antibody [AP-MAB0842]

5

(1 Review)

|

(3 Publications)

Rat Monoclonal MAdCAM1 antibody. Carrier free. Suitable for IP, Flow Cyt, IHC-P, FuncS (Neut/Block), IHC-Fr and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Madcam1 protein.

View Alternative Names

Mucosal addressin cell adhesion molecule 1, MAdCAM-1, mMAdCAM-1, Madcam1

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

AP-MAB0842

Isotype

IgG2

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt, IHC-P, FuncS (Neut/Block), IHC-Fr, IP

applications

Immunogen

Cell preparation containing Madcam1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61826

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Alternative Products

Primary Antibodies

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Anti-MAdCAM1 antibody [314G8]

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Primary Antibodies

AB198277

Anti-MAdCAM1 antibody

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Primary Antibodies

AB214198

Anti-MAdCAM1 antibody

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Primary Antibodies

AB237020

Anti-MAdCAM1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody (AB237020)

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Complementary Products

Assay Kits

AB315431

Human MAdCAM1 ELISA Kit- extracellular

SimpleStep

Sandwich ELISA - Human MAdCAM1 ELISA Kit- extracellular (AB315431)

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Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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  • 105
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Endotoxin Level: Extremely low level of LPS (< 0.002EU/μg IgG).

ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).

ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
The IgG fraction of culture supernatant was purified by Protein Aaffinity chromatography.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAdCAM-1 also known as mucosal addressin cell adhesion molecule 1 or mucosal tag is a protein with a molecular mass of approximately 60 kDa. The MAdCAM-1 protein is expressed prominently on high endothelial venules within mucosal tissues such as the gut and the colon. Additionally it also appears in the endothelial cells of lymphoid tissues. Another name for it is MECA-79 highlighting its recognition by the MECA-79 antibody. Mucosal tags label regions where MAdCAM-1 assists in cell adhesion processes.
Biological function summary

MAdCAM-1 facilitates lymphocyte homing to mucosal tissues. It plays a part in the adhesive interactions between endothelial cells and lymphocytes as a component of the immune surveillance system enabling T cells and B cells to move into areas where they are needed. MAdCAM-1 forms complexes with integrins notably alpha4beta7 which are integral to its adhesion functions. Through its role in guiding lymphocytes it influences immune response and inflammation in mucosal environments.

Pathways

MAdCAM-1 contributes importantly to the trafficking of immune cells. It is involved in the regulation of cell adhesion and migration pathways. The protein interacts extensively with the integrin family especially integrin alpha4beta7 which facilitates lymphocyte entry into the intestinal wall. Additionally it works with chemokine receptors and cytokines that further influence cell movement and homing to specific tissues. This integrative role reinforces its importance in immune response and mucosal immunity.

MAdCAM-1 links closely to inflammatory diseases such as Crohn's disease and ulcerative colitis. Its expression is upregulated in these inflammatory conditions which leads to increased lymphocyte accumulation in the gut. This condition exacerbates the inflammation and tissue damage characteristic of these disorders. MECA-79 a monoclonal antibody targets MAdCAM-1 to aid therapeutic approaches in mitigating inflammation driven by lymphocyte infiltration. Understanding MAdCAM-1 and its function offers potential pathways to target and treat these chronic inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
See full target information Madcam1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:2020-2034 PubMed39528700

2024

Specialized post-arterial capillaries facilitate adult bone remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Vishal Mohanakrishnan,Kishor K Sivaraj,Hyun-Woo Jeong,Esther Bovay,Backialakshmi Dharmalingam,M Gabriele Bixel,Van Vuong Dinh,Milena Petkova,Isidora Paredes Ugarte,Yi-Tong Kuo,Malarvizhi Gurusamy,Brian Raftrey,Nelson Tsz Long Chu,Soumyashree Das,Pamela E Rios Coronado,Martin Stehling,Lars Sävendahl,Andrei S Chagin,Taija Mäkinen,Kristy Red-Horse,Ralf H Adams

Mucosal immunology 14:479-490 PubMed33004979

2020

Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Y Gao,Colin T Shearn,David J Orlicky,Kayla D Battista,Erica E Alexeev,Ian M Cartwright,Jordi M Lanis,Rachael E Kostelecky,Cynthia Ju,Sean P Colgan,Blair P Fennimore

Experimental and therapeutic medicine 20:1637-1643 PubMed32765677

2020

miR-365b regulates the development of non-small cell lung cancer via GALNT4.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xing,Xiaodong Hong,Liang Chang,Ping Ren,Hong Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com