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Rabbit Recombinant Monoclonal MAdCAM1 antibody. Suitable for IHC-P, ICC/IF, mIHC and reacts with Mouse, Rat, Transfected cell line - Mouse samples.


Images

Multiplex immunohistochemistry - Anti-MAdCAM1 antibody [EPR27223-58] (AB309487), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-MAdCAM1 antibody [EPR27223-58] (AB309487), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody [EPR27223-58] (AB309487), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody [EPR27223-58] (AB309487), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (AB309487), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow CytIPmIHC
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Expected
Not recommended
Not recommended
Tested
Transfected cell line - Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Transfected cell line - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Transfected cell line - Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell line - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Mouse
Dilution info
-
Notes

-

Target data

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Alternative names

Mucosal addressin cell adhesion molecule 1, MAdCAM-1, mMAdCAM-1, Madcam1

Recommended products

Rabbit Recombinant Monoclonal MAdCAM1 antibody. Suitable for IHC-P, ICC/IF, mIHC and reacts with Mouse, Rat, Transfected cell line - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27223-58
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MAdCAM-1 also known as mucosal addressin cell adhesion molecule 1 or mucosal tag is a protein with a molecular mass of approximately 60 kDa. The MAdCAM-1 protein is expressed prominently on high endothelial venules within mucosal tissues such as the gut and the colon. Additionally it also appears in the endothelial cells of lymphoid tissues. Another name for it is MECA-79 highlighting its recognition by the MECA-79 antibody. Mucosal tags label regions where MAdCAM-1 assists in cell adhesion processes.

Biological function summary

MAdCAM-1 facilitates lymphocyte homing to mucosal tissues. It plays a part in the adhesive interactions between endothelial cells and lymphocytes as a component of the immune surveillance system enabling T cells and B cells to move into areas where they are needed. MAdCAM-1 forms complexes with integrins notably alpha4beta7 which are integral to its adhesion functions. Through its role in guiding lymphocytes it influences immune response and inflammation in mucosal environments.

Pathways

MAdCAM-1 contributes importantly to the trafficking of immune cells. It is involved in the regulation of cell adhesion and migration pathways. The protein interacts extensively with the integrin family especially integrin alpha4beta7 which facilitates lymphocyte entry into the intestinal wall. Additionally it works with chemokine receptors and cytokines that further influence cell movement and homing to specific tissues. This integrative role reinforces its importance in immune response and mucosal immunity.

Associated diseases and disorders

MAdCAM-1 links closely to inflammatory diseases such as Crohn's disease and ulcerative colitis. Its expression is upregulated in these inflammatory conditions which leads to increased lymphocyte accumulation in the gut. This condition exacerbates the inflammation and tissue damage characteristic of these disorders. MECA-79 a monoclonal antibody targets MAdCAM-1 to aid therapeutic approaches in mitigating inflammation driven by lymphocyte infiltration. Understanding MAdCAM-1 and its function offers potential pathways to target and treat these chronic inflammatory diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Multiplex immunohistochemistry - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Multiplex immunohistochemistry - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) Rat lymph node tissue labelling MAdCAM1 with ab309487 at 1:100 dilution (B), CD3 epsilon with ab237721at 1:2000 dilution (C) and CD20 with Anti-CD20 antibody [SP32] ab64088 at 1:100 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-MAdCAM (green; Opal™520), anti-CD3 (gray; Opal™690) and anti-CD20 (magenta; Opal™570) on rat lymph node.

    Panel B: anti-MAdCAM staining high endothelial venules in rat lymph node.

    Panel C: ant-CD3 staining B lymphocytes in rat lymph node.

    Panel D: ant-CD20 staining B lymphocytes in rat lymph node.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab309487, Anti-CD3 epsilon antibody [CAL57] ab237721 and Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Multiplex immunohistochemistry - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) Mouse lymph node tissue labelling MAdCAM1 with ab309487 at 1:100 dilution (B), CD3 epsilon with ab237721at 1:2000 dilution (C) and CD20 with Anti-CD20 antibody [SP32] ab64088 at 1:100 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-MAdCAM (green; Opal™520), anti-CD3 (gray; Opal™690) and anti-CD20 (magenta; Opal™570) on mouse lymph node.

    Panel B: anti-MAdCAM staining high endothelial venules in mouse lymph node.

    Panel C: ant-CD3 staining B lymphocytes in mouse lymph node.

    Panel D: ant-CD20 staining B lymphocytes in mouse lymph node.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab309487, Anti-CD3 epsilon antibody [CAL57] ab237721 and Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MAdCAM1 with ab309487 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with a mouse MADCAM1 expression vector containing a myc tag, but no staining in 293T cells transfected with a human MAdCAM1 expression vector containing a myc tag.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelioma endothelialpolyoma middle T antigen transformed) cells labelling MAdCAM1 with ab309487 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining after the treatment with 20 ng/ml TNF alpha for 24 hours in bEnd.3 cells.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling MAdCAM1 with ab309487 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat cardiac muscle. The section was incubated with ab309487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MAdCAM1 with ab309487 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cardiac muscle. The section was incubated with ab309487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunohistochemical analysis of paraffin-embedded Rat peripheral lymph tissue labeling MAdCAM1 with ab309487 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the high endothelial venules of peripheral lymph nodes in rat jejunum. The section was incubated with ab309487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAdCAM1 antibody [EPR27223-58] (ab309487)

    Immunohistochemical analysis of paraffin-embedded Mouse peripheral lym tissue labeling MAdCAM1 with ab309487 at 1/100 (5.07 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the high endothelial venules of peripheral lymph nodes in mouse jejunum. The section was incubated with ab309487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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